Actinic light density dependence of the O intermediate of the photocycle of bacteriorhodopsin
The O intermediate of the photocycle of bacteriorhodopsin (BR) was studied by absorption kinetic measurements at different actinic light densities. With increasing exciting flash intensity, the relative yield of O slightly increases, while that of M f strongly decreases at the expense of M s. Kineti...
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| Veröffentlicht in: | FEBS letters 1996-05, Vol.386 (1), p.55-59 |
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| container_title | FEBS letters |
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| creator | Luchian, Tudor Tokaji, Zsolt Dancsházy, Zsolt |
| description | The O intermediate of the photocycle of bacteriorhodopsin (BR) was studied by absorption kinetic measurements at different actinic light densities. With increasing exciting flash intensity, the relative yield of O slightly increases, while that of M
f strongly decreases at the expense of M
s. Kinetic calculations and the optical anisotropy of O show that O can be formed only from M
f although M
f and O have different light intensity dependences. In order to resolve the apparent contradiction, a phenomenologically new cooperative regulatory mechanism seems to be necessary. |
| doi_str_mv | 10.1016/0014-5793(96)00391-2 |
| format | Article |
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f strongly decreases at the expense of M
s. Kinetic calculations and the optical anisotropy of O show that O can be formed only from M
f although M
f and O have different light intensity dependences. In order to resolve the apparent contradiction, a phenomenologically new cooperative regulatory mechanism seems to be necessary.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(96)00391-2</identifier><identifier>PMID: 8635604</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Absorption ; bacteriorhodopsin ; Bacteriorhodopsins - physiology ; Bacteriorhodopsins - radiation effects ; Halobacterium halobium ; intermediates of the photocycle ; Kinetics ; Light ; Light intensity dependence ; M intermediate decay ; Mf, Ms, N and O ; Models, Biological ; Oxygen - metabolism ; Parallel pathways ; Photochemistry ; Photocooperativity ; Photocycle model ; Time Factors</subject><ispartof>FEBS letters, 1996-05, Vol.386 (1), p.55-59</ispartof><rights>1996</rights><rights>FEBS Letters 386 (1996) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4972-cec7f042cbdb59f67addbfb5b8d76eebde0c7ba5b7a049ccda27f096fcf912b33</citedby><cites>FETCH-LOGICAL-c4972-cec7f042cbdb59f67addbfb5b8d76eebde0c7ba5b7a049ccda27f096fcf912b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2F0014-5793%2896%2900391-2$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(96)00391-2$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,3548,27923,27924,45573,45574,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8635604$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luchian, Tudor</creatorcontrib><creatorcontrib>Tokaji, Zsolt</creatorcontrib><creatorcontrib>Dancsházy, Zsolt</creatorcontrib><title>Actinic light density dependence of the O intermediate of the photocycle of bacteriorhodopsin</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The O intermediate of the photocycle of bacteriorhodopsin (BR) was studied by absorption kinetic measurements at different actinic light densities. With increasing exciting flash intensity, the relative yield of O slightly increases, while that of M
f strongly decreases at the expense of M
s. Kinetic calculations and the optical anisotropy of O show that O can be formed only from M
f although M
f and O have different light intensity dependences. In order to resolve the apparent contradiction, a phenomenologically new cooperative regulatory mechanism seems to be necessary.</description><subject>Absorption</subject><subject>bacteriorhodopsin</subject><subject>Bacteriorhodopsins - physiology</subject><subject>Bacteriorhodopsins - radiation effects</subject><subject>Halobacterium halobium</subject><subject>intermediates of the photocycle</subject><subject>Kinetics</subject><subject>Light</subject><subject>Light intensity dependence</subject><subject>M intermediate decay</subject><subject>Mf, Ms, N and O</subject><subject>Models, Biological</subject><subject>Oxygen - metabolism</subject><subject>Parallel pathways</subject><subject>Photochemistry</subject><subject>Photocooperativity</subject><subject>Photocycle model</subject><subject>Time Factors</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS1EVZbCPwApJwSHtLaT2PEFqa26gFSpl3JElj2esEbZONhe0P57nO5qj8BpNG_evLE-E_KG0UtGmbiilLV1J1XzXokPlDaK1fwZWbFeNnXTiv45WZ0sL8jLlH7Q0vdMnZPzXjSdoO2KfLuG7CcP1ei_b3LlcEo-70udcSoNYBWGKm-weqj8lDFu0XmTT-q8CTnAHsYnxRooFh_iJrgwJz-9ImeDGRO-PtYL8nV993j7ub5_-PTl9vq-hlZJXgOCHGjLwTrbqUFI45wdbGd7JwWidUhBWtNZaWirAJzhxa_EAINi3DbNBXl3yJ1j-LnDlPXWJ8BxNBOGXdKypx3n9N9G1olGCi6KsT0YIYaUIg56jn5r4l4zqhf8emGrF7ZaLU3Br3lZe3vM39mC6rR05F3m68P8tx9x_1-Zen13w5fBoivxpC6HPh6CsGD95THqBH75L-cjQtYu-L-_9A8QWKms</recordid><startdate>19960513</startdate><enddate>19960513</enddate><creator>Luchian, Tudor</creator><creator>Tokaji, Zsolt</creator><creator>Dancsházy, Zsolt</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19960513</creationdate><title>Actinic light density dependence of the O intermediate of the photocycle of bacteriorhodopsin</title><author>Luchian, Tudor ; Tokaji, Zsolt ; Dancsházy, Zsolt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4972-cec7f042cbdb59f67addbfb5b8d76eebde0c7ba5b7a049ccda27f096fcf912b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Absorption</topic><topic>bacteriorhodopsin</topic><topic>Bacteriorhodopsins - physiology</topic><topic>Bacteriorhodopsins - radiation effects</topic><topic>Halobacterium halobium</topic><topic>intermediates of the photocycle</topic><topic>Kinetics</topic><topic>Light</topic><topic>Light intensity dependence</topic><topic>M intermediate decay</topic><topic>Mf, Ms, N and O</topic><topic>Models, Biological</topic><topic>Oxygen - metabolism</topic><topic>Parallel pathways</topic><topic>Photochemistry</topic><topic>Photocooperativity</topic><topic>Photocycle model</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luchian, Tudor</creatorcontrib><creatorcontrib>Tokaji, Zsolt</creatorcontrib><creatorcontrib>Dancsházy, Zsolt</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luchian, Tudor</au><au>Tokaji, Zsolt</au><au>Dancsházy, Zsolt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Actinic light density dependence of the O intermediate of the photocycle of bacteriorhodopsin</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1996-05-13</date><risdate>1996</risdate><volume>386</volume><issue>1</issue><spage>55</spage><epage>59</epage><pages>55-59</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The O intermediate of the photocycle of bacteriorhodopsin (BR) was studied by absorption kinetic measurements at different actinic light densities. With increasing exciting flash intensity, the relative yield of O slightly increases, while that of M
f strongly decreases at the expense of M
s. Kinetic calculations and the optical anisotropy of O show that O can be formed only from M
f although M
f and O have different light intensity dependences. In order to resolve the apparent contradiction, a phenomenologically new cooperative regulatory mechanism seems to be necessary.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8635604</pmid><doi>10.1016/0014-5793(96)00391-2</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1996-05, Vol.386 (1), p.55-59 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Absorption bacteriorhodopsin Bacteriorhodopsins - physiology Bacteriorhodopsins - radiation effects Halobacterium halobium intermediates of the photocycle Kinetics Light Light intensity dependence M intermediate decay Mf, Ms, N and O Models, Biological Oxygen - metabolism Parallel pathways Photochemistry Photocooperativity Photocycle model Time Factors |
| title | Actinic light density dependence of the O intermediate of the photocycle of bacteriorhodopsin |
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