Enzyme Labeling of Steroids by the N-Succinimidyl Ester Method. Preparation of Glucose Oxidase-Labeled Antigen for Use in Enzyme Immunoassay

Enzyme labeling of a steroid with glucose oxidase by the N-succinimidyl ester method was investigated. The activated ester of a testosterone derivative was treated with glucose oxidase to give a labeled antigen. Various molar ratios of steroid to enzyme, ranging from 2 to 100, were employed; the deg...

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