The developmental expression of cysteine proteinases in Schistosoma mansoni
In this study several probes were used to identify and characterize cysteine proteinases (CP) from Schistosoma mansoni developmental stages. Proteinase activity was detected in all developmental stages using fluorogenic substrates. Specific activity was 4- to 11-fold higher against CBZ-phe-arg-AFC t...
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| Veröffentlicht in: | International journal for parasitology 1996, Vol.26 (1), p.109-112 |
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| container_title | International journal for parasitology |
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| creator | Monroy, Fernando P. Dresden, Marc H. |
| description | In this study several probes were used to identify and characterize cysteine proteinases (CP) from
Schistosoma mansoni developmental stages. Proteinase activity was detected in all developmental stages using fluorogenic substrates. Specific activity was 4- to 11-fold higher against CBZ-phe-arg-AFC than against CBZ-arg-arg-AFC. A 32 kDa
S. mansoni CP (Sm32) was identified in all stages by the radiolabelled active site CP inhibitor, CBZ-[
125I]tyr-ala-CHN
2. A second CP, of 31 kDa (Sm31) was only detected in adult worms, primarily female worms and late schistosomula. Monoclonal antibodies for Sm32 and Sm31 reacted with homologous CP only in adult worms and late schistosomula. This study defines the developmental expression of CP activity in
S. mansoni. |
| doi_str_mv | 10.1016/0020-7519(95)00077-1 |
| format | Article |
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Schistosoma mansoni developmental stages. Proteinase activity was detected in all developmental stages using fluorogenic substrates. Specific activity was 4- to 11-fold higher against CBZ-phe-arg-AFC than against CBZ-arg-arg-AFC. A 32 kDa
S. mansoni CP (Sm32) was identified in all stages by the radiolabelled active site CP inhibitor, CBZ-[
125I]tyr-ala-CHN
2. A second CP, of 31 kDa (Sm31) was only detected in adult worms, primarily female worms and late schistosomula. Monoclonal antibodies for Sm32 and Sm31 reacted with homologous CP only in adult worms and late schistosomula. This study defines the developmental expression of CP activity in
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Schistosoma mansoni developmental stages. Proteinase activity was detected in all developmental stages using fluorogenic substrates. Specific activity was 4- to 11-fold higher against CBZ-phe-arg-AFC than against CBZ-arg-arg-AFC. A 32 kDa
S. mansoni CP (Sm32) was identified in all stages by the radiolabelled active site CP inhibitor, CBZ-[
125I]tyr-ala-CHN
2. A second CP, of 31 kDa (Sm31) was only detected in adult worms, primarily female worms and late schistosomula. Monoclonal antibodies for Sm32 and Sm31 reacted with homologous CP only in adult worms and late schistosomula. This study defines the developmental expression of CP activity in
S. mansoni.</description><subject>Animals</subject><subject>Biochemistry. Physiology. Immunology. Molecular biology</subject><subject>Biological and medical sciences</subject><subject>Biomphalaria</subject><subject>cathepsins</subject><subject>Cysteine Endopeptidases - analysis</subject><subject>cysteine proteinases</subject><subject>enzyme inhibitors</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Invertebrates</subject><subject>Male</subject><subject>Mice</subject><subject>Nemathelminthia. Plathelmintha</subject><subject>Physiology. Development</subject><subject>proteases</subject><subject>proteinases</subject><subject>S mansoni</subject><subject>Schistosoma mansoni - enzymology</subject><subject>Schistosoma mansoni - growth & development</subject><subject>Schistosomes</subject><subject>Sex Characteristics</subject><subject>Substrate Specificity</subject><subject>Trematode</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVpSTeb_oMWfCghOTidsS1bvhRKyEdpIIcmZyFLY6JiS1uNNzT_PnZ32WNOGjHPvMw8QnxGuEDA-htAAXkjsT1r5TkANE2O78QKVdPmgKV8L1YH5KM4Zv4DgLKsqiNx1GKrpJIr8evhiTJHzzTEzUhhMkNG_zaJmH0MWewz-8IT-UDZJsWlMEyc-ZD9tk-ep8hxNNloAsfgT8SH3gxMn_bvWjxeXz1c3uZ39zc_L3_c5bYs5ZSXyijqZNkUsqqRUHYW66Kre3JUgLPgjJw_2HSqL0xbu7ozNbia-gIUSizX4nSXO6_0d0s86dGzpWEwgeKWdaNmPTifuhbVDrQpMifq9Sb50aQXjaAXh3oRpBdBupX6v0O95H_Z52-7kdxhaC9t7n_d9w1bM_TJBOv5gBWtqkCqGfu-w2h28ewpabaegiXnE9lJu-jf3uMVgmCN-Q</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Monroy, Fernando P.</creator><creator>Dresden, Marc H.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>The developmental expression of cysteine proteinases in Schistosoma mansoni</title><author>Monroy, Fernando P. ; Dresden, Marc H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-38a8eb53725461e15bc162b6fede20dc0da56fe17b8f2a96d6ba60d6ef2081513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biochemistry. Physiology. Immunology. Molecular biology</topic><topic>Biological and medical sciences</topic><topic>Biomphalaria</topic><topic>cathepsins</topic><topic>Cysteine Endopeptidases - analysis</topic><topic>cysteine proteinases</topic><topic>enzyme inhibitors</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Invertebrates</topic><topic>Male</topic><topic>Mice</topic><topic>Nemathelminthia. Plathelmintha</topic><topic>Physiology. Development</topic><topic>proteases</topic><topic>proteinases</topic><topic>S mansoni</topic><topic>Schistosoma mansoni - enzymology</topic><topic>Schistosoma mansoni - growth & development</topic><topic>Schistosomes</topic><topic>Sex Characteristics</topic><topic>Substrate Specificity</topic><topic>Trematode</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Monroy, Fernando P.</creatorcontrib><creatorcontrib>Dresden, Marc H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Monroy, Fernando P.</au><au>Dresden, Marc H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The developmental expression of cysteine proteinases in Schistosoma mansoni</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>1996</date><risdate>1996</risdate><volume>26</volume><issue>1</issue><spage>109</spage><epage>112</epage><pages>109-112</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>In this study several probes were used to identify and characterize cysteine proteinases (CP) from
Schistosoma mansoni developmental stages. Proteinase activity was detected in all developmental stages using fluorogenic substrates. Specific activity was 4- to 11-fold higher against CBZ-phe-arg-AFC than against CBZ-arg-arg-AFC. A 32 kDa
S. mansoni CP (Sm32) was identified in all stages by the radiolabelled active site CP inhibitor, CBZ-[
125I]tyr-ala-CHN
2. A second CP, of 31 kDa (Sm31) was only detected in adult worms, primarily female worms and late schistosomula. Monoclonal antibodies for Sm32 and Sm31 reacted with homologous CP only in adult worms and late schistosomula. This study defines the developmental expression of CP activity in
S. mansoni.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>9198585</pmid><doi>10.1016/0020-7519(95)00077-1</doi><tpages>4</tpages></addata></record> |
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| ispartof | International journal for parasitology, 1996, Vol.26 (1), p.109-112 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Biomphalaria cathepsins Cysteine Endopeptidases - analysis cysteine proteinases enzyme inhibitors Female Fundamental and applied biological sciences. Psychology Invertebrates Male Mice Nemathelminthia. Plathelmintha Physiology. Development proteases proteinases S mansoni Schistosoma mansoni - enzymology Schistosoma mansoni - growth & development Schistosomes Sex Characteristics Substrate Specificity Trematode |
| title | The developmental expression of cysteine proteinases in Schistosoma mansoni |
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