Barley beta-D-glucan exohydrolases with beta-D-glucosidase activity: purification, characterization, and determination of primary structure from a cDNA clone

Two beta-glucan exohydrolases of apparent molecular masses 69,000 and 71,000 Da have been purified from extracts of 8-day germinated barley grains and are designated isoenzymes ExoI and ExoII, respectively. The sequences of their first 52 NH2-terminal amino acids show 64% positional identity. Both e...

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