Use of a Modified Tryptophanase Promoter to Direct High-Level Expression of Foreign Proteins in E. coli

We have modified the tryptophanase promoter (PtnaA) for use as a temperature-independent promoter for the production of recombinant proteins. Although any protein will have a temperature range in which its expression is optimal, we find the tryptophanase promoter functions at all physiologically rel...

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Veröffentlicht in:	Annals of the New York Academy of Sciences 1996-05, Vol.782 (1), p.297-310
Hauptverfasser:	SITNEY, KAREN C., MANN, MICHAEL B., STEARNS, GEORGE W., MENJARES, ANTHONY D., STEVENSON, JANICE L., SNAVELY, MARSHALL D., FIESCHKO, JOHN C., CURLESS, CRAIG, TSAI, LARRY B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Blotting, Western Cloning, Molecular - methods Cyclic AMP Receptor Protein - biosynthesis Cyclic AMP Receptor Protein - genetics Escherichia coli - genetics Fermentation Gene Expression Glucose - metabolism Molecular Sequence Data Promoter Regions, Genetic Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Restriction Mapping Sequence Deletion Temperature Tryptophanase - genetics
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