Different molecular weight forms of opioid receptors revealed by polyclonal antibodies
Polyclonal antibodies were raised against a purified opioid receptor from bovine brain (Cho, et. al., 1986), and shown to inhibit 3H-diprenorphine binding to this receptor in a dose-dependent fashion. These antibodies were then used to characterize opioid-binding material present in rat brain and in...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1988-01, Vol.150 (1), p.237-244 |
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| creator | Roy, S. Zhu, Y.X. Lee, N.M. Loh, H.H. |
| description | Polyclonal antibodies were raised against a purified opioid receptor from bovine brain (Cho, et. al., 1986), and shown to inhibit
3H-diprenorphine binding to this receptor in a dose-dependent fashion. These antibodies were then used to characterize opioid-binding material present in rat brain and in NG108-15 neuroblastoma-glioma hybrid cells. Western blot analysis revealed that the antibodies reacted with a single species of 58,000 molecular weight in rat brain membranes; this closely corresponds in size to the bovine opioid receptor used to raise the antibodies. In contrast, the polyclonal antibodies reacted with a 45,000 molecular weight species in NG108-15 neuroblastoma-glioma hybrid cells; moreover, this band was specifically reduced in NG108-15 cells in which opioid receptors had been down-regulated by incubation with D-ala
2-D-leu
5-enkephalin for 24 hours. Thus at least two distinct opioid receptor molecules have been identified, which have antigenic similarities. |
| doi_str_mv | 10.1016/0006-291X(88)90511-6 |
| format | Article |
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3H-diprenorphine binding to this receptor in a dose-dependent fashion. These antibodies were then used to characterize opioid-binding material present in rat brain and in NG108-15 neuroblastoma-glioma hybrid cells. Western blot analysis revealed that the antibodies reacted with a single species of 58,000 molecular weight in rat brain membranes; this closely corresponds in size to the bovine opioid receptor used to raise the antibodies. In contrast, the polyclonal antibodies reacted with a 45,000 molecular weight species in NG108-15 neuroblastoma-glioma hybrid cells; moreover, this band was specifically reduced in NG108-15 cells in which opioid receptors had been down-regulated by incubation with D-ala
2-D-leu
5-enkephalin for 24 hours. Thus at least two distinct opioid receptor molecules have been identified, which have antigenic similarities.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/0006-291X(88)90511-6</identifier><identifier>PMID: 2827667</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Antibodies - immunology ; Antibody Specificity ; Biological and medical sciences ; Brain Chemistry ; Cattle ; Cell Membrane - analysis ; Cell receptors ; Cell structures and functions ; Diprenorphine - metabolism ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Glioma - analysis ; Hybrid Cells - analysis ; Immunization ; Immunosorbent Techniques ; Miscellaneous ; Molecular and cellular biology ; Neuroblastoma - analysis ; Rabbits - immunology ; Rats ; Receptors, Opioid - analysis ; Receptors, Opioid - immunology ; Receptors, Opioid - metabolism</subject><ispartof>Biochemical and biophysical research communications, 1988-01, Vol.150 (1), p.237-244</ispartof><rights>1988</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-5b23a6c8b319bbc69aa7dff4d01d647d98122aa7014d6a6fd468eb96bf2181043</citedby><cites>FETCH-LOGICAL-c418t-5b23a6c8b319bbc69aa7dff4d01d647d98122aa7014d6a6fd468eb96bf2181043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0006291X88905116$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19556260$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2827667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roy, S.</creatorcontrib><creatorcontrib>Zhu, Y.X.</creatorcontrib><creatorcontrib>Lee, N.M.</creatorcontrib><creatorcontrib>Loh, H.H.</creatorcontrib><title>Different molecular weight forms of opioid receptors revealed by polyclonal antibodies</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Polyclonal antibodies were raised against a purified opioid receptor from bovine brain (Cho, et. al., 1986), and shown to inhibit
3H-diprenorphine binding to this receptor in a dose-dependent fashion. These antibodies were then used to characterize opioid-binding material present in rat brain and in NG108-15 neuroblastoma-glioma hybrid cells. Western blot analysis revealed that the antibodies reacted with a single species of 58,000 molecular weight in rat brain membranes; this closely corresponds in size to the bovine opioid receptor used to raise the antibodies. In contrast, the polyclonal antibodies reacted with a 45,000 molecular weight species in NG108-15 neuroblastoma-glioma hybrid cells; moreover, this band was specifically reduced in NG108-15 cells in which opioid receptors had been down-regulated by incubation with D-ala
2-D-leu
5-enkephalin for 24 hours. Thus at least two distinct opioid receptor molecules have been identified, which have antigenic similarities.</description><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Brain Chemistry</subject><subject>Cattle</subject><subject>Cell Membrane - analysis</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Diprenorphine - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glioma - analysis</subject><subject>Hybrid Cells - analysis</subject><subject>Immunization</subject><subject>Immunosorbent Techniques</subject><subject>Miscellaneous</subject><subject>Molecular and cellular biology</subject><subject>Neuroblastoma - analysis</subject><subject>Rabbits - immunology</subject><subject>Rats</subject><subject>Receptors, Opioid - analysis</subject><subject>Receptors, Opioid - immunology</subject><subject>Receptors, Opioid - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo6-zqP1DIRdFDayrTnU4ugqzrByx4UfEW8lHRSLrTJj0r8-_NOMN601OK1FMvVQ8hj4C9AAbiJWNMdFzB12dSPldsAOjEHbIBpljHgfV3yeYWuU_Oa_3BGEAv1Bk545KPQowb8uVNDAELziudckK3S6bQXxi_fV9pyGWqNAeal5ijpwUdLmsutVU3aBJ6avd0yWnvUp5NomZeo80-Yn1A7gWTKj48vRfk89urT5fvu-uP7z5cvr7uXA9y7QbLt0Y4abegrHVCGTP6EHrPwIt-9EoC5-2PQe-FEcH3QqJVwgYOsp24vSBPj7lLyT93WFc9xeowJTNj3lU9Sqa4Uvy_IAxcAkjZwP4IupJrLRj0UuJkyl4D0wfv-iBVH6RqKfUf71q0scen_J2d0N8OnUS3_pNT31RnUihmdrH-zVbDILhgjXt15LBZu4lYdHURZ4c-Nv2r9jn-e5HfuEKf0Q</recordid><startdate>19880115</startdate><enddate>19880115</enddate><creator>Roy, S.</creator><creator>Zhu, Y.X.</creator><creator>Lee, N.M.</creator><creator>Loh, H.H.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19880115</creationdate><title>Different molecular weight forms of opioid receptors revealed by polyclonal antibodies</title><author>Roy, S. ; Zhu, Y.X. ; Lee, N.M. ; Loh, H.H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-5b23a6c8b319bbc69aa7dff4d01d647d98122aa7014d6a6fd468eb96bf2181043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Brain Chemistry</topic><topic>Cattle</topic><topic>Cell Membrane - analysis</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Diprenorphine - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glioma - analysis</topic><topic>Hybrid Cells - analysis</topic><topic>Immunization</topic><topic>Immunosorbent Techniques</topic><topic>Miscellaneous</topic><topic>Molecular and cellular biology</topic><topic>Neuroblastoma - analysis</topic><topic>Rabbits - immunology</topic><topic>Rats</topic><topic>Receptors, Opioid - analysis</topic><topic>Receptors, Opioid - immunology</topic><topic>Receptors, Opioid - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roy, S.</creatorcontrib><creatorcontrib>Zhu, Y.X.</creatorcontrib><creatorcontrib>Lee, N.M.</creatorcontrib><creatorcontrib>Loh, H.H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roy, S.</au><au>Zhu, Y.X.</au><au>Lee, N.M.</au><au>Loh, H.H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Different molecular weight forms of opioid receptors revealed by polyclonal antibodies</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1988-01-15</date><risdate>1988</risdate><volume>150</volume><issue>1</issue><spage>237</spage><epage>244</epage><pages>237-244</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Polyclonal antibodies were raised against a purified opioid receptor from bovine brain (Cho, et. al., 1986), and shown to inhibit
3H-diprenorphine binding to this receptor in a dose-dependent fashion. These antibodies were then used to characterize opioid-binding material present in rat brain and in NG108-15 neuroblastoma-glioma hybrid cells. Western blot analysis revealed that the antibodies reacted with a single species of 58,000 molecular weight in rat brain membranes; this closely corresponds in size to the bovine opioid receptor used to raise the antibodies. In contrast, the polyclonal antibodies reacted with a 45,000 molecular weight species in NG108-15 neuroblastoma-glioma hybrid cells; moreover, this band was specifically reduced in NG108-15 cells in which opioid receptors had been down-regulated by incubation with D-ala
2-D-leu
5-enkephalin for 24 hours. Thus at least two distinct opioid receptor molecules have been identified, which have antigenic similarities.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2827667</pmid><doi>10.1016/0006-291X(88)90511-6</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies - immunology Antibody Specificity Biological and medical sciences Brain Chemistry Cattle Cell Membrane - analysis Cell receptors Cell structures and functions Diprenorphine - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Glioma - analysis Hybrid Cells - analysis Immunization Immunosorbent Techniques Miscellaneous Molecular and cellular biology Neuroblastoma - analysis Rabbits - immunology Rats Receptors, Opioid - analysis Receptors, Opioid - immunology Receptors, Opioid - metabolism |
| title | Different molecular weight forms of opioid receptors revealed by polyclonal antibodies |
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