The cloning, expression and crystallisation of a thermostable arginase

The gene for the thermostable arginase from the thermophilic bacterium ‘ Bacillus caldovelox’ has been cloned and sequenced. Expression of recombinant arginase at high levels has been achieved in E. coli using an inducible T7 RNA polymerase-based system. A facile purification procedure incorporating...

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Veröffentlicht in:	FEBS letters 1996-05, Vol.386 (2), p.215-218
Hauptverfasser:	Bewley, Maria C., Lott, J.Shaun, Baker, Edward N., Patchett, Mark L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Arginase Arginase - chemistry Arginase - genetics Arginase - metabolism Bacillus - enzymology Bacillus caldovelox Base Sequence Cloning, Molecular Crystallography, X-Ray DNA, Bacterial Escherichia coli Gene cloning Gene Expression Hot Temperature Molecular Sequence Data Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Sequence Homology, Amino Acid Thermophilic Thermostable
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