Within Germinal Centers, Isotype Switching of Immunoglobulin Genes Occurs after the Onset of Somatic Mutation

Human tonsillar B cells were separated into naive IgD +CD38 −CD23 − (Bm1) and IgD +CD38 −CD23 + (Bm2), germinal center IgD −CD38 +CD23 − centroblasts (Bm3) and IgD −CD38 +CD77 − centrocytes (Bm4), and memory IgD −CD38 − (Bm5) subsets. Previous IgV H sequence analysis concluded that the triggering of...

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Veröffentlicht in:Immunity (Cambridge, Mass.) Mass.), 1996-03, Vol.4 (3), p.241-250
Hauptverfasser: Liu, Yong Jun, Malisan, Florence, de Bouteiller, Odette, Guret, Christiane, Lebecque, Serge, Banchereau, Jacques, Mills, Frederick C, Max, Edward E, Martinez-Valdez, Héctor
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container_end_page 250
container_issue 3
container_start_page 241
container_title Immunity (Cambridge, Mass.)
container_volume 4
creator Liu, Yong Jun
Malisan, Florence
de Bouteiller, Odette
Guret, Christiane
Lebecque, Serge
Banchereau, Jacques
Mills, Frederick C
Max, Edward E
Martinez-Valdez, Héctor
description Human tonsillar B cells were separated into naive IgD +CD38 −CD23 − (Bm1) and IgD +CD38 −CD23 + (Bm2), germinal center IgD −CD38 +CD23 − centroblasts (Bm3) and IgD −CD38 +CD77 − centrocytes (Bm4), and memory IgD −CD38 − (Bm5) subsets. Previous IgV H sequence analysis concluded that the triggering of somatic mutations occurs during the transition from Bm2 subset into the Bm3 subset. To determine the initiation of isotype switching, sterile transcript expression was analyzed by amplification, cloning, and sequencing. A selective sterile Iγ, Iα, and Iε expression was observed at centrocyte (Bm4) stage, suggesting that isotype switch is triggered within germinal centers, after somatic mutation is initiated within centroblasts (Bm3). Finally, the high level of 5′Sγ–Sμ3′ DNA switching circles observed in germinal center B cells indicates that within human tonsils, germinal center is a major location for isotype switching.
doi_str_mv 10.1016/S1074-7613(00)80432-X
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ispartof Immunity (Cambridge, Mass.), 1996-03, Vol.4 (3), p.241-250
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source MEDLINE; Cell Press Free Archives; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals
subjects B-Lymphocytes - immunology
Base Sequence
Cell Line
DNA - analysis
DNA Damage - immunology
Gene Rearrangement, B-Lymphocyte - immunology
Genes, Immunoglobulin
Germinal Center - chemistry
Germinal Center - immunology
Germinal Center - metabolism
Histocompatibility Antigens Class II - genetics
Humans
Immunoglobulin Class Switching - immunology
Molecular Sequence Data
Mutation - immunology
Palatine Tonsil - immunology
Sequence Deletion - immunology
Transcription, Genetic - immunology
title Within Germinal Centers, Isotype Switching of Immunoglobulin Genes Occurs after the Onset of Somatic Mutation
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