Accumulation of Phosphorylated Neurofilaments in Anterior Horn Motoneurons of Amyotrophic Lateral Sclerosis Patients
Perikaryal collections of intermediate filaments have been described in the anterior horn motoneurons of patients with amyotrophic lateral sclerosis (ALS), but these inclusions have generally been considered rare and mainly associated with the familial form of ALS. Using the monoclonal antibody NF2F...
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| Veröffentlicht in: | Journal of neuropathology and experimental neurology 1988-01, Vol.47 (1), p.9-18 |
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| container_title | Journal of neuropathology and experimental neurology |
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| creator | Munoz, D G Greene, C Perl, D P Selkoe, D J |
| description | Perikaryal collections of intermediate filaments have been described in the anterior horn motoneurons of patients with amyotrophic lateral sclerosis (ALS), but these inclusions have generally been considered rare and mainly associated with the familial form of ALS. Using the monoclonal antibody NF2F11, which recognizes phosphorylated neurofilament epitopes, we showed that focal collections of neurofilaments in anterior horn motoneurons were a characteristic finding in sporadic as well as in familial ALS; they were present in seven of nine ALS patients, but in none of nine control spinal cords. These neurofilamentous collections are not cross-reactive with antibodies directed against paired helical filaments and the microtubule associated protein tau. In addition, diffuse staining for phosphorylated neurofilament epitopes in chromatolytic anterior horn perikarya was significantly more frequent in ALS patients than in controls. |
| doi_str_mv | 10.1097/00005072-198801000-00002 |
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Using the monoclonal antibody NF2F11, which recognizes phosphorylated neurofilament epitopes, we showed that focal collections of neurofilaments in anterior horn motoneurons were a characteristic finding in sporadic as well as in familial ALS; they were present in seven of nine ALS patients, but in none of nine control spinal cords. These neurofilamentous collections are not cross-reactive with antibodies directed against paired helical filaments and the microtubule associated protein tau. In addition, diffuse staining for phosphorylated neurofilament epitopes in chromatolytic anterior horn perikarya was significantly more frequent in ALS patients than in controls.</description><identifier>ISSN: 0022-3069</identifier><identifier>EISSN: 1554-6578</identifier><identifier>DOI: 10.1097/00005072-198801000-00002</identifier><identifier>PMID: 3334727</identifier><identifier>CODEN: JNENAD</identifier><language>eng</language><publisher>Hagerstown, MD: American Association of Neuropathologists, Inc</publisher><subject>Adult ; Aged ; Amyotrophic Lateral Sclerosis - genetics ; Amyotrophic Lateral Sclerosis - metabolism ; Amyotrophic Lateral Sclerosis - pathology ; Anterior Horn Cells - immunology ; Anterior Horn Cells - metabolism ; Anterior Horn Cells - pathology ; Antibodies, Monoclonal - immunology ; Biological and medical sciences ; Cerebellum - immunology ; Cerebellum - metabolism ; Cerebellum - pathology ; Cytoskeleton - pathology ; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases ; Female ; Humans ; Immunohistochemistry ; Intermediate Filaments - immunology ; Intermediate Filaments - metabolism ; Intermediate Filaments - pathology ; Male ; Medical sciences ; Middle Aged ; Motor Neurons - pathology ; Neurology ; Phosphorylation ; Spinal Cord - immunology ; Spinal Cord - metabolism ; Spinal Cord - pathology</subject><ispartof>Journal of neuropathology and experimental neurology, 1988-01, Vol.47 (1), p.9-18</ispartof><rights>1988 American Association of Neuropathologists, Inc</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4502-f46877475401e01e9c0cae581397775194735ed1c9ca52c982df2fac47c6eaa43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7721569$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3334727$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Munoz, D G</creatorcontrib><creatorcontrib>Greene, C</creatorcontrib><creatorcontrib>Perl, D P</creatorcontrib><creatorcontrib>Selkoe, D J</creatorcontrib><title>Accumulation of Phosphorylated Neurofilaments in Anterior Horn Motoneurons of Amyotrophic Lateral Sclerosis Patients</title><title>Journal of neuropathology and experimental neurology</title><addtitle>J Neuropathol Exp Neurol</addtitle><description>Perikaryal collections of intermediate filaments have been described in the anterior horn motoneurons of patients with amyotrophic lateral sclerosis (ALS), but these inclusions have generally been considered rare and mainly associated with the familial form of ALS. Using the monoclonal antibody NF2F11, which recognizes phosphorylated neurofilament epitopes, we showed that focal collections of neurofilaments in anterior horn motoneurons were a characteristic finding in sporadic as well as in familial ALS; they were present in seven of nine ALS patients, but in none of nine control spinal cords. These neurofilamentous collections are not cross-reactive with antibodies directed against paired helical filaments and the microtubule associated protein tau. In addition, diffuse staining for phosphorylated neurofilament epitopes in chromatolytic anterior horn perikarya was significantly more frequent in ALS patients than in controls.</description><subject>Adult</subject><subject>Aged</subject><subject>Amyotrophic Lateral Sclerosis - genetics</subject><subject>Amyotrophic Lateral Sclerosis - metabolism</subject><subject>Amyotrophic Lateral Sclerosis - pathology</subject><subject>Anterior Horn Cells - immunology</subject><subject>Anterior Horn Cells - metabolism</subject><subject>Anterior Horn Cells - pathology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biological and medical sciences</subject><subject>Cerebellum - immunology</subject><subject>Cerebellum - metabolism</subject><subject>Cerebellum - pathology</subject><subject>Cytoskeleton - pathology</subject><subject>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</subject><subject>Female</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Intermediate Filaments - immunology</subject><subject>Intermediate Filaments - metabolism</subject><subject>Intermediate Filaments - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Motor Neurons - pathology</subject><subject>Neurology</subject><subject>Phosphorylation</subject><subject>Spinal Cord - immunology</subject><subject>Spinal Cord - metabolism</subject><subject>Spinal Cord - pathology</subject><issn>0022-3069</issn><issn>1554-6578</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kVFv2yAUhdHUqs26_oRJPEx78wYYjHmMqq2tlK6R2j0jSq5lVgwZ2Iry74eXNG9FSIhzv3MvOiCEKflGiZLfSVmCSFZR1baElls1S-wDWlAheNUI2Z6hRVFYVZNGXaKPOf8phCKKX6CLuq65ZHKBxqW10zB5M7oYcOzwuo9528e0LxJs8C-YUuycNwOEMWMX8DKMkFxM-C6mgB_iGMPMhDy7l8M-jilue2fxqjRIxuMn6yHF7DJelylzm0_ovDM-w_XxvEK_f_54vrmrVo-39zfLVWW5IKzqeNNKyaXghELZyhJrQLS0VlJKQRWXtYANtcoawaxq2aZjnbFc2gaM4fUV-nrou03x7wR51IPLFrw3AeKUtWwJJ6IhBWwPoC0PzQk6vU1uMGmvKdFz4PotcH0K_L_EivXzccb0MsDmZDwmXOpfjnWTrfFdMsG6fMKkZFQ0qmD8gO2iL7HlVz_tIOkejB97_d531_8AOyyZdA</recordid><startdate>198801</startdate><enddate>198801</enddate><creator>Munoz, D G</creator><creator>Greene, C</creator><creator>Perl, D P</creator><creator>Selkoe, D J</creator><general>American Association of Neuropathologists, Inc</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198801</creationdate><title>Accumulation of Phosphorylated Neurofilaments in Anterior Horn Motoneurons of Amyotrophic Lateral Sclerosis Patients</title><author>Munoz, D G ; Greene, C ; Perl, D P ; Selkoe, D J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4502-f46877475401e01e9c0cae581397775194735ed1c9ca52c982df2fac47c6eaa43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Amyotrophic Lateral Sclerosis - genetics</topic><topic>Amyotrophic Lateral Sclerosis - metabolism</topic><topic>Amyotrophic Lateral Sclerosis - pathology</topic><topic>Anterior Horn Cells - immunology</topic><topic>Anterior Horn Cells - metabolism</topic><topic>Anterior Horn Cells - pathology</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Biological and medical sciences</topic><topic>Cerebellum - immunology</topic><topic>Cerebellum - metabolism</topic><topic>Cerebellum - pathology</topic><topic>Cytoskeleton - pathology</topic><topic>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</topic><topic>Female</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Intermediate Filaments - immunology</topic><topic>Intermediate Filaments - metabolism</topic><topic>Intermediate Filaments - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Motor Neurons - pathology</topic><topic>Neurology</topic><topic>Phosphorylation</topic><topic>Spinal Cord - immunology</topic><topic>Spinal Cord - metabolism</topic><topic>Spinal Cord - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Munoz, D G</creatorcontrib><creatorcontrib>Greene, C</creatorcontrib><creatorcontrib>Perl, D P</creatorcontrib><creatorcontrib>Selkoe, D J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuropathology and experimental neurology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Munoz, D G</au><au>Greene, C</au><au>Perl, D P</au><au>Selkoe, D J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accumulation of Phosphorylated Neurofilaments in Anterior Horn Motoneurons of Amyotrophic Lateral Sclerosis Patients</atitle><jtitle>Journal of neuropathology and experimental neurology</jtitle><addtitle>J Neuropathol Exp Neurol</addtitle><date>1988-01</date><risdate>1988</risdate><volume>47</volume><issue>1</issue><spage>9</spage><epage>18</epage><pages>9-18</pages><issn>0022-3069</issn><eissn>1554-6578</eissn><coden>JNENAD</coden><abstract>Perikaryal collections of intermediate filaments have been described in the anterior horn motoneurons of patients with amyotrophic lateral sclerosis (ALS), but these inclusions have generally been considered rare and mainly associated with the familial form of ALS. Using the monoclonal antibody NF2F11, which recognizes phosphorylated neurofilament epitopes, we showed that focal collections of neurofilaments in anterior horn motoneurons were a characteristic finding in sporadic as well as in familial ALS; they were present in seven of nine ALS patients, but in none of nine control spinal cords. These neurofilamentous collections are not cross-reactive with antibodies directed against paired helical filaments and the microtubule associated protein tau. In addition, diffuse staining for phosphorylated neurofilament epitopes in chromatolytic anterior horn perikarya was significantly more frequent in ALS patients than in controls.</abstract><cop>Hagerstown, MD</cop><pub>American Association of Neuropathologists, Inc</pub><pmid>3334727</pmid><doi>10.1097/00005072-198801000-00002</doi><tpages>10</tpages></addata></record> |
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| source | Oxford University Press Journals Digital Archive legacy; Journals@Ovid Ovid Autoload; MEDLINE |
| subjects | Adult Aged Amyotrophic Lateral Sclerosis - genetics Amyotrophic Lateral Sclerosis - metabolism Amyotrophic Lateral Sclerosis - pathology Anterior Horn Cells - immunology Anterior Horn Cells - metabolism Anterior Horn Cells - pathology Antibodies, Monoclonal - immunology Biological and medical sciences Cerebellum - immunology Cerebellum - metabolism Cerebellum - pathology Cytoskeleton - pathology Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Female Humans Immunohistochemistry Intermediate Filaments - immunology Intermediate Filaments - metabolism Intermediate Filaments - pathology Male Medical sciences Middle Aged Motor Neurons - pathology Neurology Phosphorylation Spinal Cord - immunology Spinal Cord - metabolism Spinal Cord - pathology |
| title | Accumulation of Phosphorylated Neurofilaments in Anterior Horn Motoneurons of Amyotrophic Lateral Sclerosis Patients |
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