Mass allele detection (MAD) of rare 5-HT1A structural variants with allele-specific amplification and electrochemiluminescent detection

A strategy is described that exploits allele‐specific amplification (ASA‐PCR) and electrochemiluminescence (ECL) detection technology to rapidly and cheaply screen large numbers of DNAs arranged in pooled matrices in order to identify individual nucleotide sequence variants. To demonstrate this stra...

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Veröffentlicht in:	Human mutation 1996, Vol.7 (2), p.135-143
Hauptverfasser:	Bergen, Andrew, Wang, Chang-Yu, Nakhai, Bita, Goldman, David
Format:	Artikel
Sprache:	eng
Schlagworte:	Allele detection Alleles ASA-PCR Base Sequence Biotin Diagnostic screening method DNA Primers - chemistry ECL Electrophoresis, Polyacrylamide Gel Ethnic Groups - genetics Genetic Testing - methods Genetic Variation Heterozygote Humans Luminescent Measurements Molecular Sequence Data Mutation Polymerase Chain Reaction Polymorphism, Genetic - genetics Polymorphism, Single-Stranded Conformational Receptors, Serotonin - chemistry Receptors, Serotonin - genetics Receptors, Serotonin, 5-HT1 Sensitivity and Specificity
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