Further characterization of Escherichia coli alanyl-tRNA synthetase

Selected physical and thermodynamic parameters for Escherichia coli alanyl-tRNA synthetase (AlaRS) have been determined primarily to assess the quaternary structure of this enzyme. The extinction coefficient (epsilon) at 280 nm was determined experimentally to be 0.71 ml mg-1 cm-1, and the partial s...

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Veröffentlicht in:	Archives of biochemistry and biophysics 1996-04, Vol.328 (2), p.295-301
Hauptverfasser:	Sood, S M, Slattery, C W, Filley, S J, Wu, M X, Hill, K A
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine-tRNA Ligase - chemistry Alanine-tRNA Ligase - isolation & purification Alanine-tRNA Ligase - metabolism Escherichia coli - enzymology Hydrogen-Ion Concentration Isoelectric Focusing Kinetics Lasers Light Macromolecular Substances Molecular Weight Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Scattering, Radiation Thermodynamics Viscosity
Online-Zugang:	Volltext
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