Regulation of ICAM-1 expression by inflammatory cytokines in rat mesangial cells
We investigated the regulation of intercellular adhesion molecule 1 (ICAM-1) expression by the inflammatory cytokines interleukin 1β (IL-1), IL-6 and IL-8 in rat mesangial cells by enzyme-linked immunosorbent assay, flow cytometry and Northern blot analyses. ICAM-1 expression on mesangial cells was...
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| Veröffentlicht in: | Cytokine (Philadelphia, Pa.) Pa.), 1996-02, Vol.8 (2), p.109-114 |
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| container_end_page | 114 |
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| container_title | Cytokine (Philadelphia, Pa.) |
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| creator | Ikeda, Michiyo Ikeda, Michiyo Ikeda, Uichi Shimada, Kazuyuki Minota, Seiji Kano, Shogo |
| description | We investigated the regulation of intercellular adhesion molecule 1 (ICAM-1) expression by the inflammatory cytokines interleukin 1β (IL-1), IL-6 and IL-8 in rat mesangial cells by enzyme-linked immunosorbent assay, flow cytometry and Northern blot analyses. ICAM-1 expression on mesangial cells was stimulated significantly by IL-1β, but not by IL-6 nor IL-8, in a dose-dependent manner. Levels of ICAM-1 mRNA were very low in unstimulated mesangial cells, while its expression was markedly induced by exposure to IL-1β for 3 h. IL-6 and IL-8 showed no effect on ICAM-1 mRNA accumulation. These results demonstrate that IL-1β, but not IL-6 nor IL-8, induces ICAM-1 mRNA and protein accumulation in rat mesangial cells. |
| doi_str_mv | 10.1006/cyto.1996.0015 |
| format | Article |
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ICAM-1 expression on mesangial cells was stimulated significantly by IL-1β, but not by IL-6 nor IL-8, in a dose-dependent manner. Levels of ICAM-1 mRNA were very low in unstimulated mesangial cells, while its expression was markedly induced by exposure to IL-1β for 3 h. IL-6 and IL-8 showed no effect on ICAM-1 mRNA accumulation. 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ICAM-1 expression on mesangial cells was stimulated significantly by IL-1β, but not by IL-6 nor IL-8, in a dose-dependent manner. Levels of ICAM-1 mRNA were very low in unstimulated mesangial cells, while its expression was markedly induced by exposure to IL-1β for 3 h. IL-6 and IL-8 showed no effect on ICAM-1 mRNA accumulation. These results demonstrate that IL-1β, but not IL-6 nor IL-8, induces ICAM-1 mRNA and protein accumulation in rat mesangial cells.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Glomerular Mesangium - cytology</subject><subject>Glomerular Mesangium - drug effects</subject><subject>Glomerular Mesangium - metabolism</subject><subject>glomerulonephritis</subject><subject>Intercellular Adhesion Molecule-1 - biosynthesis</subject><subject>Intercellular Adhesion Molecule-1 - genetics</subject><subject>interleukin-1</subject><subject>Interleukin-1 - pharmacology</subject><subject>interleukin-6</subject><subject>Interleukin-6 - pharmacology</subject><subject>interleukin-8</subject><subject>Interleukin-8 - pharmacology</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Recombinant Proteins - pharmacology</subject><subject>RNA, Messenger - biosynthesis</subject><issn>1043-4666</issn><issn>1096-0023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UE1LxDAQDaKsunr1JuTkrXXSj6Q9yuIXrCii55AmkyXajzVpxf57W3bx5mmGmTfvvXmEXDCIGQC_1mPfxawseQzA8gNywqDkEUCSHs59lkYZ5_yYnIbwAQBlKsSCLAohRMLFCXl5xc1Qq951Le0sfVzdPEWM4s_WYwjzsBqpa22tmkb1nR_prPfpWgzTmHrV0waDajdO1VRjXYczcmRVHfB8X5fk_e72bfUQrZ_vJ_Z1pDPI-8iAKMpk8lNylaHOE2ETITKGIjWCc2tThJQZMImtWJFYba0xSqjKGmBCs3RJrna8W999DRh62bgwO1AtdkOQooA8nRQmYLwDat-F4NHKrXeN8qNkIOcI5fySnCOUc4TTweWeeagaNH_wfWbTvtjtcXrv26GXQTtsNRrnUffSdO4_6l_ln4Cg</recordid><startdate>19960201</startdate><enddate>19960201</enddate><creator>Ikeda, Michiyo</creator><creator>Ikeda, Michiyo</creator><creator>Ikeda, Uichi</creator><creator>Shimada, Kazuyuki</creator><creator>Minota, Seiji</creator><creator>Kano, Shogo</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960201</creationdate><title>Regulation of ICAM-1 expression by inflammatory cytokines in rat mesangial cells</title><author>Ikeda, Michiyo ; Ikeda, Michiyo ; Ikeda, Uichi ; Shimada, Kazuyuki ; Minota, Seiji ; Kano, Shogo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-d0789200996a4ec527f27741e73d766ff3e031d0d2fb182fcffdda7abfd017c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Glomerular Mesangium - cytology</topic><topic>Glomerular Mesangium - drug effects</topic><topic>Glomerular Mesangium - metabolism</topic><topic>glomerulonephritis</topic><topic>Intercellular Adhesion Molecule-1 - biosynthesis</topic><topic>Intercellular Adhesion Molecule-1 - genetics</topic><topic>interleukin-1</topic><topic>Interleukin-1 - pharmacology</topic><topic>interleukin-6</topic><topic>Interleukin-6 - pharmacology</topic><topic>interleukin-8</topic><topic>Interleukin-8 - pharmacology</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Recombinant Proteins - pharmacology</topic><topic>RNA, Messenger - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ikeda, Michiyo</creatorcontrib><creatorcontrib>Ikeda, Michiyo</creatorcontrib><creatorcontrib>Ikeda, Uichi</creatorcontrib><creatorcontrib>Shimada, Kazuyuki</creatorcontrib><creatorcontrib>Minota, Seiji</creatorcontrib><creatorcontrib>Kano, Shogo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytokine (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ikeda, Michiyo</au><au>Ikeda, Michiyo</au><au>Ikeda, Uichi</au><au>Shimada, Kazuyuki</au><au>Minota, Seiji</au><au>Kano, Shogo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of ICAM-1 expression by inflammatory cytokines in rat mesangial cells</atitle><jtitle>Cytokine (Philadelphia, Pa.)</jtitle><addtitle>Cytokine</addtitle><date>1996-02-01</date><risdate>1996</risdate><volume>8</volume><issue>2</issue><spage>109</spage><epage>114</epage><pages>109-114</pages><issn>1043-4666</issn><eissn>1096-0023</eissn><abstract>We investigated the regulation of intercellular adhesion molecule 1 (ICAM-1) expression by the inflammatory cytokines interleukin 1β (IL-1), IL-6 and IL-8 in rat mesangial cells by enzyme-linked immunosorbent assay, flow cytometry and Northern blot analyses. ICAM-1 expression on mesangial cells was stimulated significantly by IL-1β, but not by IL-6 nor IL-8, in a dose-dependent manner. Levels of ICAM-1 mRNA were very low in unstimulated mesangial cells, while its expression was markedly induced by exposure to IL-1β for 3 h. IL-6 and IL-8 showed no effect on ICAM-1 mRNA accumulation. These results demonstrate that IL-1β, but not IL-6 nor IL-8, induces ICAM-1 mRNA and protein accumulation in rat mesangial cells.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>8777267</pmid><doi>10.1006/cyto.1996.0015</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 1043-4666 |
| ispartof | Cytokine (Philadelphia, Pa.), 1996-02, Vol.8 (2), p.109-114 |
| issn | 1043-4666 1096-0023 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cells, Cultured Glomerular Mesangium - cytology Glomerular Mesangium - drug effects Glomerular Mesangium - metabolism glomerulonephritis Intercellular Adhesion Molecule-1 - biosynthesis Intercellular Adhesion Molecule-1 - genetics interleukin-1 Interleukin-1 - pharmacology interleukin-6 Interleukin-6 - pharmacology interleukin-8 Interleukin-8 - pharmacology Male Rats Rats, Sprague-Dawley Recombinant Proteins - pharmacology RNA, Messenger - biosynthesis |
| title | Regulation of ICAM-1 expression by inflammatory cytokines in rat mesangial cells |
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