Isolation of a full-length complementary DNA coding for human E1 alpha subunit of the pyruvate dehydrogenase complex

A 1.5-kilobase cDNA clone for human pyruvate dehydrogenase E1 was isolated from a lambda gt11 expression library by screening with polyclonal antiserum to the E1 alpha subunit of the porcine pyruvate dehydrogenase complex, a polyclonal antibody against bovine pyruvate dehydrogenase complex and a syn...

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Veröffentlicht in:	The Journal of biological chemistry 1988-02, Vol.263 (4), p.1991-1995
Hauptverfasser:	De Meirleir, L, MacKay, N, Lam Hon Wah, A M, Robinson, B H
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acids - analysis Base Sequence Biological and medical sciences Biotechnology Carcinoma, Hepatocellular - enzymology Carcinoma, Hepatocellular - genetics DNA - isolation & purification Fundamental and applied biological sciences. Psychology Genes. Genome Genetic engineering Genetic technics Humans Liver Neoplasms - enzymology Liver Neoplasms - genetics Macromolecular Substances Methods. Procedures. Technologies Molecular and cellular biology Molecular cloning Molecular genetics Molecular Sequence Data Molecular Weight Pyruvate Dehydrogenase Complex - genetics Tumor Cells, Cultured - enzymology
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