Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype
Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte vi...
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Veröffentlicht in: | The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 1996-03, Vol.51A (2), p.B132-B140 |
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creator | Lohmiller, Jeffrey J. Roellich, Kathleen M. Toledano, Alicia Rabinovitch, Peter S. Wolf, Norman S. Grossmann, Angelika |
description | Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte viability, proliferation, GSH content, and calcium signaling were measured. Before exposure, proliferation of T-lymphocytes from young mice was greater than that of old; following exposure, the converse was true. This was in spite of the fact that old mice had lower total GSH levels and greater levels of glutathione disulfide. After oxidative challenge, intracellular calcium responses to anti-CD3 were decreased in naive T-lymphocytes from all mice, while memory lymphocytes were less affected. Higher proportions of memory lymphocytes in old mice resulted in their greater overall preservation of lymphocyte function following oxidative injury, contrary to expectations that lower lymphocyte GSH content with age would increase susceptibility to oxidative stress. |
doi_str_mv | 10.1093/gerona/51A.2.B132 |
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Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte viability, proliferation, GSH content, and calcium signaling were measured. Before exposure, proliferation of T-lymphocytes from young mice was greater than that of old; following exposure, the converse was true. This was in spite of the fact that old mice had lower total GSH levels and greater levels of glutathione disulfide. After oxidative challenge, intracellular calcium responses to anti-CD3 were decreased in naive T-lymphocytes from all mice, while memory lymphocytes were less affected. Higher proportions of memory lymphocytes in old mice resulted in their greater overall preservation of lymphocyte function following oxidative injury, contrary to expectations that lower lymphocyte GSH content with age would increase susceptibility to oxidative stress.</description><identifier>ISSN: 1079-5006</identifier><identifier>EISSN: 1758-535X</identifier><identifier>DOI: 10.1093/gerona/51A.2.B132</identifier><identifier>PMID: 8612097</identifier><language>eng</language><publisher>United States: The Gerontological Society of America</publisher><subject>Aging ; Aging - physiology ; Animals ; Cell Survival ; Cellular biology ; Flow Cytometry ; Glutathione - analysis ; Immunity (Disease) ; Immunologic Memory ; Male ; Mice ; Mice, Inbred Strains ; Oxidative Stress - physiology ; Phenotype ; Rodents ; T-Lymphocytes - chemistry ; T-Lymphocytes - physiology</subject><ispartof>The journals of gerontology. 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Higher proportions of memory lymphocytes in old mice resulted in their greater overall preservation of lymphocyte function following oxidative injury, contrary to expectations that lower lymphocyte GSH content with age would increase susceptibility to oxidative stress.</description><subject>Aging</subject><subject>Aging - physiology</subject><subject>Animals</subject><subject>Cell Survival</subject><subject>Cellular biology</subject><subject>Flow Cytometry</subject><subject>Glutathione - analysis</subject><subject>Immunity (Disease)</subject><subject>Immunologic Memory</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Oxidative Stress - physiology</subject><subject>Phenotype</subject><subject>Rodents</subject><subject>T-Lymphocytes - chemistry</subject><subject>T-Lymphocytes - physiology</subject><issn>1079-5006</issn><issn>1758-535X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>K30</sourceid><recordid>eNp1kc1u1DAUhS0EKqXwACyQLJDYZeqf-G85TIEWddoRLVLFxnKSm5mUSTzYCWoegPeupzPqolK98LXO-e7VlQ9C7ymZUGL48RKC79yxoNMJm3yhnL1Ah1QJnQkubl6mN1EmE4TI1-hNjLdkewQ7QAdaUkaMOkT_p0uo8HwITQf4OluP7Wbly7GHiKcB8Nyn6yfEJvau63Hv8eVdU7m--Qf4xLVumcoAW71fAV4EqHzbdK4rAfv6QZvBeh3xwscIMTbd8kGcQ-vDiBcr6Hw_buAtelW7dYR3-3qEfn37ej07zc4vv5_NpudZmXPWZ4IyWeSGqzI3xpEir2qjQGtdCyYqwwqqFM-FyWntpDZEM8EqqktVOM2oY_wIfd7N3QT_d4DY27aJZdrQdeCHaJUmVBJGE_jxCXjrh9Cl3SwjWrJcaZGgT89BVCtFuaBaJ4ruqDKkTwhQ201oWhdGS4ndpmh3KdqUomV2m2Lq-bCfPBQtVI8d-9iSn-38lAvcPdou_LFScSXs6c1vqy8uruTVjxMr-T0Pqaef</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Lohmiller, Jeffrey J.</creator><creator>Roellich, Kathleen M.</creator><creator>Toledano, Alicia</creator><creator>Rabinovitch, Peter S.</creator><creator>Wolf, Norman S.</creator><creator>Grossmann, Angelika</creator><general>The Gerontological Society of America</general><general>Gerontological Society of America</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOIBA</scope><scope>K30</scope><scope>PAAUG</scope><scope>PAWHS</scope><scope>PAWZZ</scope><scope>PAXOH</scope><scope>PBHAV</scope><scope>PBQSW</scope><scope>PBYQZ</scope><scope>PCIWU</scope><scope>PCMID</scope><scope>PCZJX</scope><scope>PDGRG</scope><scope>PDWWI</scope><scope>PETMR</scope><scope>PFVGT</scope><scope>PGXDX</scope><scope>PIHIL</scope><scope>PISVA</scope><scope>PJCTQ</scope><scope>PJTMS</scope><scope>PLCHJ</scope><scope>PMHAD</scope><scope>PNQDJ</scope><scope>POUND</scope><scope>PPLAD</scope><scope>PQAPC</scope><scope>PQCAN</scope><scope>PQCMW</scope><scope>PQEME</scope><scope>PQHKH</scope><scope>PQMID</scope><scope>PQNCT</scope><scope>PQNET</scope><scope>PQSCT</scope><scope>PQSET</scope><scope>PSVJG</scope><scope>PVMQY</scope><scope>PZGFC</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>19960301</creationdate><title>Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype</title><author>Lohmiller, Jeffrey J. ; 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Series A, Biological sciences and medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lohmiller, Jeffrey J.</au><au>Roellich, Kathleen M.</au><au>Toledano, Alicia</au><au>Rabinovitch, Peter S.</au><au>Wolf, Norman S.</au><au>Grossmann, Angelika</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype</atitle><jtitle>The journals of gerontology. Series A, Biological sciences and medical sciences</jtitle><addtitle>J Gerontol A Biol Sci Med Sci</addtitle><date>1996-03-01</date><risdate>1996</risdate><volume>51A</volume><issue>2</issue><spage>B132</spage><epage>B140</epage><pages>B132-B140</pages><issn>1079-5006</issn><eissn>1758-535X</eissn><abstract>Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte viability, proliferation, GSH content, and calcium signaling were measured. Before exposure, proliferation of T-lymphocytes from young mice was greater than that of old; following exposure, the converse was true. This was in spite of the fact that old mice had lower total GSH levels and greater levels of glutathione disulfide. After oxidative challenge, intracellular calcium responses to anti-CD3 were decreased in naive T-lymphocytes from all mice, while memory lymphocytes were less affected. Higher proportions of memory lymphocytes in old mice resulted in their greater overall preservation of lymphocyte function following oxidative injury, contrary to expectations that lower lymphocyte GSH content with age would increase susceptibility to oxidative stress.</abstract><cop>United States</cop><pub>The Gerontological Society of America</pub><pmid>8612097</pmid><doi>10.1093/gerona/51A.2.B132</doi><oa>free_for_read</oa></addata></record> |
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source | MEDLINE; Periodicals Index Online; Oxford University Press Journals All Titles (1996-Current) |
subjects | Aging Aging - physiology Animals Cell Survival Cellular biology Flow Cytometry Glutathione - analysis Immunity (Disease) Immunologic Memory Male Mice Mice, Inbred Strains Oxidative Stress - physiology Phenotype Rodents T-Lymphocytes - chemistry T-Lymphocytes - physiology |
title | Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype |
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