Quantitative analysis of hydroxyurea and urea by proton nuclear magnetic resonance (NMR) spectroscopy
Quantitative 1H-nuclear magnetic resonance (NMR) procedures are described for measuring hydroxyurea and urea. Dimethylsulfoxide-d6 is used as the solvent for both assays; the internal standards employed are urea and p-dichlorobenzene for hydroxyurea and urea, respectively. The analysis for hydroxyur...
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| Veröffentlicht in: | Pharmaceutical research 1987-10, Vol.4 (5), p.412-415 |
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| creator | MAIN, K. B MEDWICK, T BAILEY, L. C SHINKAI, J. H |
| description | Quantitative 1H-nuclear magnetic resonance (NMR) procedures are described for measuring hydroxyurea and urea. Dimethylsulfoxide-d6 is used as the solvent for both assays; the internal standards employed are urea and p-dichlorobenzene for hydroxyurea and urea, respectively. The analysis for hydroxyurea is based on the comparison of the area of the -NH2 peak of hydroxyurea with the area of the urea -NH2 peak area. The analysis of urea is based on the comparison of the -NH2 peak of urea with the area of the p-dichlorobenzene singlet. The 1H-NMR assays yield results that are precise and agree well with results of the more cumbersome and less specific USP procedures. |
| doi_str_mv | 10.1023/A:1016490430135 |
| format | Article |
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The 1H-NMR assays yield results that are precise and agree well with results of the more cumbersome and less specific USP procedures.</description><identifier>ISSN: 0724-8741</identifier><identifier>EISSN: 1573-904X</identifier><identifier>DOI: 10.1023/A:1016490430135</identifier><identifier>PMID: 3508551</identifier><identifier>CODEN: PHREEB</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Biological and medical sciences ; Hydroxyurea - analysis ; Magnetic Resonance Spectroscopy ; Medical sciences ; Pharmacology. 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C</creatorcontrib><creatorcontrib>SHINKAI, J. H</creatorcontrib><title>Quantitative analysis of hydroxyurea and urea by proton nuclear magnetic resonance (NMR) spectroscopy</title><title>Pharmaceutical research</title><addtitle>Pharm Res</addtitle><description>Quantitative 1H-nuclear magnetic resonance (NMR) procedures are described for measuring hydroxyurea and urea. Dimethylsulfoxide-d6 is used as the solvent for both assays; the internal standards employed are urea and p-dichlorobenzene for hydroxyurea and urea, respectively. The analysis for hydroxyurea is based on the comparison of the area of the -NH2 peak of hydroxyurea with the area of the urea -NH2 peak area. The analysis of urea is based on the comparison of the -NH2 peak of urea with the area of the p-dichlorobenzene singlet. The 1H-NMR assays yield results that are precise and agree well with results of the more cumbersome and less specific USP procedures.</description><subject>Biological and medical sciences</subject><subject>Hydroxyurea - analysis</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Medical sciences</subject><subject>Pharmacology. 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Drug treatments</topic><topic>Urea - analysis</topic><topic>Urinary system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MAIN, K. B</creatorcontrib><creatorcontrib>MEDWICK, T</creatorcontrib><creatorcontrib>BAILEY, L. C</creatorcontrib><creatorcontrib>SHINKAI, J. H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmaceutical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MAIN, K. B</au><au>MEDWICK, T</au><au>BAILEY, L. C</au><au>SHINKAI, J. H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative analysis of hydroxyurea and urea by proton nuclear magnetic resonance (NMR) spectroscopy</atitle><jtitle>Pharmaceutical research</jtitle><addtitle>Pharm Res</addtitle><date>1987-10-01</date><risdate>1987</risdate><volume>4</volume><issue>5</issue><spage>412</spage><epage>415</epage><pages>412-415</pages><issn>0724-8741</issn><eissn>1573-904X</eissn><coden>PHREEB</coden><abstract>Quantitative 1H-nuclear magnetic resonance (NMR) procedures are described for measuring hydroxyurea and urea. Dimethylsulfoxide-d6 is used as the solvent for both assays; the internal standards employed are urea and p-dichlorobenzene for hydroxyurea and urea, respectively. The analysis for hydroxyurea is based on the comparison of the area of the -NH2 peak of hydroxyurea with the area of the urea -NH2 peak area. The analysis of urea is based on the comparison of the -NH2 peak of urea with the area of the p-dichlorobenzene singlet. The 1H-NMR assays yield results that are precise and agree well with results of the more cumbersome and less specific USP procedures.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>3508551</pmid><doi>10.1023/A:1016490430135</doi><tpages>4</tpages></addata></record> |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_78060883 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Biological and medical sciences Hydroxyurea - analysis Magnetic Resonance Spectroscopy Medical sciences Pharmacology. Drug treatments Urea - analysis Urinary system |
| title | Quantitative analysis of hydroxyurea and urea by proton nuclear magnetic resonance (NMR) spectroscopy |
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