Nitrogen Metabolism in Lignifying Pinus taeda Cell Cultures (∗)
The primary metabolic fate of phenylalanine, following its deamination in plants, is conscription of its carbon skeleton for lignin, suberin, flavonoid, and related metabolite formation. Since this accounts for ∼30-40% of all organic carbon, an effective means of recycling the liberated ammonium ion...
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| Veröffentlicht in: | The Journal of biological chemistry 1996-05, Vol.271 (21), p.12350-12355 |
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| container_title | The Journal of biological chemistry |
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| creator | van Heerden, Pieter S. Towers, G. H. Neil Lewis, Norman G. |
| description | The primary metabolic fate of phenylalanine, following its deamination in plants, is conscription of its carbon skeleton for lignin, suberin, flavonoid, and related metabolite formation. Since this accounts for ∼30-40% of all organic carbon, an effective means of recycling the liberated ammonium ion must be operative. In order to establish how this occurs, the uptake and metabolism of various 15N-labeled precursors (15N-Phe, 15NH4Cl, 15N-Gln, and 15N-Glu) in lignifying Pinus taeda cell cultures was investigated, using a combination of high performance liquid chromatography, 15N NMR, and gas chromatography-mass spectrometry analyses. It was found that the ammonium ion released during active phenylpropanoid metabolism was not made available for general amino acid/protein synthesis. Rather it was rapidly recycled back to regenerate phenylalanine, thereby providing an effective means of maintaining active phenylpropanoid metabolism with no additional nitrogen requirement. These results strongly suggest that, in lignifying cells, ammonium ion reassimilation is tightly compartmentalized. |
| doi_str_mv | 10.1074/jbc.271.21.12350 |
| format | Article |
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H. Neil ; Lewis, Norman G.</creator><creatorcontrib>van Heerden, Pieter S. ; Towers, G. H. Neil ; Lewis, Norman G. ; University of the Orange Free State, Bloemfontein, South Africa</creatorcontrib><description>The primary metabolic fate of phenylalanine, following its deamination in plants, is conscription of its carbon skeleton for lignin, suberin, flavonoid, and related metabolite formation. Since this accounts for ∼30-40% of all organic carbon, an effective means of recycling the liberated ammonium ion must be operative. In order to establish how this occurs, the uptake and metabolism of various 15N-labeled precursors (15N-Phe, 15NH4Cl, 15N-Gln, and 15N-Glu) in lignifying Pinus taeda cell cultures was investigated, using a combination of high performance liquid chromatography, 15N NMR, and gas chromatography-mass spectrometry analyses. It was found that the ammonium ion released during active phenylpropanoid metabolism was not made available for general amino acid/protein synthesis. Rather it was rapidly recycled back to regenerate phenylalanine, thereby providing an effective means of maintaining active phenylpropanoid metabolism with no additional nitrogen requirement. These results strongly suggest that, in lignifying cells, ammonium ion reassimilation is tightly compartmentalized.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.271.21.12350</identifier><identifier>PMID: 8647837</identifier><language>eng</language><publisher>Legacy CDMS: Elsevier Inc</publisher><subject>Amino Acids - metabolism ; ammonia ; ammoniac ; amoniaco ; biochemical pathways ; Cells, Cultured ; Chromatography, High Pressure Liquid ; fenilalanina ; Gas Chromatography-Mass Spectrometry ; Life Sciences (General) ; lignificacion ; lignification ; ligninas ; lignine ; lignins ; Magnetic Resonance Spectroscopy ; metabolisme de l' azote ; metabolismo del nitrogeno ; metabolite ; metabolites ; metabolitos ; Nitrogen - metabolism ; Nitrogen Isotopes ; phenylalanine ; Pinus taeda ; Space life sciences ; technique des traceurs ; tecnicas de trazadores ; tracer techniques ; via bioquimica del metabolismo ; voie biochimique du metabolisme</subject><ispartof>The Journal of biological chemistry, 1996-05, Vol.271 (21), p.12350-12355</ispartof><rights>1996 © 1996 ASBMB. 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Neil</creatorcontrib><creatorcontrib>Lewis, Norman G.</creatorcontrib><creatorcontrib>University of the Orange Free State, Bloemfontein, South Africa</creatorcontrib><title>Nitrogen Metabolism in Lignifying Pinus taeda Cell Cultures (∗)</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>The primary metabolic fate of phenylalanine, following its deamination in plants, is conscription of its carbon skeleton for lignin, suberin, flavonoid, and related metabolite formation. Since this accounts for ∼30-40% of all organic carbon, an effective means of recycling the liberated ammonium ion must be operative. In order to establish how this occurs, the uptake and metabolism of various 15N-labeled precursors (15N-Phe, 15NH4Cl, 15N-Gln, and 15N-Glu) in lignifying Pinus taeda cell cultures was investigated, using a combination of high performance liquid chromatography, 15N NMR, and gas chromatography-mass spectrometry analyses. It was found that the ammonium ion released during active phenylpropanoid metabolism was not made available for general amino acid/protein synthesis. Rather it was rapidly recycled back to regenerate phenylalanine, thereby providing an effective means of maintaining active phenylpropanoid metabolism with no additional nitrogen requirement. These results strongly suggest that, in lignifying cells, ammonium ion reassimilation is tightly compartmentalized.</description><subject>Amino Acids - metabolism</subject><subject>ammonia</subject><subject>ammoniac</subject><subject>amoniaco</subject><subject>biochemical pathways</subject><subject>Cells, Cultured</subject><subject>Chromatography, High Pressure Liquid</subject><subject>fenilalanina</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Life Sciences (General)</subject><subject>lignificacion</subject><subject>lignification</subject><subject>ligninas</subject><subject>lignine</subject><subject>lignins</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>metabolisme de l' azote</subject><subject>metabolismo del nitrogeno</subject><subject>metabolite</subject><subject>metabolites</subject><subject>metabolitos</subject><subject>Nitrogen - metabolism</subject><subject>Nitrogen Isotopes</subject><subject>phenylalanine</subject><subject>Pinus taeda</subject><subject>Space life sciences</subject><subject>technique des traceurs</subject><subject>tecnicas de trazadores</subject><subject>tracer techniques</subject><subject>via bioquimica del metabolismo</subject><subject>voie biochimique du metabolisme</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>CYI</sourceid><sourceid>EIF</sourceid><recordid>eNqFkMtu1DAUhi0EKsPAngWgLBCCRQZfYsdmV40orTRcJKjEznLsk4yrxGntBNQ34A14P56kLhmxQEJ448X_n8_HH0KPCd4QXFevLxq7oTXZULIhlHF8B60IlqxknHy9i1YYU1IqyuV99CClC5xPpcgROpKiqiWrV-j4g5_i2EEo3sNkmrH3aSh8KHa-C7699qErPvkwp2Iy4Eyxhb4vtnM_zRFS8fLXj5-vHqJ7rekTPDrca3R-8vbL9rTcfXx3tj3elbZSbCo55cZIRZlruCVGOCExw9SayriKNs4qrKSzruUWc8Wcki20jaJONAJDK9gavVi4l3G8miFNevDJ5n1MgHFOus48zjD5b5FwISQVPBfxUrRxTClCqy-jH0y81gTrW70669VZr6ZE_9abR54e2HMzgPszcPCZ8ydLHkwyOkwxaZqdY1Izmglr9HyJ977bf_cRdONHu4fh71eeLbXWjNp00Sd9_pkoVWPClcK3Lt4sBcjCv3mIOlkPwYLLTDtpN_p__-EGxp-mVQ</recordid><startdate>19960524</startdate><enddate>19960524</enddate><creator>van Heerden, Pieter S.</creator><creator>Towers, G. H. Neil</creator><creator>Lewis, Norman G.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CYE</scope><scope>CYI</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19960524</creationdate><title>Nitrogen Metabolism in Lignifying Pinus taeda Cell Cultures (∗)</title><author>van Heerden, Pieter S. ; Towers, G. H. Neil ; Lewis, Norman G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-525aa8923db5c1a6d680302ca4ad42bdc9098dcdf5c0593d98fefb92d6b60ef63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acids - metabolism</topic><topic>ammonia</topic><topic>ammoniac</topic><topic>amoniaco</topic><topic>biochemical pathways</topic><topic>Cells, Cultured</topic><topic>Chromatography, High Pressure Liquid</topic><topic>fenilalanina</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Life Sciences (General)</topic><topic>lignificacion</topic><topic>lignification</topic><topic>ligninas</topic><topic>lignine</topic><topic>lignins</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>metabolisme de l' azote</topic><topic>metabolismo del nitrogeno</topic><topic>metabolite</topic><topic>metabolites</topic><topic>metabolitos</topic><topic>Nitrogen - metabolism</topic><topic>Nitrogen Isotopes</topic><topic>phenylalanine</topic><topic>Pinus taeda</topic><topic>Space life sciences</topic><topic>technique des traceurs</topic><topic>tecnicas de trazadores</topic><topic>tracer techniques</topic><topic>via bioquimica del metabolismo</topic><topic>voie biochimique du metabolisme</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van Heerden, Pieter S.</creatorcontrib><creatorcontrib>Towers, G. 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Neil</creatorcontrib><creatorcontrib>Lewis, Norman G.</creatorcontrib><creatorcontrib>University of the Orange Free State, Bloemfontein, South Africa</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>NASA Scientific and Technical Information</collection><collection>NASA Technical Reports Server</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van Heerden, Pieter S.</au><au>Towers, G. H. Neil</au><au>Lewis, Norman G.</au><aucorp>University of the Orange Free State, Bloemfontein, South Africa</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitrogen Metabolism in Lignifying Pinus taeda Cell Cultures (∗)</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1996-05-24</date><risdate>1996</risdate><volume>271</volume><issue>21</issue><spage>12350</spage><epage>12355</epage><pages>12350-12355</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>The primary metabolic fate of phenylalanine, following its deamination in plants, is conscription of its carbon skeleton for lignin, suberin, flavonoid, and related metabolite formation. Since this accounts for ∼30-40% of all organic carbon, an effective means of recycling the liberated ammonium ion must be operative. In order to establish how this occurs, the uptake and metabolism of various 15N-labeled precursors (15N-Phe, 15NH4Cl, 15N-Gln, and 15N-Glu) in lignifying Pinus taeda cell cultures was investigated, using a combination of high performance liquid chromatography, 15N NMR, and gas chromatography-mass spectrometry analyses. It was found that the ammonium ion released during active phenylpropanoid metabolism was not made available for general amino acid/protein synthesis. Rather it was rapidly recycled back to regenerate phenylalanine, thereby providing an effective means of maintaining active phenylpropanoid metabolism with no additional nitrogen requirement. These results strongly suggest that, in lignifying cells, ammonium ion reassimilation is tightly compartmentalized.</abstract><cop>Legacy CDMS</cop><pub>Elsevier Inc</pub><pmid>8647837</pmid><doi>10.1074/jbc.271.21.12350</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; NASA Technical Reports Server; Alma/SFX Local Collection |
| subjects | Amino Acids - metabolism ammonia ammoniac amoniaco biochemical pathways Cells, Cultured Chromatography, High Pressure Liquid fenilalanina Gas Chromatography-Mass Spectrometry Life Sciences (General) lignificacion lignification ligninas lignine lignins Magnetic Resonance Spectroscopy metabolisme de l' azote metabolismo del nitrogeno metabolite metabolites metabolitos Nitrogen - metabolism Nitrogen Isotopes phenylalanine Pinus taeda Space life sciences technique des traceurs tecnicas de trazadores tracer techniques via bioquimica del metabolismo voie biochimique du metabolisme |
| title | Nitrogen Metabolism in Lignifying Pinus taeda Cell Cultures (∗) |
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