Clinical efficacy of the amplified Mycobacterium tuberculosis direct test for the diagnosis of pulmonary tuberculosis
The amplified Mycobacterium tuberculosis direct test (MTD) is a rapid diagnostic test based on a nucleic acid amplification technique, which can be used directly on processed clinical specimens. We evaluated the clinical utility of the MTD for diagnosing pulmonary tuberculosis by comparing the sensi...
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| Veröffentlicht in: | American journal of respiratory and critical care medicine 1996-05, Vol.153 (5), p.1606-1610 |
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| container_title | American journal of respiratory and critical care medicine |
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| creator | BRADLEY, S. P REED, S. L CATANZARO, A |
| description | The amplified Mycobacterium tuberculosis direct test (MTD) is a rapid diagnostic test based on a nucleic acid amplification technique, which can be used directly on processed clinical specimens. We evaluated the clinical utility of the MTD for diagnosing pulmonary tuberculosis by comparing the sensitivity and specificity of the test with acid-fast smear, mycobacterial culture, and clinical evaluation. The study included 844 respiratory tract specimens from 421 patients, which were submitted to the microbiology laboratory of our urban teaching hospital over a 6-mo period. Compared with culture, MTD had a sensitivity of 93.6% and specificity of 97.8%. MTD was more sensitive in detecting pulmonary tuberculosis in patients with previously undiagnosed disease (74.7%) than in those with established disease receiving chemotherapy (29.2%), and in smear-positive (95.5%) than in smear-negative (70.0%) disease. There were two false positive MTD results in patients with nontuberculous mycobacteria, for a specificity in this population of 97.3%. We conclude that MTD, when used in conjunction with routine smear and culture, is a useful rapid diagnostic test for suspected pulmonary tuberculosis. |
| doi_str_mv | 10.1164/ajrccm.153.5.8630609 |
| format | Article |
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MTD was more sensitive in detecting pulmonary tuberculosis in patients with previously undiagnosed disease (74.7%) than in those with established disease receiving chemotherapy (29.2%), and in smear-positive (95.5%) than in smear-negative (70.0%) disease. There were two false positive MTD results in patients with nontuberculous mycobacteria, for a specificity in this population of 97.3%. We conclude that MTD, when used in conjunction with routine smear and culture, is a useful rapid diagnostic test for suspected pulmonary tuberculosis.</description><identifier>ISSN: 1073-449X</identifier><identifier>EISSN: 1535-4970</identifier><identifier>DOI: 10.1164/ajrccm.153.5.8630609</identifier><identifier>PMID: 8630609</identifier><language>eng</language><publisher>New York, NY: American Lung Association</publisher><subject>Antitubercular Agents - therapeutic use ; Bacterial diseases ; Bacterial diseases of the respiratory system ; Bacteriological Techniques ; Biological and medical sciences ; DNA Probes ; DNA, Bacterial - analysis ; False Positive Reactions ; Female ; Hospitals, Teaching ; Hospitals, Urban ; Human bacterial diseases ; Humans ; Infectious diseases ; Laboratories, Hospital ; Male ; Medical sciences ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - isolation & purification ; Nontuberculous Mycobacteria - classification ; Nontuberculous Mycobacteria - isolation & purification ; Nucleic Acid Amplification Techniques ; Respiratory System - microbiology ; RNA, Bacterial - analysis ; RNA, Ribosomal - analysis ; Sensitivity and Specificity ; Tuberculosis, Pulmonary - diagnosis ; Tuberculosis, Pulmonary - drug therapy ; Tuberculosis, Pulmonary - microbiology</subject><ispartof>American journal of respiratory and critical care medicine, 1996-05, Vol.153 (5), p.1606-1610</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c294t-ab629e3d575804431220355459721836fb6dba2dd6cd64e0ed2ca09ad77bc1563</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3084411$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8630609$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BRADLEY, S. P</creatorcontrib><creatorcontrib>REED, S. L</creatorcontrib><creatorcontrib>CATANZARO, A</creatorcontrib><title>Clinical efficacy of the amplified Mycobacterium tuberculosis direct test for the diagnosis of pulmonary tuberculosis</title><title>American journal of respiratory and critical care medicine</title><addtitle>Am J Respir Crit Care Med</addtitle><description>The amplified Mycobacterium tuberculosis direct test (MTD) is a rapid diagnostic test based on a nucleic acid amplification technique, which can be used directly on processed clinical specimens. We evaluated the clinical utility of the MTD for diagnosing pulmonary tuberculosis by comparing the sensitivity and specificity of the test with acid-fast smear, mycobacterial culture, and clinical evaluation. The study included 844 respiratory tract specimens from 421 patients, which were submitted to the microbiology laboratory of our urban teaching hospital over a 6-mo period. Compared with culture, MTD had a sensitivity of 93.6% and specificity of 97.8%. MTD was more sensitive in detecting pulmonary tuberculosis in patients with previously undiagnosed disease (74.7%) than in those with established disease receiving chemotherapy (29.2%), and in smear-positive (95.5%) than in smear-negative (70.0%) disease. There were two false positive MTD results in patients with nontuberculous mycobacteria, for a specificity in this population of 97.3%. We conclude that MTD, when used in conjunction with routine smear and culture, is a useful rapid diagnostic test for suspected pulmonary tuberculosis.</description><subject>Antitubercular Agents - therapeutic use</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the respiratory system</subject><subject>Bacteriological Techniques</subject><subject>Biological and medical sciences</subject><subject>DNA Probes</subject><subject>DNA, Bacterial - analysis</subject><subject>False Positive Reactions</subject><subject>Female</subject><subject>Hospitals, Teaching</subject><subject>Hospitals, Urban</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Laboratories, Hospital</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - isolation & purification</subject><subject>Nontuberculous Mycobacteria - classification</subject><subject>Nontuberculous Mycobacteria - isolation & purification</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Respiratory System - microbiology</subject><subject>RNA, Bacterial - analysis</subject><subject>RNA, Ribosomal - analysis</subject><subject>Sensitivity and Specificity</subject><subject>Tuberculosis, Pulmonary - diagnosis</subject><subject>Tuberculosis, Pulmonary - drug therapy</subject><subject>Tuberculosis, Pulmonary - microbiology</subject><issn>1073-449X</issn><issn>1535-4970</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUclOwzAQtRCoQOEPQPIBcUvxnuSIKjapiAtI3CLHC7jKhh0f-veYNirixGlm9Jax5wFwgdECY8Fu5Nor1S4wpwu-KARFApUH4CTNPGNljg5Tj3KaMVa-H4PTENYIYVJgNAOziX4C4rJxnVOygcbaVNUG9haOnwbKdmicdUbD543qa6lG411s4Rhr41Vs-uAC1M4bNcLRhBHa3m-F2smPbosmpyE2bd9Jv_mjOwNHVjbBnE91Dt7u716Xj9nq5eFpebvKFCnZmMlakNJQzXNeIMYoJgRRzhkvc4ILKmwtdC2J1kJpwQwymiiJSqnzvFaYCzoH1zvfwfdfMT2yal1QpmlkZ_oYqrxA6SAY_UtMZoSItHMO2I6ofB-CN7YavGvT_yqMqp9Yql0sSUErXk13TrLLyT_WrdF70S9-NeEypDCsl51yYU-jqGAMY_oNtxaZDA</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>BRADLEY, S. 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L</au><au>CATANZARO, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical efficacy of the amplified Mycobacterium tuberculosis direct test for the diagnosis of pulmonary tuberculosis</atitle><jtitle>American journal of respiratory and critical care medicine</jtitle><addtitle>Am J Respir Crit Care Med</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>153</volume><issue>5</issue><spage>1606</spage><epage>1610</epage><pages>1606-1610</pages><issn>1073-449X</issn><eissn>1535-4970</eissn><abstract>The amplified Mycobacterium tuberculosis direct test (MTD) is a rapid diagnostic test based on a nucleic acid amplification technique, which can be used directly on processed clinical specimens. We evaluated the clinical utility of the MTD for diagnosing pulmonary tuberculosis by comparing the sensitivity and specificity of the test with acid-fast smear, mycobacterial culture, and clinical evaluation. The study included 844 respiratory tract specimens from 421 patients, which were submitted to the microbiology laboratory of our urban teaching hospital over a 6-mo period. Compared with culture, MTD had a sensitivity of 93.6% and specificity of 97.8%. MTD was more sensitive in detecting pulmonary tuberculosis in patients with previously undiagnosed disease (74.7%) than in those with established disease receiving chemotherapy (29.2%), and in smear-positive (95.5%) than in smear-negative (70.0%) disease. There were two false positive MTD results in patients with nontuberculous mycobacteria, for a specificity in this population of 97.3%. We conclude that MTD, when used in conjunction with routine smear and culture, is a useful rapid diagnostic test for suspected pulmonary tuberculosis.</abstract><cop>New York, NY</cop><pub>American Lung Association</pub><pmid>8630609</pmid><doi>10.1164/ajrccm.153.5.8630609</doi><tpages>5</tpages></addata></record> |
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| subjects | Antitubercular Agents - therapeutic use Bacterial diseases Bacterial diseases of the respiratory system Bacteriological Techniques Biological and medical sciences DNA Probes DNA, Bacterial - analysis False Positive Reactions Female Hospitals, Teaching Hospitals, Urban Human bacterial diseases Humans Infectious diseases Laboratories, Hospital Male Medical sciences Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Nontuberculous Mycobacteria - classification Nontuberculous Mycobacteria - isolation & purification Nucleic Acid Amplification Techniques Respiratory System - microbiology RNA, Bacterial - analysis RNA, Ribosomal - analysis Sensitivity and Specificity Tuberculosis, Pulmonary - diagnosis Tuberculosis, Pulmonary - drug therapy Tuberculosis, Pulmonary - microbiology |
| title | Clinical efficacy of the amplified Mycobacterium tuberculosis direct test for the diagnosis of pulmonary tuberculosis |
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