Differential binding of dexamethasone to ammonium sulfate precipitates of human adipose tissue cytosols
Saturation analysis of the binding of [ 3H]dexamethasone ([ 3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [ 3H]DEX for binding to the ASPs in the foll...
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| Veröffentlicht in: | Steroids 1987-06, Vol.49 (6), p.507-522 |
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| creator | Miller, Lorraine K. Kral, John G. Strain, Gladys W. Zumoff, Barnett |
| description | Saturation analysis of the binding of [
3H]dexamethasone ([
3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [
3H]DEX for binding to the ASPs in the following sequence: dexamethasone (DEX) ≅ triamcinolone acetonide (TA) > progesterone (P) > estradiol (E2). The steroid specificity of the binder precipitated by AS was consistent with the specificities reported for glucocorticoid receptors in a number of systems. In order to investigate possible regional differences, glucocorticoid binding to ASPs derived from adipose tissues removed from two different sites in the same subject was quantitated. ASPs of human omental adipose tissue bound significantly more [
3H]DEX than did similar preparations of subcutaneous adipose tissue from the abdominal wall (116 ± 32 vs. 50 ± 22 fmol/mg protein; mean ±SD; p < 0.02). The findings are consistent with reports from other laboratories suggesting that intra-abdominal fat is more responsive to glucocorticoids than is subcutaneous adipose tissue. |
| doi_str_mv | 10.1016/0039-128X(87)90091-2 |
| format | Article |
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3H]dexamethasone ([
3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [
3H]DEX for binding to the ASPs in the following sequence: dexamethasone (DEX) ≅ triamcinolone acetonide (TA) > progesterone (P) > estradiol (E2). The steroid specificity of the binder precipitated by AS was consistent with the specificities reported for glucocorticoid receptors in a number of systems. In order to investigate possible regional differences, glucocorticoid binding to ASPs derived from adipose tissues removed from two different sites in the same subject was quantitated. ASPs of human omental adipose tissue bound significantly more [
3H]DEX than did similar preparations of subcutaneous adipose tissue from the abdominal wall (116 ± 32 vs. 50 ± 22 fmol/mg protein; mean ±SD; p < 0.02). The findings are consistent with reports from other laboratories suggesting that intra-abdominal fat is more responsive to glucocorticoids than is subcutaneous adipose tissue.</description><identifier>ISSN: 0039-128X</identifier><identifier>EISSN: 1878-5867</identifier><identifier>DOI: 10.1016/0039-128X(87)90091-2</identifier><identifier>PMID: 3453560</identifier><identifier>CODEN: STEDAM</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>16a ; 17-acetonide Progesterone ; 17a ; 17g-diol ; 20-dione Triamcinolone acetonide ; 20-dione-16 ; 21-tetra- hydroxy-pregna-l ; 21-trihydroxy-pregna-l ; 4-diene-3 ; 4-pregnen-3/20-dione Estradiol 1 ; 5-estratrien-3 ; 9-fIuoro-16a-methyl-ll6 ; 9-fluoro-116 ; Adipose Tissue - metabolism ; Ammonium Sulfate ; Binding, Competitive ; Biological and medical sciences ; Cell receptors ; Cell structures and functions ; Chemical Precipitation ; Cytosol - metabolism ; Dexamethasone ; Dexamethasone - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; In Vitro Techniques ; Molecular and cellular biology ; Receptors, Glucocorticoid - isolation & purification ; Receptors, Glucocorticoid - metabolism</subject><ispartof>Steroids, 1987-06, Vol.49 (6), p.507-522</ispartof><rights>1987</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-bee3e5dd0d6a352e3b21f35991f8060f94dc8695830fcaffb2f4135d8998dc623</citedby><cites>FETCH-LOGICAL-c301t-bee3e5dd0d6a352e3b21f35991f8060f94dc8695830fcaffb2f4135d8998dc623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0039-128X(87)90091-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7045087$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3453560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miller, Lorraine K.</creatorcontrib><creatorcontrib>Kral, John G.</creatorcontrib><creatorcontrib>Strain, Gladys W.</creatorcontrib><creatorcontrib>Zumoff, Barnett</creatorcontrib><title>Differential binding of dexamethasone to ammonium sulfate precipitates of human adipose tissue cytosols</title><title>Steroids</title><addtitle>Steroids</addtitle><description>Saturation analysis of the binding of [
3H]dexamethasone ([
3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [
3H]DEX for binding to the ASPs in the following sequence: dexamethasone (DEX) ≅ triamcinolone acetonide (TA) > progesterone (P) > estradiol (E2). The steroid specificity of the binder precipitated by AS was consistent with the specificities reported for glucocorticoid receptors in a number of systems. In order to investigate possible regional differences, glucocorticoid binding to ASPs derived from adipose tissues removed from two different sites in the same subject was quantitated. ASPs of human omental adipose tissue bound significantly more [
3H]DEX than did similar preparations of subcutaneous adipose tissue from the abdominal wall (116 ± 32 vs. 50 ± 22 fmol/mg protein; mean ±SD; p < 0.02). The findings are consistent with reports from other laboratories suggesting that intra-abdominal fat is more responsive to glucocorticoids than is subcutaneous adipose tissue.</description><subject>16a</subject><subject>17-acetonide Progesterone</subject><subject>17a</subject><subject>17g-diol</subject><subject>20-dione Triamcinolone acetonide</subject><subject>20-dione-16</subject><subject>21-tetra- hydroxy-pregna-l</subject><subject>21-trihydroxy-pregna-l</subject><subject>4-diene-3</subject><subject>4-pregnen-3/20-dione Estradiol 1</subject><subject>5-estratrien-3</subject><subject>9-fIuoro-16a-methyl-ll6</subject><subject>9-fluoro-116</subject><subject>Adipose Tissue - metabolism</subject><subject>Ammonium Sulfate</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>Chemical Precipitation</subject><subject>Cytosol - metabolism</subject><subject>Dexamethasone</subject><subject>Dexamethasone - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Molecular and cellular biology</subject><subject>Receptors, Glucocorticoid - isolation & purification</subject><subject>Receptors, Glucocorticoid - metabolism</subject><issn>0039-128X</issn><issn>1878-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF2L1TAQhoMo63H1Hyj0QkQvqpOmaZMbQdZPWPBGwbuQJpPdSJvUTiruv7f1HM6lVzMwz_syPIw95fCaA-_eAAhd80b9eKn6VxpA87q5xw5c9aqWquvvs8MZecgeEf0EgE7o5oJdiFYK2cGB3byPIeCCqUQ7VkNMPqabKofK4x87Ybm1lBNWJVd2mnKK61TROgZbsJoXdHGOZdtpT9yuk02V9XHOtCUi0YqVuyuZ8kiP2YNgR8Inp3nJvn_88O3qc3399dOXq3fXtRPASz0gCpTeg--skA2KoeFBSK15UNBB0K13qtNSCQjOhjA0oeVCeqW18q5rxCV7ceydl_xrRSpmiuRwHG3CvJLpFYheS7mB7RF0SyZaMJh5iZNd7gwHs_s1uzyzyzOqN__8mr3_2al_HSb059BJ6HZ_frpbcnYMi00u0hnroZWg-g17e8Rwc_E74mLIRUwOfdysFuNz_P8ffwFdVpkj</recordid><startdate>198706</startdate><enddate>198706</enddate><creator>Miller, Lorraine K.</creator><creator>Kral, John G.</creator><creator>Strain, Gladys W.</creator><creator>Zumoff, Barnett</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198706</creationdate><title>Differential binding of dexamethasone to ammonium sulfate precipitates of human adipose tissue cytosols</title><author>Miller, Lorraine K. ; Kral, John G. ; Strain, Gladys W. ; Zumoff, Barnett</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-bee3e5dd0d6a352e3b21f35991f8060f94dc8695830fcaffb2f4135d8998dc623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>16a</topic><topic>17-acetonide Progesterone</topic><topic>17a</topic><topic>17g-diol</topic><topic>20-dione Triamcinolone acetonide</topic><topic>20-dione-16</topic><topic>21-tetra- hydroxy-pregna-l</topic><topic>21-trihydroxy-pregna-l</topic><topic>4-diene-3</topic><topic>4-pregnen-3/20-dione Estradiol 1</topic><topic>5-estratrien-3</topic><topic>9-fIuoro-16a-methyl-ll6</topic><topic>9-fluoro-116</topic><topic>Adipose Tissue - metabolism</topic><topic>Ammonium Sulfate</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>Chemical Precipitation</topic><topic>Cytosol - metabolism</topic><topic>Dexamethasone</topic><topic>Dexamethasone - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Molecular and cellular biology</topic><topic>Receptors, Glucocorticoid - isolation & purification</topic><topic>Receptors, Glucocorticoid - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miller, Lorraine K.</creatorcontrib><creatorcontrib>Kral, John G.</creatorcontrib><creatorcontrib>Strain, Gladys W.</creatorcontrib><creatorcontrib>Zumoff, Barnett</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miller, Lorraine K.</au><au>Kral, John G.</au><au>Strain, Gladys W.</au><au>Zumoff, Barnett</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential binding of dexamethasone to ammonium sulfate precipitates of human adipose tissue cytosols</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>1987-06</date><risdate>1987</risdate><volume>49</volume><issue>6</issue><spage>507</spage><epage>522</epage><pages>507-522</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><coden>STEDAM</coden><abstract>Saturation analysis of the binding of [
3H]dexamethasone ([
3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [
3H]DEX for binding to the ASPs in the following sequence: dexamethasone (DEX) ≅ triamcinolone acetonide (TA) > progesterone (P) > estradiol (E2). The steroid specificity of the binder precipitated by AS was consistent with the specificities reported for glucocorticoid receptors in a number of systems. In order to investigate possible regional differences, glucocorticoid binding to ASPs derived from adipose tissues removed from two different sites in the same subject was quantitated. ASPs of human omental adipose tissue bound significantly more [
3H]DEX than did similar preparations of subcutaneous adipose tissue from the abdominal wall (116 ± 32 vs. 50 ± 22 fmol/mg protein; mean ±SD; p < 0.02). The findings are consistent with reports from other laboratories suggesting that intra-abdominal fat is more responsive to glucocorticoids than is subcutaneous adipose tissue.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>3453560</pmid><doi>10.1016/0039-128X(87)90091-2</doi><tpages>16</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Steroids, 1987-06, Vol.49 (6), p.507-522 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 16a 17-acetonide Progesterone 17a 17g-diol 20-dione Triamcinolone acetonide 20-dione-16 21-tetra- hydroxy-pregna-l 21-trihydroxy-pregna-l 4-diene-3 4-pregnen-3/20-dione Estradiol 1 5-estratrien-3 9-fIuoro-16a-methyl-ll6 9-fluoro-116 Adipose Tissue - metabolism Ammonium Sulfate Binding, Competitive Biological and medical sciences Cell receptors Cell structures and functions Chemical Precipitation Cytosol - metabolism Dexamethasone Dexamethasone - metabolism Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Molecular and cellular biology Receptors, Glucocorticoid - isolation & purification Receptors, Glucocorticoid - metabolism |
| title | Differential binding of dexamethasone to ammonium sulfate precipitates of human adipose tissue cytosols |
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