Transplantation of cultured human adrenal chromaffin cells into 6-hydroxydopamine-lesioned rat brain
Adult young rats were subjected to a unilateral 6‐hydroxydopamine lesion of the nigrostriatal dopamine pathway and then given intrastriatal grafts of human fetal adrenal chromaffin cell cultures. Amphetamine‐induced ipsiversive turning behavior in the lesioned rats was largely reversed in four of ei...
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| Veröffentlicht in: | Synapse (New York, N.Y.) N.Y.), 1987, Vol.1 (4), p.324-328 |
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| creator | Kamo, Hisaki Kim, Seung U. McGeer, Patrick L. Tago, Hisao Shin, Doo H. |
| description | Adult young rats were subjected to a unilateral 6‐hydroxydopamine lesion of the nigrostriatal dopamine pathway and then given intrastriatal grafts of human fetal adrenal chromaffin cell cultures. Amphetamine‐induced ipsiversive turning behavior in the lesioned rats was largely reversed in four of eight rats given such transplants when tested at 1.5 and 4.5 months post‐transplantation. Two rats showed a transient recovery at 1.5 months followed by deterioration at 4.5 months, while two other rats showed continuous deterioration. Six rats given sciatic nerve grafts as controls all showed deterioration from the pretransplantation levels. Catecholamine fluorescent and immunohistochemical examination of chromaffin‐cell‐transplanted brains demonstrated neurons and neuronal processes positive for catecholamines or tyrosine hydroxylase in the transplanted area. This transplantation of cultured human fetal cells to an animal model may provide the necessary basic experimental system for assessing the possible utility of human neuronal transplants. |
| doi_str_mv | 10.1002/syn.890010405 |
| format | Article |
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Amphetamine‐induced ipsiversive turning behavior in the lesioned rats was largely reversed in four of eight rats given such transplants when tested at 1.5 and 4.5 months post‐transplantation. Two rats showed a transient recovery at 1.5 months followed by deterioration at 4.5 months, while two other rats showed continuous deterioration. Six rats given sciatic nerve grafts as controls all showed deterioration from the pretransplantation levels. Catecholamine fluorescent and immunohistochemical examination of chromaffin‐cell‐transplanted brains demonstrated neurons and neuronal processes positive for catecholamines or tyrosine hydroxylase in the transplanted area. This transplantation of cultured human fetal cells to an animal model may provide the necessary basic experimental system for assessing the possible utility of human neuronal transplants.</description><identifier>ISSN: 0887-4476</identifier><identifier>EISSN: 1098-2396</identifier><identifier>DOI: 10.1002/syn.890010405</identifier><identifier>PMID: 2901789</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adrenal medulla ; Adrenal Medulla - cytology ; Adrenal Medulla - embryology ; Adrenal Medulla - transplantation ; Amphetamines - pharmacology ; Animals ; Brain transplant ; Catecholamines - metabolism ; Cells, Cultured ; Corpus Striatum - drug effects ; Corpus Striatum - metabolism ; Corpus Striatum - physiology ; Cultured cells ; Gestational Age ; Graft Survival ; Human fetal ; Humans ; Hydroxydopamines ; Male ; Oxidopamine ; Rats ; Rats, Inbred Strains ; Stereotyped Behavior - drug effects ; Stereotyped Behavior - physiology ; Substantia Nigra - drug effects ; Substantia Nigra - metabolism ; Substantia Nigra - physiology ; Tyrosine 3-Monooxygenase - metabolism</subject><ispartof>Synapse (New York, N.Y.), 1987, Vol.1 (4), p.324-328</ispartof><rights>Copyright © 1987 Alan R. Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4055-dcc7fdaf134fd28a925ea836873f0ce6855c1fbf5fcfb1a026183858ed67ab763</citedby><cites>FETCH-LOGICAL-c4055-dcc7fdaf134fd28a925ea836873f0ce6855c1fbf5fcfb1a026183858ed67ab763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fsyn.890010405$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fsyn.890010405$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4023,27922,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2901789$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kamo, Hisaki</creatorcontrib><creatorcontrib>Kim, Seung U.</creatorcontrib><creatorcontrib>McGeer, Patrick L.</creatorcontrib><creatorcontrib>Tago, Hisao</creatorcontrib><creatorcontrib>Shin, Doo H.</creatorcontrib><title>Transplantation of cultured human adrenal chromaffin cells into 6-hydroxydopamine-lesioned rat brain</title><title>Synapse (New York, N.Y.)</title><addtitle>Synapse</addtitle><description>Adult young rats were subjected to a unilateral 6‐hydroxydopamine lesion of the nigrostriatal dopamine pathway and then given intrastriatal grafts of human fetal adrenal chromaffin cell cultures. Amphetamine‐induced ipsiversive turning behavior in the lesioned rats was largely reversed in four of eight rats given such transplants when tested at 1.5 and 4.5 months post‐transplantation. Two rats showed a transient recovery at 1.5 months followed by deterioration at 4.5 months, while two other rats showed continuous deterioration. Six rats given sciatic nerve grafts as controls all showed deterioration from the pretransplantation levels. Catecholamine fluorescent and immunohistochemical examination of chromaffin‐cell‐transplanted brains demonstrated neurons and neuronal processes positive for catecholamines or tyrosine hydroxylase in the transplanted area. This transplantation of cultured human fetal cells to an animal model may provide the necessary basic experimental system for assessing the possible utility of human neuronal transplants.</description><subject>Adrenal medulla</subject><subject>Adrenal Medulla - cytology</subject><subject>Adrenal Medulla - embryology</subject><subject>Adrenal Medulla - transplantation</subject><subject>Amphetamines - pharmacology</subject><subject>Animals</subject><subject>Brain transplant</subject><subject>Catecholamines - metabolism</subject><subject>Cells, Cultured</subject><subject>Corpus Striatum - drug effects</subject><subject>Corpus Striatum - metabolism</subject><subject>Corpus Striatum - physiology</subject><subject>Cultured cells</subject><subject>Gestational Age</subject><subject>Graft Survival</subject><subject>Human fetal</subject><subject>Humans</subject><subject>Hydroxydopamines</subject><subject>Male</subject><subject>Oxidopamine</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Stereotyped Behavior - drug effects</subject><subject>Stereotyped Behavior - physiology</subject><subject>Substantia Nigra - drug effects</subject><subject>Substantia Nigra - metabolism</subject><subject>Substantia Nigra - physiology</subject><subject>Tyrosine 3-Monooxygenase - metabolism</subject><issn>0887-4476</issn><issn>1098-2396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rFTEUhoMo9ba6dClk5W5qPmaSzFKKtkKpBa-Iq3AmH9zoTHJNZrDz7025l0tXugqH85wnLy9Cbyi5pISw92WNl6onhJKWdM_QhpJeNYz34jnaEKVk07ZSvETnpfwkhPCKnaEz1hMqVb9Bdpshlv0IcYY5pIiTx2YZ5yU7i3fLBBGDzS7CiM0upwm8DxEbN44FhzgnLJrdanN6WG3awxSia0ZXqqieZ5jxkCHEV-iFh7G418f3An379HF7ddPcfrn-fPXhtjE1etdYY6S34ClvvWUKetY5UFwoyT0xTqiuM9QPvvPGDxQIE1Rx1SlnhYRBCn6B3h28-5x-L67MegrlMStEl5aipSJcMfZ_kLayrd-pCjYH0ORUSnZe73OYIK-aEv1Yv67161P9lX97FC_D5OyJPvZd9_Kw_xNGt_5bpr_-uHtqPiYJZXYPp0vIv7SQXHb6-921vrlnkm_rcM__AhXGohg</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>Kamo, Hisaki</creator><creator>Kim, Seung U.</creator><creator>McGeer, Patrick L.</creator><creator>Tago, Hisao</creator><creator>Shin, Doo H.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Transplantation of cultured human adrenal chromaffin cells into 6-hydroxydopamine-lesioned rat brain</title><author>Kamo, Hisaki ; Kim, Seung U. ; McGeer, Patrick L. ; Tago, Hisao ; Shin, Doo H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4055-dcc7fdaf134fd28a925ea836873f0ce6855c1fbf5fcfb1a026183858ed67ab763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Adrenal medulla</topic><topic>Adrenal Medulla - cytology</topic><topic>Adrenal Medulla - embryology</topic><topic>Adrenal Medulla - transplantation</topic><topic>Amphetamines - pharmacology</topic><topic>Animals</topic><topic>Brain transplant</topic><topic>Catecholamines - metabolism</topic><topic>Cells, Cultured</topic><topic>Corpus Striatum - drug effects</topic><topic>Corpus Striatum - metabolism</topic><topic>Corpus Striatum - physiology</topic><topic>Cultured cells</topic><topic>Gestational Age</topic><topic>Graft Survival</topic><topic>Human fetal</topic><topic>Humans</topic><topic>Hydroxydopamines</topic><topic>Male</topic><topic>Oxidopamine</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Stereotyped Behavior - drug effects</topic><topic>Stereotyped Behavior - physiology</topic><topic>Substantia Nigra - drug effects</topic><topic>Substantia Nigra - metabolism</topic><topic>Substantia Nigra - physiology</topic><topic>Tyrosine 3-Monooxygenase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kamo, Hisaki</creatorcontrib><creatorcontrib>Kim, Seung U.</creatorcontrib><creatorcontrib>McGeer, Patrick L.</creatorcontrib><creatorcontrib>Tago, Hisao</creatorcontrib><creatorcontrib>Shin, Doo H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Synapse (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kamo, Hisaki</au><au>Kim, Seung U.</au><au>McGeer, Patrick L.</au><au>Tago, Hisao</au><au>Shin, Doo H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transplantation of cultured human adrenal chromaffin cells into 6-hydroxydopamine-lesioned rat brain</atitle><jtitle>Synapse (New York, N.Y.)</jtitle><addtitle>Synapse</addtitle><date>1987</date><risdate>1987</risdate><volume>1</volume><issue>4</issue><spage>324</spage><epage>328</epage><pages>324-328</pages><issn>0887-4476</issn><eissn>1098-2396</eissn><abstract>Adult young rats were subjected to a unilateral 6‐hydroxydopamine lesion of the nigrostriatal dopamine pathway and then given intrastriatal grafts of human fetal adrenal chromaffin cell cultures. Amphetamine‐induced ipsiversive turning behavior in the lesioned rats was largely reversed in four of eight rats given such transplants when tested at 1.5 and 4.5 months post‐transplantation. Two rats showed a transient recovery at 1.5 months followed by deterioration at 4.5 months, while two other rats showed continuous deterioration. Six rats given sciatic nerve grafts as controls all showed deterioration from the pretransplantation levels. Catecholamine fluorescent and immunohistochemical examination of chromaffin‐cell‐transplanted brains demonstrated neurons and neuronal processes positive for catecholamines or tyrosine hydroxylase in the transplanted area. This transplantation of cultured human fetal cells to an animal model may provide the necessary basic experimental system for assessing the possible utility of human neuronal transplants.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2901789</pmid><doi>10.1002/syn.890010405</doi><tpages>5</tpages></addata></record> |
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| ispartof | Synapse (New York, N.Y.), 1987, Vol.1 (4), p.324-328 |
| issn | 0887-4476 1098-2396 |
| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Adrenal medulla Adrenal Medulla - cytology Adrenal Medulla - embryology Adrenal Medulla - transplantation Amphetamines - pharmacology Animals Brain transplant Catecholamines - metabolism Cells, Cultured Corpus Striatum - drug effects Corpus Striatum - metabolism Corpus Striatum - physiology Cultured cells Gestational Age Graft Survival Human fetal Humans Hydroxydopamines Male Oxidopamine Rats Rats, Inbred Strains Stereotyped Behavior - drug effects Stereotyped Behavior - physiology Substantia Nigra - drug effects Substantia Nigra - metabolism Substantia Nigra - physiology Tyrosine 3-Monooxygenase - metabolism |
| title | Transplantation of cultured human adrenal chromaffin cells into 6-hydroxydopamine-lesioned rat brain |
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