cis-Acting Elements within an RNA Coliphage Genome: Fold as You Please, but Fold You Must

Using an in vivocomplementation system, we conducted a mutational analysis of the bacteriophage Qβ readthrough cistron. In the Qβ cDNA-containing plasmid, pQβm100, we constructed six defined Qβ deletion cDNA genomes, each missing between 86 and 447 nucleotides from within the readthrough cistron. Th...

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Veröffentlicht in:	Journal of molecular biology 1996-05, Vol.258 (3), p.433-446
Hauptverfasser:	Arora, Ruchi, Priano, Christine, Jacobson, Ann B., Mills, Donald R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Coliphages - chemistry Coliphages - genetics Coliphages - pathogenicity Directed Molecular Evolution - methods DNA, Complementary - genetics Genetic Complementation Test Genome, Viral Molecular Sequence Data Nucleic Acid Conformation phage Q beta Phenotype Plasmids - genetics protein complementation Qβ RNA readthrough protein RNA bacteriophages RNA secondary structure RNA Viruses - chemistry RNA Viruses - genetics RNA Viruses - pathogenicity RNA, Viral - chemistry Sequence Analysis, DNA Sequence Deletion Serial Passage Viral Plaque Assay Viral Proteins - genetics Virus Assembly
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