Structure determination of a monoclonal Fab fragment specific for histidine-containing protein of the phosphoenolpyruvate: sugar phosphotransferase system of Escherichia coli

Jel 42 is a monoclonal antibody specific for histidine-containing protein, a small phosphocarrier protein required for sugar transport in Escherichia coli. Fab fragments prepared from this antibody by papain digestion consisted of three major isoelectric forms which were separated on a chromatofocus...

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Veröffentlicht in:	The Journal of biological chemistry 1988-02, Vol.263 (5), p.2571-2574
Hauptverfasser:	Prasad, L, Vandonselaar, M, Lee, J S, Delbaere, L T
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal - analysis Bacterial Proteins Biological and medical sciences Crystalline structure Crystallization Escherichia coli Escherichia coli - enzymology Fab Fundamental and applied biological sciences. Psychology Immunoglobulin Fab Fragments - analysis Isoelectric Focusing Models, Molecular Molecular biophysics monoclonal antibodies Phosphoenolpyruvate Sugar Phosphotransferase System - immunology phosphotransferase Structure in molecular biology sugar
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