Cloning and biologic activities of a bovine interferon-alpha isolated from the epithelium of a rotavirus-infected calf
A cDNA encoding a distinct bovine (Bo) interferon (IFN) alpha, designated BoIFN-alpha E, was generated from gut epithelial cells isolated from a rotavirus-infected calf. The BoIFN-alpha E cDNA sequence shared a greater than 90% identity with the other BoIFN-alpha subtypes. The cDNA encoding BoIFN-al...
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| Veröffentlicht in: | Journal of interferon & cytokine research 1996-01, Vol.16 (1), p.25-30 |
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| creator | Chaplin, P J Entrican, G Gelder, K I Collins, R A |
| description | A cDNA encoding a distinct bovine (Bo) interferon (IFN) alpha, designated BoIFN-alpha E, was generated from gut epithelial cells isolated from a rotavirus-infected calf. The BoIFN-alpha E cDNA sequence shared a greater than 90% identity with the other BoIFN-alpha subtypes. The cDNA encoding BoIFN-alpha E has been expressed in insect cells using the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) as a vector. Insect cells infected with recombinant virus secreted a protein with a relative molecular mass of 19,500 into the culture medium not observed in cells infected with wild-type AcMNPV. Supernatants harvested from cultures of insect cells infected with the recombinant AcMNPV encoding IFN-alpha E inhibited the replication of Semliki Forest virus in a bovine cell line and typically showed 10(6) dilution units/ml of antiviral activity. However, differences were observed between the activities of recombinant BoIFN-alpha E and BoIFN-alpha 1 1 on the proliferation of WC1+ gamma/delta T cells. Purified ( > 99%) WC1+ gamma/delta T cells failed to proliferate to IFN-alpha 1 1 or concanavalin A and IFN-alpha E acted as a weak proliferative signal to these cells, demonstrating a functional difference between two closely related BoIFN-alpha subtypes. |
| doi_str_mv | 10.1089/jir.1996.16.25 |
| format | Article |
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The BoIFN-alpha E cDNA sequence shared a greater than 90% identity with the other BoIFN-alpha subtypes. The cDNA encoding BoIFN-alpha E has been expressed in insect cells using the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) as a vector. Insect cells infected with recombinant virus secreted a protein with a relative molecular mass of 19,500 into the culture medium not observed in cells infected with wild-type AcMNPV. Supernatants harvested from cultures of insect cells infected with the recombinant AcMNPV encoding IFN-alpha E inhibited the replication of Semliki Forest virus in a bovine cell line and typically showed 10(6) dilution units/ml of antiviral activity. However, differences were observed between the activities of recombinant BoIFN-alpha E and BoIFN-alpha 1 1 on the proliferation of WC1+ gamma/delta T cells. Purified ( > 99%) WC1+ gamma/delta T cells failed to proliferate to IFN-alpha 1 1 or concanavalin A and IFN-alpha E acted as a weak proliferative signal to these cells, demonstrating a functional difference between two closely related BoIFN-alpha subtypes.</description><identifier>ISSN: 1079-9907</identifier><identifier>EISSN: 1557-7465</identifier><identifier>DOI: 10.1089/jir.1996.16.25</identifier><identifier>PMID: 8640447</identifier><language>eng</language><publisher>United States</publisher><subject>Amino Acid Sequence ; Animals ; Antiviral Agents - pharmacology ; Base Sequence ; Cattle ; Cell Division - drug effects ; Cloning, Molecular ; DNA, Complementary - isolation & purification ; Genetic Vectors ; In Vitro Techniques ; Interferon Type I - biosynthesis ; Interferon Type I - genetics ; Interferon Type I - pharmacology ; Interferon-alpha - genetics ; Intestinal Mucosa - metabolism ; Male ; Molecular Sequence Data ; Nucleopolyhedrovirus ; Recombinant Proteins ; rotavirus ; Rotavirus Infections - genetics ; Semliki forest virus - growth & development ; Spodoptera ; Virus Replication - drug effects</subject><ispartof>Journal of interferon & cytokine research, 1996-01, Vol.16 (1), p.25-30</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c321t-795e2c1a7b5a40c102f1f55a0b9c60118a47900889ec69dcc2a599c99b2b290b3</citedby><cites>FETCH-LOGICAL-c321t-795e2c1a7b5a40c102f1f55a0b9c60118a47900889ec69dcc2a599c99b2b290b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3042,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8640447$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaplin, P J</creatorcontrib><creatorcontrib>Entrican, G</creatorcontrib><creatorcontrib>Gelder, K I</creatorcontrib><creatorcontrib>Collins, R A</creatorcontrib><title>Cloning and biologic activities of a bovine interferon-alpha isolated from the epithelium of a rotavirus-infected calf</title><title>Journal of interferon & cytokine research</title><addtitle>J Interferon Cytokine Res</addtitle><description>A cDNA encoding a distinct bovine (Bo) interferon (IFN) alpha, designated BoIFN-alpha E, was generated from gut epithelial cells isolated from a rotavirus-infected calf. The BoIFN-alpha E cDNA sequence shared a greater than 90% identity with the other BoIFN-alpha subtypes. The cDNA encoding BoIFN-alpha E has been expressed in insect cells using the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) as a vector. Insect cells infected with recombinant virus secreted a protein with a relative molecular mass of 19,500 into the culture medium not observed in cells infected with wild-type AcMNPV. Supernatants harvested from cultures of insect cells infected with the recombinant AcMNPV encoding IFN-alpha E inhibited the replication of Semliki Forest virus in a bovine cell line and typically showed 10(6) dilution units/ml of antiviral activity. However, differences were observed between the activities of recombinant BoIFN-alpha E and BoIFN-alpha 1 1 on the proliferation of WC1+ gamma/delta T cells. Purified ( > 99%) WC1+ gamma/delta T cells failed to proliferate to IFN-alpha 1 1 or concanavalin A and IFN-alpha E acted as a weak proliferative signal to these cells, demonstrating a functional difference between two closely related BoIFN-alpha subtypes.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antiviral Agents - pharmacology</subject><subject>Base Sequence</subject><subject>Cattle</subject><subject>Cell Division - drug effects</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary - isolation & purification</subject><subject>Genetic Vectors</subject><subject>In Vitro Techniques</subject><subject>Interferon Type I - biosynthesis</subject><subject>Interferon Type I - genetics</subject><subject>Interferon Type I - pharmacology</subject><subject>Interferon-alpha - genetics</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Nucleopolyhedrovirus</subject><subject>Recombinant Proteins</subject><subject>rotavirus</subject><subject>Rotavirus Infections - genetics</subject><subject>Semliki forest virus - growth & development</subject><subject>Spodoptera</subject><subject>Virus Replication - drug effects</subject><issn>1079-9907</issn><issn>1557-7465</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtPwzAUhS0E4r2yIXliS7h2Yzt3RBUvCYkFZstxbTBy4mInlfj3pGrFynTO8J2zfIRcMagZtHj7FXLNEGXNZM3FATllQqhKNVIczh0UVoigTshZKV8AIFuOx-S4lQ00jTolm2VMQxg-qBlWtAsppo9gqbFj2IQxuEKTp4Z2aRMGR8MwuuxdTkNl4vrT0FBSNKNbUZ9TT8dPR906zBHD1O-WOY1mE_JUqjB4Z7esNdFfkCNvYnGX-zwn7w_3b8un6uX18Xl591LZBWdjpVA4bplRnTANWAbcMy-EgQ6tBMZa0ygEaFt0VuLKWm4EokXseMcRusU5udn9rnP6nlwZdR-KdTGawaWpaNUCx4Vo_wWZAiW5VDNY70CbUynZeb3OoTf5RzPQWyN6NqK3RjSTmot5cL1_nrrerf7wvYLFL4pQiJI</recordid><startdate>199601</startdate><enddate>199601</enddate><creator>Chaplin, P J</creator><creator>Entrican, G</creator><creator>Gelder, K I</creator><creator>Collins, R A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199601</creationdate><title>Cloning and biologic activities of a bovine interferon-alpha isolated from the epithelium of a rotavirus-infected calf</title><author>Chaplin, P J ; Entrican, G ; Gelder, K I ; Collins, R A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-795e2c1a7b5a40c102f1f55a0b9c60118a47900889ec69dcc2a599c99b2b290b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antiviral Agents - pharmacology</topic><topic>Base Sequence</topic><topic>Cattle</topic><topic>Cell Division - drug effects</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary - isolation & purification</topic><topic>Genetic Vectors</topic><topic>In Vitro Techniques</topic><topic>Interferon Type I - biosynthesis</topic><topic>Interferon Type I - genetics</topic><topic>Interferon Type I - pharmacology</topic><topic>Interferon-alpha - genetics</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Nucleopolyhedrovirus</topic><topic>Recombinant Proteins</topic><topic>rotavirus</topic><topic>Rotavirus Infections - genetics</topic><topic>Semliki forest virus - growth & development</topic><topic>Spodoptera</topic><topic>Virus Replication - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaplin, P J</creatorcontrib><creatorcontrib>Entrican, G</creatorcontrib><creatorcontrib>Gelder, K I</creatorcontrib><creatorcontrib>Collins, R A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of interferon & cytokine research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaplin, P J</au><au>Entrican, G</au><au>Gelder, K I</au><au>Collins, R A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and biologic activities of a bovine interferon-alpha isolated from the epithelium of a rotavirus-infected calf</atitle><jtitle>Journal of interferon & cytokine research</jtitle><addtitle>J Interferon Cytokine Res</addtitle><date>1996-01</date><risdate>1996</risdate><volume>16</volume><issue>1</issue><spage>25</spage><epage>30</epage><pages>25-30</pages><issn>1079-9907</issn><eissn>1557-7465</eissn><abstract>A cDNA encoding a distinct bovine (Bo) interferon (IFN) alpha, designated BoIFN-alpha E, was generated from gut epithelial cells isolated from a rotavirus-infected calf. The BoIFN-alpha E cDNA sequence shared a greater than 90% identity with the other BoIFN-alpha subtypes. The cDNA encoding BoIFN-alpha E has been expressed in insect cells using the baculovirus Autographa californica nuclear polyhedrosis virus (AcMNPV) as a vector. Insect cells infected with recombinant virus secreted a protein with a relative molecular mass of 19,500 into the culture medium not observed in cells infected with wild-type AcMNPV. Supernatants harvested from cultures of insect cells infected with the recombinant AcMNPV encoding IFN-alpha E inhibited the replication of Semliki Forest virus in a bovine cell line and typically showed 10(6) dilution units/ml of antiviral activity. However, differences were observed between the activities of recombinant BoIFN-alpha E and BoIFN-alpha 1 1 on the proliferation of WC1+ gamma/delta T cells. Purified ( > 99%) WC1+ gamma/delta T cells failed to proliferate to IFN-alpha 1 1 or concanavalin A and IFN-alpha E acted as a weak proliferative signal to these cells, demonstrating a functional difference between two closely related BoIFN-alpha subtypes.</abstract><cop>United States</cop><pmid>8640447</pmid><doi>10.1089/jir.1996.16.25</doi><tpages>6</tpages></addata></record> |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | Amino Acid Sequence Animals Antiviral Agents - pharmacology Base Sequence Cattle Cell Division - drug effects Cloning, Molecular DNA, Complementary - isolation & purification Genetic Vectors In Vitro Techniques Interferon Type I - biosynthesis Interferon Type I - genetics Interferon Type I - pharmacology Interferon-alpha - genetics Intestinal Mucosa - metabolism Male Molecular Sequence Data Nucleopolyhedrovirus Recombinant Proteins rotavirus Rotavirus Infections - genetics Semliki forest virus - growth & development Spodoptera Virus Replication - drug effects |
| title | Cloning and biologic activities of a bovine interferon-alpha isolated from the epithelium of a rotavirus-infected calf |
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