Hormonal regulation of estradiol biosynthesis, aromatase activity, and aromatase mRNA in rat ovarian follicles and corpora lutea
To determine the molecular basis for changes in aromatase (P450arom) activity in rat ovarian follicles and corpora lutea, seven clones for rat P450arom cDNA have been identified and isolated from a rat granulosa cell λgtll cDNA expression library using a 62 mer deoxyoligonucleotide probe (derived fr...
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| Veröffentlicht in: | Steroids 1987-10, Vol.50 (4), p.393-409 |
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| creator | Richards, JoAnne S. Hickey, Gerard J. Chen, Shiuan Shively, John E. Hall, Peter F. Gaddy-Kurten, Dana Kurten, Richard |
| description | To determine the molecular basis for changes in aromatase (P450arom) activity in rat ovarian follicles and corpora lutea, seven clones for rat P450arom cDNA have been identified and isolated from a rat granulosa cell λgtll cDNA expression library using a 62 mer deoxyoligonucleotide probe (derived from an amino acid sequence of purified human placental aromatase) and a human placental P450arom cDNA probe. One of the rat P450arom cDNA clones contained an insert 1.2 kb in size. Both the human 1.8 kb cDNA and the rat 1.2 kb cDNA probes hybridized to a single species of P450arom mRNA that was 2.6 kb in size. Northern blot analysis revealed that corpora lutea isolated on day 15 of pregnancy contained high amounts of P450arom mRNA, whereas granulosa cells of antral follicles of hormonally primed, hypophysectomized rats (i.e., those from which mRNA was isolated to construct the cDNA library) contained only low amounts of P450arom mRNA. The lower amounts of P450arom in granulosa cells of preovulatory follicles in the estradiol-follicle-stimulating hormone primed hypophysectomized rats were unexpected because follicles incubated in medium containing testosterone substrate produce more estradiol than do corpora lutea isolated on day 15 of pregnancy and incubated under similar conditions. Additional studies will determine the hormonal events responsible for the elevated amounts and constitutive maintenance of P450arom mRNA and aromatase activity in luteal cells
in vivo and
in
vitro. |
| doi_str_mv | 10.1016/0039-128X(87)90027-4 |
| format | Article |
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in vivo and
in
vitro.</description><identifier>ISSN: 0039-128X</identifier><identifier>EISSN: 1878-5867</identifier><identifier>DOI: 10.1016/0039-128X(87)90027-4</identifier><identifier>PMID: 3144064</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Aromatase - genetics ; Aromatase - metabolism ; Blotting, Northern ; Cells, Cultured ; Cloning, Molecular ; Corpus Luteum - enzymology ; Corpus Luteum - metabolism ; Estradiol - biosynthesis ; Female ; Follicle Stimulating Hormone - physiology ; Granulosa Cells - physiology ; Luteinizing Hormone - physiology ; Ovarian Follicle - enzymology ; Ovarian Follicle - metabolism ; Pregnancy ; Rats ; RNA, Messenger - analysis ; Theca Cells - physiology</subject><ispartof>Steroids, 1987-10, Vol.50 (4), p.393-409</ispartof><rights>1988</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-a37bcde25e37ccc0c0dd921f779569be092eaac1e90fc595766f86a0adb69ad53</citedby><cites>FETCH-LOGICAL-c338t-a37bcde25e37ccc0c0dd921f779569be092eaac1e90fc595766f86a0adb69ad53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0039128X87900274$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3144064$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Richards, JoAnne S.</creatorcontrib><creatorcontrib>Hickey, Gerard J.</creatorcontrib><creatorcontrib>Chen, Shiuan</creatorcontrib><creatorcontrib>Shively, John E.</creatorcontrib><creatorcontrib>Hall, Peter F.</creatorcontrib><creatorcontrib>Gaddy-Kurten, Dana</creatorcontrib><creatorcontrib>Kurten, Richard</creatorcontrib><title>Hormonal regulation of estradiol biosynthesis, aromatase activity, and aromatase mRNA in rat ovarian follicles and corpora lutea</title><title>Steroids</title><addtitle>Steroids</addtitle><description>To determine the molecular basis for changes in aromatase (P450arom) activity in rat ovarian follicles and corpora lutea, seven clones for rat P450arom cDNA have been identified and isolated from a rat granulosa cell λgtll cDNA expression library using a 62 mer deoxyoligonucleotide probe (derived from an amino acid sequence of purified human placental aromatase) and a human placental P450arom cDNA probe. One of the rat P450arom cDNA clones contained an insert 1.2 kb in size. Both the human 1.8 kb cDNA and the rat 1.2 kb cDNA probes hybridized to a single species of P450arom mRNA that was 2.6 kb in size. Northern blot analysis revealed that corpora lutea isolated on day 15 of pregnancy contained high amounts of P450arom mRNA, whereas granulosa cells of antral follicles of hormonally primed, hypophysectomized rats (i.e., those from which mRNA was isolated to construct the cDNA library) contained only low amounts of P450arom mRNA. The lower amounts of P450arom in granulosa cells of preovulatory follicles in the estradiol-follicle-stimulating hormone primed hypophysectomized rats were unexpected because follicles incubated in medium containing testosterone substrate produce more estradiol than do corpora lutea isolated on day 15 of pregnancy and incubated under similar conditions. Additional studies will determine the hormonal events responsible for the elevated amounts and constitutive maintenance of P450arom mRNA and aromatase activity in luteal cells
in vivo and
in
vitro.</description><subject>Animals</subject><subject>Aromatase - genetics</subject><subject>Aromatase - metabolism</subject><subject>Blotting, Northern</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>Corpus Luteum - enzymology</subject><subject>Corpus Luteum - metabolism</subject><subject>Estradiol - biosynthesis</subject><subject>Female</subject><subject>Follicle Stimulating Hormone - physiology</subject><subject>Granulosa Cells - physiology</subject><subject>Luteinizing Hormone - physiology</subject><subject>Ovarian Follicle - enzymology</subject><subject>Ovarian Follicle - metabolism</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>RNA, Messenger - analysis</subject><subject>Theca Cells - physiology</subject><issn>0039-128X</issn><issn>1878-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kN1rFDEUxUOx1G31P6iQJ1HoaDIf-XgRSrEfUBSkBd_CneSORjKTNcks7Jt_urPdpfjk04Vzz7mX8yPknLMPnHHxkbFGV7xW398p-V4zVsuqPSIrrqSqOiXkC7J6trwkpzn_YoyJRtcn5KThbctEuyJ_bmMa4wSBJvwxByg-TjQOFHNJ4HwMtPcxb6fyE7PPFxRSHKFARgq2-I0v20Wb3D_6-O3LJfUTTVBo3EDyMNEhhuBtwPzktTGtYwIa5oLwihwPEDK-Pswz8nj9-eHqtrr_enN3dXlf2aZRpYJG9tZh3WEjrbXMMud0zQcpdSd0j0zXCGA5ajbYTndSiEEJYOB6ocF1zRl5u7-7TvH3vNQzo88WQ4AJ45yNVEwI3orF2O6NNsWcEw5mnfwIaWs4MzvwZkfV7KgaJc0TeNMusTeH-3M_onsOHUgv-0_7PS4lNx6TydbjZNH5hLYYF_3_H_wFBZaV2g</recordid><startdate>19871001</startdate><enddate>19871001</enddate><creator>Richards, JoAnne S.</creator><creator>Hickey, Gerard J.</creator><creator>Chen, Shiuan</creator><creator>Shively, John E.</creator><creator>Hall, Peter F.</creator><creator>Gaddy-Kurten, Dana</creator><creator>Kurten, Richard</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19871001</creationdate><title>Hormonal regulation of estradiol biosynthesis, aromatase activity, and aromatase mRNA in rat ovarian follicles and corpora lutea</title><author>Richards, JoAnne S. ; Hickey, Gerard J. ; Chen, Shiuan ; Shively, John E. ; Hall, Peter F. ; Gaddy-Kurten, Dana ; Kurten, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-a37bcde25e37ccc0c0dd921f779569be092eaac1e90fc595766f86a0adb69ad53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Animals</topic><topic>Aromatase - genetics</topic><topic>Aromatase - metabolism</topic><topic>Blotting, Northern</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>Corpus Luteum - enzymology</topic><topic>Corpus Luteum - metabolism</topic><topic>Estradiol - biosynthesis</topic><topic>Female</topic><topic>Follicle Stimulating Hormone - physiology</topic><topic>Granulosa Cells - physiology</topic><topic>Luteinizing Hormone - physiology</topic><topic>Ovarian Follicle - enzymology</topic><topic>Ovarian Follicle - metabolism</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>RNA, Messenger - analysis</topic><topic>Theca Cells - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Richards, JoAnne S.</creatorcontrib><creatorcontrib>Hickey, Gerard J.</creatorcontrib><creatorcontrib>Chen, Shiuan</creatorcontrib><creatorcontrib>Shively, John E.</creatorcontrib><creatorcontrib>Hall, Peter F.</creatorcontrib><creatorcontrib>Gaddy-Kurten, Dana</creatorcontrib><creatorcontrib>Kurten, Richard</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Richards, JoAnne S.</au><au>Hickey, Gerard J.</au><au>Chen, Shiuan</au><au>Shively, John E.</au><au>Hall, Peter F.</au><au>Gaddy-Kurten, Dana</au><au>Kurten, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hormonal regulation of estradiol biosynthesis, aromatase activity, and aromatase mRNA in rat ovarian follicles and corpora lutea</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>1987-10-01</date><risdate>1987</risdate><volume>50</volume><issue>4</issue><spage>393</spage><epage>409</epage><pages>393-409</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><abstract>To determine the molecular basis for changes in aromatase (P450arom) activity in rat ovarian follicles and corpora lutea, seven clones for rat P450arom cDNA have been identified and isolated from a rat granulosa cell λgtll cDNA expression library using a 62 mer deoxyoligonucleotide probe (derived from an amino acid sequence of purified human placental aromatase) and a human placental P450arom cDNA probe. One of the rat P450arom cDNA clones contained an insert 1.2 kb in size. Both the human 1.8 kb cDNA and the rat 1.2 kb cDNA probes hybridized to a single species of P450arom mRNA that was 2.6 kb in size. Northern blot analysis revealed that corpora lutea isolated on day 15 of pregnancy contained high amounts of P450arom mRNA, whereas granulosa cells of antral follicles of hormonally primed, hypophysectomized rats (i.e., those from which mRNA was isolated to construct the cDNA library) contained only low amounts of P450arom mRNA. The lower amounts of P450arom in granulosa cells of preovulatory follicles in the estradiol-follicle-stimulating hormone primed hypophysectomized rats were unexpected because follicles incubated in medium containing testosterone substrate produce more estradiol than do corpora lutea isolated on day 15 of pregnancy and incubated under similar conditions. Additional studies will determine the hormonal events responsible for the elevated amounts and constitutive maintenance of P450arom mRNA and aromatase activity in luteal cells
in vivo and
in
vitro.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>3144064</pmid><doi>10.1016/0039-128X(87)90027-4</doi><tpages>17</tpages></addata></record> |
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| identifier | ISSN: 0039-128X |
| ispartof | Steroids, 1987-10, Vol.50 (4), p.393-409 |
| issn | 0039-128X 1878-5867 |
| language | eng |
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| subjects | Animals Aromatase - genetics Aromatase - metabolism Blotting, Northern Cells, Cultured Cloning, Molecular Corpus Luteum - enzymology Corpus Luteum - metabolism Estradiol - biosynthesis Female Follicle Stimulating Hormone - physiology Granulosa Cells - physiology Luteinizing Hormone - physiology Ovarian Follicle - enzymology Ovarian Follicle - metabolism Pregnancy Rats RNA, Messenger - analysis Theca Cells - physiology |
| title | Hormonal regulation of estradiol biosynthesis, aromatase activity, and aromatase mRNA in rat ovarian follicles and corpora lutea |
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