A quantitative fluorescence-imaging technique for studying acetylcholine receptor turnover at neuromuscular junctions in living animals

We have developed a technique to measure changes in the amount of fluorescently labeled acetylcholine receptors in living muscles over long time periods. The measurements of fluorescence are made relative to a novel, photolytically stable fluorescence standard (Spectralon) which allows changes in fl...

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Veröffentlicht in:	Journal of neuroscience methods 1996-02, Vol.64 (2), p.199-208
Hauptverfasser:	Turney, Stephen G., Culican, Susan M., Lichtman, Jeff W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcholine receptor Animals Biological and medical sciences Cytophotometry - methods Fluorescence standard Fluorescent Dyes Fundamental and applied biological sciences. Psychology General aspects. Models. Methods Image Processing, Computer-Assisted Linear Models Mice Microfluorometry Microscopy, Fluorescence - instrumentation Neuromuscular junction Neuromuscular Junction - metabolism Quantitative fluorescence microscopy Receptors, Cholinergic - metabolism Reference Standards Signal Processing, Computer-Assisted - instrumentation SIT video camera Spectralon Vertebrates: nervous system and sense organs α-Bungarotoxin
Online-Zugang:	Volltext
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