Routine clinical determination of carotene, vitamin E, vitamin A, 25-hydroxy vitamin D3 and trans-vitamin K1 in human serum by straight phase HPLC

A universal extraction procedure is described for fat‐soluble vitamins in human serum. Methods are presented for routine quantitative analysis by isocratic straight phase HPLC with UV‐detection of (α + β)‐carotene, vitamin E. (α‐tocopherol) and vitamin A (all‐trans‐retinol) in one single run, and of...

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Veröffentlicht in:	Biomedical chromatography 1987, Vol.2 (2), p.79-88
Hauptverfasser:	Van Haard, Paul M. M., Engel, Roel, Postma, Tjeerd
Format:	Artikel
Sprache:	eng
Schlagworte:	Calcifediol - blood Carotenoids - blood Chromatography, High Pressure Liquid - methods Humans Vitamin A - blood Vitamin E - blood Vitamin K 1 - blood Vitamins - blood
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container_title	Biomedical chromatography
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creator	Van Haard, Paul M. M. Engel, Roel Postma, Tjeerd
description	A universal extraction procedure is described for fat‐soluble vitamins in human serum. Methods are presented for routine quantitative analysis by isocratic straight phase HPLC with UV‐detection of (α + β)‐carotene, vitamin E. (α‐tocopherol) and vitamin A (all‐trans‐retinol) in one single run, and of vitamin K1 (trans‐phylloquinone) and 25‐hydroxy vitamin D3 after sample clean‐up using disposable reversed‐phase cartridges. The limits of detection, precisions and selectivities of the developed assays are shown to be satisfactory after more than three years' experience. The routine clinical determination of fat‐soluble vitamins can be performed in less than 5 mL of serum. Analyses of external quality control and randomly taken outpatient samples are shown to be of great value in assessing laboratory performance.
doi_str_mv	10.1002/bmc.1130020209
format	Article
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M.</creatorcontrib><creatorcontrib>Engel, Roel</creatorcontrib><creatorcontrib>Postma, Tjeerd</creatorcontrib><title>Routine clinical determination of carotene, vitamin E, vitamin A, 25-hydroxy vitamin D3 and trans-vitamin K1 in human serum by straight phase HPLC</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>A universal extraction procedure is described for fat‐soluble vitamins in human serum. Methods are presented for routine quantitative analysis by isocratic straight phase HPLC with UV‐detection of (α + β)‐carotene, vitamin E. (α‐tocopherol) and vitamin A (all‐trans‐retinol) in one single run, and of vitamin K1 (trans‐phylloquinone) and 25‐hydroxy vitamin D3 after sample clean‐up using disposable reversed‐phase cartridges. The limits of detection, precisions and selectivities of the developed assays are shown to be satisfactory after more than three years' experience. The routine clinical determination of fat‐soluble vitamins can be performed in less than 5 mL of serum. Analyses of external quality control and randomly taken outpatient samples are shown to be of great value in assessing laboratory performance.</description><subject>Calcifediol - blood</subject><subject>Carotenoids - blood</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Humans</subject><subject>Vitamin A - blood</subject><subject>Vitamin E - blood</subject><subject>Vitamin K 1 - blood</subject><subject>Vitamins - blood</subject><issn>0269-3879</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUUtvEzEQthCohMKVG5JPnLrBXu_a8bENbYMID1WRKnGxZr02Mex6U9sL3b_BL8YoIRUnNJJnNN9DGn8IvaRkTgkp3zS9nlPK8phLPkIzSqQsyILQx2hGSi4LthDyKXoW4zdCiOSlOEEnrCZ8weQM_boZxuS8wbpz3mnocGuSCb3zkNzg8WCxhjAk480Z_uESZARfPoznZ7isi-3UhuF-Om7fMgy-xSmAj8Xf5XuK87sde_A4mjD2uJlwzBz3dZvwbgvR4NXn9fI5emKhi-bFoZ-izdXlZrkq1p-u3y3P14VmdT6x0qJiUHHKODRgyhqM1ZRXmkLVcsEt5VxaY6WwJdQcQNeSNGAryi2Blp2i13vbXRjuRhOT6l3UpuvAm2GMSiwIL-tSZOJ8T9RhiDEYq3bB9RAmRYn6k4HKGaiHDLLg1cF5bHrTHumHT8-43OM_XWem_7ipiw_Lf7yLvdbFZO6PWgjfFRdM1Or247XaXJDbFb_5oq7Yb34qosI</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Van Haard, Paul M. M.</creator><creator>Engel, Roel</creator><creator>Postma, Tjeerd</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Routine clinical determination of carotene, vitamin E, vitamin A, 25-hydroxy vitamin D3 and trans-vitamin K1 in human serum by straight phase HPLC</title><author>Van Haard, Paul M. M. ; Engel, Roel ; Postma, Tjeerd</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3599-4c743a46136abae25aefc164c1a4d676f1669fef97f2a56aac590baf416f0ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Calcifediol - blood</topic><topic>Carotenoids - blood</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Humans</topic><topic>Vitamin A - blood</topic><topic>Vitamin E - blood</topic><topic>Vitamin K 1 - blood</topic><topic>Vitamins - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Haard, Paul M. M.</creatorcontrib><creatorcontrib>Engel, Roel</creatorcontrib><creatorcontrib>Postma, Tjeerd</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Haard, Paul M. M.</au><au>Engel, Roel</au><au>Postma, Tjeerd</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Routine clinical determination of carotene, vitamin E, vitamin A, 25-hydroxy vitamin D3 and trans-vitamin K1 in human serum by straight phase HPLC</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>1987</date><risdate>1987</risdate><volume>2</volume><issue>2</issue><spage>79</spage><epage>88</epage><pages>79-88</pages><issn>0269-3879</issn><eissn>1099-0801</eissn><abstract>A universal extraction procedure is described for fat‐soluble vitamins in human serum. Methods are presented for routine quantitative analysis by isocratic straight phase HPLC with UV‐detection of (α + β)‐carotene, vitamin E. (α‐tocopherol) and vitamin A (all‐trans‐retinol) in one single run, and of vitamin K1 (trans‐phylloquinone) and 25‐hydroxy vitamin D3 after sample clean‐up using disposable reversed‐phase cartridges. The limits of detection, precisions and selectivities of the developed assays are shown to be satisfactory after more than three years' experience. The routine clinical determination of fat‐soluble vitamins can be performed in less than 5 mL of serum. 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source	MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects	Calcifediol - blood Carotenoids - blood Chromatography, High Pressure Liquid - methods Humans Vitamin A - blood Vitamin E - blood Vitamin K 1 - blood Vitamins - blood
title	Routine clinical determination of carotene, vitamin E, vitamin A, 25-hydroxy vitamin D3 and trans-vitamin K1 in human serum by straight phase HPLC
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