Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype

Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte vi...

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Veröffentlicht in:The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 1996-03, Vol.51A (2), p.B132-B140
Hauptverfasser: Lohmiller, Jeffrey J., Roellich, Kathleen M., Toledano, Alicia, Rabinovitch, Peter S., Wolf, Norman S., Grossmann, Angelika
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container_end_page B140
container_issue 2
container_start_page B132
container_title The journals of gerontology. Series A, Biological sciences and medical sciences
container_volume 51A
creator Lohmiller, Jeffrey J.
Roellich, Kathleen M.
Toledano, Alicia
Rabinovitch, Peter S.
Wolf, Norman S.
Grossmann, Angelika
description Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte viability, proliferation, GSH content, and calcium signaling were measured. Before exposure, proliferation of T-lymphocytes from young mice was greater than that of old; following exposure, the converse was true. This was in spite of the fact that old mice had lower total GSH levels and greater levels of glutathione disulfide. After oxidative challenge, intracellular calcium responses to anti-CD3 were decreased in naive T-lymphocytes from all mice, while memory lymphocytes were less affected. Higher proportions of memory lymphocytes in old mice resulted in their greater overall preservation of lymphocyte function following oxidative injury, contrary to expectations that lower lymphocyte GSH content with age would increase susceptibility to oxidative stress.
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Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte viability, proliferation, GSH content, and calcium signaling were measured. Before exposure, proliferation of T-lymphocytes from young mice was greater than that of old; following exposure, the converse was true. This was in spite of the fact that old mice had lower total GSH levels and greater levels of glutathione disulfide. After oxidative challenge, intracellular calcium responses to anti-CD3 were decreased in naive T-lymphocytes from all mice, while memory lymphocytes were less affected. 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identifier ISSN: 1079-5006
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source MEDLINE; Periodicals Index Online; Oxford University Press Journals All Titles (1996-Current)
subjects Aging
Aging - physiology
Animals
Cell Survival
Cellular biology
Flow Cytometry
Glutathione - analysis
Immunity (Disease)
Immunologic Memory
Male
Mice
Mice, Inbred Strains
Oxidative Stress - physiology
Phenotype
Rodents
T-Lymphocytes - chemistry
T-Lymphocytes - physiology
title Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype
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