Isolation and Characterization of Tissue-Type Plasminogen Activator-Binding Proteoglycans From Human Umbilical Vein Endothelial Cells

We analyzed the tissue-type plasminogen activator (TPA)-binding proteoglycans (PGs) on human umbilical vein endothelial cells (HUVECs), which were metabolically labeled with [sup 35 S]Na2 SO4. Cell extracts were then prepared and subjected to affinity chromatography on diisopropyl fluorophosphate (D...

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Veröffentlicht in:	Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 1996-05, Vol.16 (5), p.665-672
Hauptverfasser:	Bohm, Thomas, Geiger, Margarethe, Binder, Bernd R
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Blood coagulation. Blood cells Cells, Cultured Chondroitin Sulfates - isolation & purification Chondroitin Sulfates - metabolism Electrophoresis, Polyacrylamide Gel Endothelium, Vascular - cytology Endothelium, Vascular - metabolism Fundamental and applied biological sciences. Psychology General aspects, investigation methods, hemostasis, fibrinolysis Glycosaminoglycans - isolation & purification Glycosaminoglycans - metabolism Heparitin Sulfate - isolation & purification Heparitin Sulfate - metabolism Humans Molecular and cellular biology Proteoglycans - isolation & purification Proteoglycans - metabolism Tissue Plasminogen Activator - metabolism Umbilical Veins - cytology Umbilical Veins - metabolism
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