Insulin-Like Growth Factor (IGF) System Components in Human Prostatic Cancer Cell-Lines: LNCaP, DU145, and PC-3 Cells
Background: Evidence has been accumulating that in many tumors, insulin‐like growth factors (ICFs) promote cancer cell growth in an autocrine/paracrine manner via the IGF‐1 receptor. In an effort to understand the role of ICFs in prostate cancer cell growth, we characterized the IGF system component...
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| Veröffentlicht in: | International journal of urology 1996-01, Vol.3 (1), p.39-46 |
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| creator | Kimura, Go Kasuya, Junko Giannini, Stefano Honda, Yoko Mohan, Subburaman Kawachi, Mark H. Akimoto, Masato Fujita-Yamaguchi, Yoko |
| description | Background: Evidence has been accumulating that in many tumors, insulin‐like growth factors (ICFs) promote cancer cell growth in an autocrine/paracrine manner via the IGF‐1 receptor. In an effort to understand the role of ICFs in prostate cancer cell growth, we characterized the IGF system components produced by human prostatic cancer cell‐lines, LNCaP, DU145, and PC‐3, grown in serum‐free medium.
Methods: IGFs, their receptors, and IGF binding proteins (IGFBPs) produced by the three human prostate cell lines were characterized by reverse transcriptase‐polymerase chain reaction (RT‐PCR), radioimmunoassay (RIA), Western ligand blot, Western immunoblot, and Northern blot analyses.
Results: mRNA for IGF‐II and receptors for IGF‐I and IGF‐II were detected in all three cell‐lines by RT‐PCR. In contrast to the published study, only LNCaP cells expressed a trace amount of IGF‐I mRNA. RIA on conditioned media collected from these cells revealed that all three cell‐lines produced measurable IGF‐II but not IGF‐I. Western ligand blot, Western immunoblot, and Northern blot analyses revealed that LNCaP, DU145, and PC‐3 cells expressed IGFBP‐2, IGFBP‐2/‐3/‐4/‐6, and IGFBP‐2/‐3/‐4/‐5/‐6, respectively. IGF‐II stimulated [3H]thymidine incorporation into DNA in DU145 and PC‐3 cells significantly although the effect was small. DNA synthesis in PC‐3 cells but not in LNCaP and DU145 cells was significantly inhibited by the IGF‐I receptor‐specific monoclonal antibody.
Conclusion: These results suggest potentially important roles of IGFs and IGFBPs in prostate cancer cell growth, and that in particular, IGF‐II may play a critical role in prostate cancer cell growth. |
| doi_str_mv | 10.1111/j.1442-2042.1996.tb00628.x |
| format | Article |
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Methods: IGFs, their receptors, and IGF binding proteins (IGFBPs) produced by the three human prostate cell lines were characterized by reverse transcriptase‐polymerase chain reaction (RT‐PCR), radioimmunoassay (RIA), Western ligand blot, Western immunoblot, and Northern blot analyses.
Results: mRNA for IGF‐II and receptors for IGF‐I and IGF‐II were detected in all three cell‐lines by RT‐PCR. In contrast to the published study, only LNCaP cells expressed a trace amount of IGF‐I mRNA. RIA on conditioned media collected from these cells revealed that all three cell‐lines produced measurable IGF‐II but not IGF‐I. Western ligand blot, Western immunoblot, and Northern blot analyses revealed that LNCaP, DU145, and PC‐3 cells expressed IGFBP‐2, IGFBP‐2/‐3/‐4/‐6, and IGFBP‐2/‐3/‐4/‐5/‐6, respectively. IGF‐II stimulated [3H]thymidine incorporation into DNA in DU145 and PC‐3 cells significantly although the effect was small. DNA synthesis in PC‐3 cells but not in LNCaP and DU145 cells was significantly inhibited by the IGF‐I receptor‐specific monoclonal antibody.
Conclusion: These results suggest potentially important roles of IGFs and IGFBPs in prostate cancer cell growth, and that in particular, IGF‐II may play a critical role in prostate cancer cell growth.</description><identifier>ISSN: 0919-8172</identifier><identifier>EISSN: 1442-2042</identifier><identifier>DOI: 10.1111/j.1442-2042.1996.tb00628.x</identifier><identifier>PMID: 8646598</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Antibodies, Monoclonal ; Base Sequence ; Blotting, Northern ; Cell Division - drug effects ; Cell Division - immunology ; cell growth ; Culture Media, Conditioned - chemistry ; Humans ; IGF-I receptor monoclonal antibody ; IGFBPs ; IGFs ; Insulin-Like Growth Factor Binding Protein 3 - analysis ; Insulin-Like Growth Factor Binding Protein 3 - genetics ; Insulin-Like Growth Factor Binding Protein 4 - genetics ; Insulin-Like Growth Factor Binding Protein 4 - metabolism ; Insulin-Like Growth Factor Binding Protein 5 - genetics ; Insulin-Like Growth Factor Binding Protein 5 - metabolism ; Insulin-Like Growth Factor Binding Protein 6 - genetics ; Insulin-Like Growth Factor Binding Protein 6 - metabolism ; Insulin-Like Growth Factor Binding Proteins - analysis ; Insulin-Like Growth Factor Binding Proteins - genetics ; Insulin-Like Growth Factor Binding Proteins - secretion ; Insulin-Like Growth Factor I - analysis ; Insulin-Like Growth Factor I - genetics ; Insulin-Like Growth Factor II - analysis ; Insulin-Like Growth Factor II - genetics ; Insulin-Like Growth Factor II - pharmacology ; Male ; Molecular Sequence Data ; prostate cancer cells ; Prostatic Neoplasms ; Receptor, IGF Type 1 - analysis ; Receptor, IGF Type 1 - genetics ; Receptor, IGF Type 1 - metabolism ; Receptor, IGF Type 2 - analysis ; Receptor, IGF Type 2 - genetics ; Receptor, IGF Type 2 - metabolism ; RNA, Messenger - analysis ; Tumor Cells, Cultured - chemistry ; Tumor Cells, Cultured - cytology ; Tumor Cells, Cultured - physiology</subject><ispartof>International journal of urology, 1996-01, Vol.3 (1), p.39-46</ispartof><rights>1996 Japanese Urological Association</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4698-b60e21af9f90ba08ca0158d0dc6a9744931077a4eaa0ba9077effad2d83b10fa3</citedby><cites>FETCH-LOGICAL-c4698-b60e21af9f90ba08ca0158d0dc6a9744931077a4eaa0ba9077effad2d83b10fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1442-2042.1996.tb00628.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1442-2042.1996.tb00628.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8646598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kimura, Go</creatorcontrib><creatorcontrib>Kasuya, Junko</creatorcontrib><creatorcontrib>Giannini, Stefano</creatorcontrib><creatorcontrib>Honda, Yoko</creatorcontrib><creatorcontrib>Mohan, Subburaman</creatorcontrib><creatorcontrib>Kawachi, Mark H.</creatorcontrib><creatorcontrib>Akimoto, Masato</creatorcontrib><creatorcontrib>Fujita-Yamaguchi, Yoko</creatorcontrib><title>Insulin-Like Growth Factor (IGF) System Components in Human Prostatic Cancer Cell-Lines: LNCaP, DU145, and PC-3 Cells</title><title>International journal of urology</title><addtitle>Int J Urol</addtitle><description>Background: Evidence has been accumulating that in many tumors, insulin‐like growth factors (ICFs) promote cancer cell growth in an autocrine/paracrine manner via the IGF‐1 receptor. In an effort to understand the role of ICFs in prostate cancer cell growth, we characterized the IGF system components produced by human prostatic cancer cell‐lines, LNCaP, DU145, and PC‐3, grown in serum‐free medium.
Methods: IGFs, their receptors, and IGF binding proteins (IGFBPs) produced by the three human prostate cell lines were characterized by reverse transcriptase‐polymerase chain reaction (RT‐PCR), radioimmunoassay (RIA), Western ligand blot, Western immunoblot, and Northern blot analyses.
Results: mRNA for IGF‐II and receptors for IGF‐I and IGF‐II were detected in all three cell‐lines by RT‐PCR. In contrast to the published study, only LNCaP cells expressed a trace amount of IGF‐I mRNA. RIA on conditioned media collected from these cells revealed that all three cell‐lines produced measurable IGF‐II but not IGF‐I. Western ligand blot, Western immunoblot, and Northern blot analyses revealed that LNCaP, DU145, and PC‐3 cells expressed IGFBP‐2, IGFBP‐2/‐3/‐4/‐6, and IGFBP‐2/‐3/‐4/‐5/‐6, respectively. IGF‐II stimulated [3H]thymidine incorporation into DNA in DU145 and PC‐3 cells significantly although the effect was small. DNA synthesis in PC‐3 cells but not in LNCaP and DU145 cells was significantly inhibited by the IGF‐I receptor‐specific monoclonal antibody.
Conclusion: These results suggest potentially important roles of IGFs and IGFBPs in prostate cancer cell growth, and that in particular, IGF‐II may play a critical role in prostate cancer cell growth.</description><subject>Antibodies, Monoclonal</subject><subject>Base Sequence</subject><subject>Blotting, Northern</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - immunology</subject><subject>cell growth</subject><subject>Culture Media, Conditioned - chemistry</subject><subject>Humans</subject><subject>IGF-I receptor monoclonal antibody</subject><subject>IGFBPs</subject><subject>IGFs</subject><subject>Insulin-Like Growth Factor Binding Protein 3 - analysis</subject><subject>Insulin-Like Growth Factor Binding Protein 3 - genetics</subject><subject>Insulin-Like Growth Factor Binding Protein 4 - genetics</subject><subject>Insulin-Like Growth Factor Binding Protein 4 - metabolism</subject><subject>Insulin-Like Growth Factor Binding Protein 5 - genetics</subject><subject>Insulin-Like Growth Factor Binding Protein 5 - metabolism</subject><subject>Insulin-Like Growth Factor Binding Protein 6 - genetics</subject><subject>Insulin-Like Growth Factor Binding Protein 6 - metabolism</subject><subject>Insulin-Like Growth Factor Binding Proteins - analysis</subject><subject>Insulin-Like Growth Factor Binding Proteins - genetics</subject><subject>Insulin-Like Growth Factor Binding Proteins - secretion</subject><subject>Insulin-Like Growth Factor I - analysis</subject><subject>Insulin-Like Growth Factor I - genetics</subject><subject>Insulin-Like Growth Factor II - analysis</subject><subject>Insulin-Like Growth Factor II - genetics</subject><subject>Insulin-Like Growth Factor II - pharmacology</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>prostate cancer cells</subject><subject>Prostatic Neoplasms</subject><subject>Receptor, IGF Type 1 - analysis</subject><subject>Receptor, IGF Type 1 - genetics</subject><subject>Receptor, IGF Type 1 - metabolism</subject><subject>Receptor, IGF Type 2 - analysis</subject><subject>Receptor, IGF Type 2 - genetics</subject><subject>Receptor, IGF Type 2 - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>Tumor Cells, Cultured - chemistry</subject><subject>Tumor Cells, Cultured - cytology</subject><subject>Tumor Cells, Cultured - physiology</subject><issn>0919-8172</issn><issn>1442-2042</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkdFq2zAUhsVYabN2j1AQvRgd1K5ky7LUi8JwlzRbyAJrKPRGHNsyc2rLmWTT5O2nNCH3040O_Od8kj4hdEVJSP26XYWUsSiICItCKiUP-5wQHolw8wGNjtFHNCKSykDQNDpDn5xbEULjiIpTdCo444kUIzRMjRua2gSz-lXjie3e-j94DEXfWXw9nYy_4t9b1-sWZ1277ow2vcO1wY9DCwYvbOd66OsCZ2AKbXGmm8aTjHZ3eDbPYHGDH5aUJTcYTIkXWRC_t7gLdFJB4_Tnw36OluPvT9ljMPs1mWbfZkHBuBRBzomOKFSykiQHIgogNBElKQsOMmVMxpSkKTAN4HPpa11VUEaliHNKKojP0Zc9d227v4N2vWprV_gbgNHd4FQqSMLThPvGu31j4Z_krK7U2tYt2K2iRO2cq5XaiVU7sWrnXB2cq40fvjycMuStLo-jB8k-v9_nb3Wjt_9BVtMfS194QLAH1P4rNkcA2FfF0zhN1PN8ovjLw8854UKx-B-eE54Z</recordid><startdate>199601</startdate><enddate>199601</enddate><creator>Kimura, Go</creator><creator>Kasuya, Junko</creator><creator>Giannini, Stefano</creator><creator>Honda, Yoko</creator><creator>Mohan, Subburaman</creator><creator>Kawachi, Mark H.</creator><creator>Akimoto, Masato</creator><creator>Fujita-Yamaguchi, Yoko</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199601</creationdate><title>Insulin-Like Growth Factor (IGF) System Components in Human Prostatic Cancer Cell-Lines: LNCaP, DU145, and PC-3 Cells</title><author>Kimura, Go ; Kasuya, Junko ; Giannini, Stefano ; Honda, Yoko ; Mohan, Subburaman ; Kawachi, Mark H. ; Akimoto, Masato ; Fujita-Yamaguchi, Yoko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4698-b60e21af9f90ba08ca0158d0dc6a9744931077a4eaa0ba9077effad2d83b10fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antibodies, Monoclonal</topic><topic>Base Sequence</topic><topic>Blotting, Northern</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - immunology</topic><topic>cell growth</topic><topic>Culture Media, Conditioned - chemistry</topic><topic>Humans</topic><topic>IGF-I receptor monoclonal antibody</topic><topic>IGFBPs</topic><topic>IGFs</topic><topic>Insulin-Like Growth Factor Binding Protein 3 - analysis</topic><topic>Insulin-Like Growth Factor Binding Protein 3 - genetics</topic><topic>Insulin-Like Growth Factor Binding Protein 4 - genetics</topic><topic>Insulin-Like Growth Factor Binding Protein 4 - metabolism</topic><topic>Insulin-Like Growth Factor Binding Protein 5 - genetics</topic><topic>Insulin-Like Growth Factor Binding Protein 5 - metabolism</topic><topic>Insulin-Like Growth Factor Binding Protein 6 - genetics</topic><topic>Insulin-Like Growth Factor Binding Protein 6 - metabolism</topic><topic>Insulin-Like Growth Factor Binding Proteins - analysis</topic><topic>Insulin-Like Growth Factor Binding Proteins - genetics</topic><topic>Insulin-Like Growth Factor Binding Proteins - secretion</topic><topic>Insulin-Like Growth Factor I - analysis</topic><topic>Insulin-Like Growth Factor I - genetics</topic><topic>Insulin-Like Growth Factor II - analysis</topic><topic>Insulin-Like Growth Factor II - genetics</topic><topic>Insulin-Like Growth Factor II - pharmacology</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>prostate cancer cells</topic><topic>Prostatic Neoplasms</topic><topic>Receptor, IGF Type 1 - analysis</topic><topic>Receptor, IGF Type 1 - genetics</topic><topic>Receptor, IGF Type 1 - metabolism</topic><topic>Receptor, IGF Type 2 - analysis</topic><topic>Receptor, IGF Type 2 - genetics</topic><topic>Receptor, IGF Type 2 - metabolism</topic><topic>RNA, Messenger - analysis</topic><topic>Tumor Cells, Cultured - chemistry</topic><topic>Tumor Cells, Cultured - cytology</topic><topic>Tumor Cells, Cultured - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kimura, Go</creatorcontrib><creatorcontrib>Kasuya, Junko</creatorcontrib><creatorcontrib>Giannini, Stefano</creatorcontrib><creatorcontrib>Honda, Yoko</creatorcontrib><creatorcontrib>Mohan, Subburaman</creatorcontrib><creatorcontrib>Kawachi, Mark H.</creatorcontrib><creatorcontrib>Akimoto, Masato</creatorcontrib><creatorcontrib>Fujita-Yamaguchi, Yoko</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of urology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kimura, Go</au><au>Kasuya, Junko</au><au>Giannini, Stefano</au><au>Honda, Yoko</au><au>Mohan, Subburaman</au><au>Kawachi, Mark H.</au><au>Akimoto, Masato</au><au>Fujita-Yamaguchi, Yoko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insulin-Like Growth Factor (IGF) System Components in Human Prostatic Cancer Cell-Lines: LNCaP, DU145, and PC-3 Cells</atitle><jtitle>International journal of urology</jtitle><addtitle>Int J Urol</addtitle><date>1996-01</date><risdate>1996</risdate><volume>3</volume><issue>1</issue><spage>39</spage><epage>46</epage><pages>39-46</pages><issn>0919-8172</issn><eissn>1442-2042</eissn><abstract>Background: Evidence has been accumulating that in many tumors, insulin‐like growth factors (ICFs) promote cancer cell growth in an autocrine/paracrine manner via the IGF‐1 receptor. In an effort to understand the role of ICFs in prostate cancer cell growth, we characterized the IGF system components produced by human prostatic cancer cell‐lines, LNCaP, DU145, and PC‐3, grown in serum‐free medium.
Methods: IGFs, their receptors, and IGF binding proteins (IGFBPs) produced by the three human prostate cell lines were characterized by reverse transcriptase‐polymerase chain reaction (RT‐PCR), radioimmunoassay (RIA), Western ligand blot, Western immunoblot, and Northern blot analyses.
Results: mRNA for IGF‐II and receptors for IGF‐I and IGF‐II were detected in all three cell‐lines by RT‐PCR. In contrast to the published study, only LNCaP cells expressed a trace amount of IGF‐I mRNA. RIA on conditioned media collected from these cells revealed that all three cell‐lines produced measurable IGF‐II but not IGF‐I. Western ligand blot, Western immunoblot, and Northern blot analyses revealed that LNCaP, DU145, and PC‐3 cells expressed IGFBP‐2, IGFBP‐2/‐3/‐4/‐6, and IGFBP‐2/‐3/‐4/‐5/‐6, respectively. IGF‐II stimulated [3H]thymidine incorporation into DNA in DU145 and PC‐3 cells significantly although the effect was small. DNA synthesis in PC‐3 cells but not in LNCaP and DU145 cells was significantly inhibited by the IGF‐I receptor‐specific monoclonal antibody.
Conclusion: These results suggest potentially important roles of IGFs and IGFBPs in prostate cancer cell growth, and that in particular, IGF‐II may play a critical role in prostate cancer cell growth.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8646598</pmid><doi>10.1111/j.1442-2042.1996.tb00628.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0919-8172 |
| ispartof | International journal of urology, 1996-01, Vol.3 (1), p.39-46 |
| issn | 0919-8172 1442-2042 |
| language | eng |
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| subjects | Antibodies, Monoclonal Base Sequence Blotting, Northern Cell Division - drug effects Cell Division - immunology cell growth Culture Media, Conditioned - chemistry Humans IGF-I receptor monoclonal antibody IGFBPs IGFs Insulin-Like Growth Factor Binding Protein 3 - analysis Insulin-Like Growth Factor Binding Protein 3 - genetics Insulin-Like Growth Factor Binding Protein 4 - genetics Insulin-Like Growth Factor Binding Protein 4 - metabolism Insulin-Like Growth Factor Binding Protein 5 - genetics Insulin-Like Growth Factor Binding Protein 5 - metabolism Insulin-Like Growth Factor Binding Protein 6 - genetics Insulin-Like Growth Factor Binding Protein 6 - metabolism Insulin-Like Growth Factor Binding Proteins - analysis Insulin-Like Growth Factor Binding Proteins - genetics Insulin-Like Growth Factor Binding Proteins - secretion Insulin-Like Growth Factor I - analysis Insulin-Like Growth Factor I - genetics Insulin-Like Growth Factor II - analysis Insulin-Like Growth Factor II - genetics Insulin-Like Growth Factor II - pharmacology Male Molecular Sequence Data prostate cancer cells Prostatic Neoplasms Receptor, IGF Type 1 - analysis Receptor, IGF Type 1 - genetics Receptor, IGF Type 1 - metabolism Receptor, IGF Type 2 - analysis Receptor, IGF Type 2 - genetics Receptor, IGF Type 2 - metabolism RNA, Messenger - analysis Tumor Cells, Cultured - chemistry Tumor Cells, Cultured - cytology Tumor Cells, Cultured - physiology |
| title | Insulin-Like Growth Factor (IGF) System Components in Human Prostatic Cancer Cell-Lines: LNCaP, DU145, and PC-3 Cells |
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