Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype
Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte vi...
Gespeichert in:
Veröffentlicht in: | The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 1996-03, Vol.51A (2), p.B132-B140 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | B140 |
---|---|
container_issue | 2 |
container_start_page | B132 |
container_title | The journals of gerontology. Series A, Biological sciences and medical sciences |
container_volume | 51A |
creator | Lohmiller, Jeffrey J. Roellich, Kathleen M. Toledano, Alicia Rabinovitch, Peter S. Wolf, Norman S. Grossmann, Angelika |
description | Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte viability, proliferation, GSH content, and calcium signaling were measured. Before exposure, proliferation of T-lymphocytes from young mice was greater than that of old; following exposure, the converse was true. This was in spite of the fact that old mice had lower total GSH levels and greater levels of glutathione disulfide. After oxidative challenge, intracellular calcium responses to anti-CD3 were decreased in naive T-lymphocytes from all mice, while memory lymphocytes were less affected. Higher proportions of memory lymphocytes in old mice resulted in their greater overall preservation of lymphocyte function following oxidative injury, contrary to expectations that lower lymphocyte GSH content with age would increase susceptibility to oxidative stress. |
doi_str_mv | 10.1093/gerona/51A.2.B132 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78016021</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1877135188</sourcerecordid><originalsourceid>FETCH-LOGICAL-c432t-5126b4937c499a0b4df97e888f525d92b177345941fa68908252d18c7ba821a23</originalsourceid><addsrcrecordid>eNp1kc1u1DAUhS0EKqXwACyQLJDYZeqf-G85TIEWddoRLVLFxnKSm5mUSTzYCWoegPeupzPqolK98LXO-e7VlQ9C7ymZUGL48RKC79yxoNMJm3yhnL1Ah1QJnQkubl6mN1EmE4TI1-hNjLdkewQ7QAdaUkaMOkT_p0uo8HwITQf4OluP7Wbly7GHiKcB8Nyn6yfEJvau63Hv8eVdU7m--Qf4xLVumcoAW71fAV4EqHzbdK4rAfv6QZvBeh3xwscIMTbd8kGcQ-vDiBcr6Hw_buAtelW7dYR3-3qEfn37ej07zc4vv5_NpudZmXPWZ4IyWeSGqzI3xpEir2qjQGtdCyYqwwqqFM-FyWntpDZEM8EqqktVOM2oY_wIfd7N3QT_d4DY27aJZdrQdeCHaJUmVBJGE_jxCXjrh9Cl3SwjWrJcaZGgT89BVCtFuaBaJ4ruqDKkTwhQ201oWhdGS4ndpmh3KdqUomV2m2Lq-bCfPBQtVI8d-9iSn-38lAvcPdou_LFScSXs6c1vqy8uruTVjxMr-T0Pqaef</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1877135188</pqid></control><display><type>article</type><title>Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype</title><source>MEDLINE</source><source>Periodicals Index Online</source><source>Oxford University Press Journals</source><creator>Lohmiller, Jeffrey J. ; Roellich, Kathleen M. ; Toledano, Alicia ; Rabinovitch, Peter S. ; Wolf, Norman S. ; Grossmann, Angelika</creator><creatorcontrib>Lohmiller, Jeffrey J. ; Roellich, Kathleen M. ; Toledano, Alicia ; Rabinovitch, Peter S. ; Wolf, Norman S. ; Grossmann, Angelika</creatorcontrib><description>Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte viability, proliferation, GSH content, and calcium signaling were measured. Before exposure, proliferation of T-lymphocytes from young mice was greater than that of old; following exposure, the converse was true. This was in spite of the fact that old mice had lower total GSH levels and greater levels of glutathione disulfide. After oxidative challenge, intracellular calcium responses to anti-CD3 were decreased in naive T-lymphocytes from all mice, while memory lymphocytes were less affected. Higher proportions of memory lymphocytes in old mice resulted in their greater overall preservation of lymphocyte function following oxidative injury, contrary to expectations that lower lymphocyte GSH content with age would increase susceptibility to oxidative stress.</description><identifier>ISSN: 1079-5006</identifier><identifier>EISSN: 1758-535X</identifier><identifier>DOI: 10.1093/gerona/51A.2.B132</identifier><identifier>PMID: 8612097</identifier><language>eng</language><publisher>United States: The Gerontological Society of America</publisher><subject>Aging ; Aging - physiology ; Animals ; Cell Survival ; Cellular biology ; Flow Cytometry ; Glutathione - analysis ; Immunity (Disease) ; Immunologic Memory ; Male ; Mice ; Mice, Inbred Strains ; Oxidative Stress - physiology ; Phenotype ; Rodents ; T-Lymphocytes - chemistry ; T-Lymphocytes - physiology</subject><ispartof>The journals of gerontology. Series A, Biological sciences and medical sciences, 1996-03, Vol.51A (2), p.B132-B140</ispartof><rights>Copyright Gerontological Society of America, Incorporated Mar 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-5126b4937c499a0b4df97e888f525d92b177345941fa68908252d18c7ba821a23</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27876,27931,27932</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8612097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lohmiller, Jeffrey J.</creatorcontrib><creatorcontrib>Roellich, Kathleen M.</creatorcontrib><creatorcontrib>Toledano, Alicia</creatorcontrib><creatorcontrib>Rabinovitch, Peter S.</creatorcontrib><creatorcontrib>Wolf, Norman S.</creatorcontrib><creatorcontrib>Grossmann, Angelika</creatorcontrib><title>Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype</title><title>The journals of gerontology. Series A, Biological sciences and medical sciences</title><addtitle>J Gerontol A Biol Sci Med Sci</addtitle><description>Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte viability, proliferation, GSH content, and calcium signaling were measured. Before exposure, proliferation of T-lymphocytes from young mice was greater than that of old; following exposure, the converse was true. This was in spite of the fact that old mice had lower total GSH levels and greater levels of glutathione disulfide. After oxidative challenge, intracellular calcium responses to anti-CD3 were decreased in naive T-lymphocytes from all mice, while memory lymphocytes were less affected. Higher proportions of memory lymphocytes in old mice resulted in their greater overall preservation of lymphocyte function following oxidative injury, contrary to expectations that lower lymphocyte GSH content with age would increase susceptibility to oxidative stress.</description><subject>Aging</subject><subject>Aging - physiology</subject><subject>Animals</subject><subject>Cell Survival</subject><subject>Cellular biology</subject><subject>Flow Cytometry</subject><subject>Glutathione - analysis</subject><subject>Immunity (Disease)</subject><subject>Immunologic Memory</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Oxidative Stress - physiology</subject><subject>Phenotype</subject><subject>Rodents</subject><subject>T-Lymphocytes - chemistry</subject><subject>T-Lymphocytes - physiology</subject><issn>1079-5006</issn><issn>1758-535X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>K30</sourceid><recordid>eNp1kc1u1DAUhS0EKqXwACyQLJDYZeqf-G85TIEWddoRLVLFxnKSm5mUSTzYCWoegPeupzPqolK98LXO-e7VlQ9C7ymZUGL48RKC79yxoNMJm3yhnL1Ah1QJnQkubl6mN1EmE4TI1-hNjLdkewQ7QAdaUkaMOkT_p0uo8HwITQf4OluP7Wbly7GHiKcB8Nyn6yfEJvau63Hv8eVdU7m--Qf4xLVumcoAW71fAV4EqHzbdK4rAfv6QZvBeh3xwscIMTbd8kGcQ-vDiBcr6Hw_buAtelW7dYR3-3qEfn37ej07zc4vv5_NpudZmXPWZ4IyWeSGqzI3xpEir2qjQGtdCyYqwwqqFM-FyWntpDZEM8EqqktVOM2oY_wIfd7N3QT_d4DY27aJZdrQdeCHaJUmVBJGE_jxCXjrh9Cl3SwjWrJcaZGgT89BVCtFuaBaJ4ruqDKkTwhQ201oWhdGS4ndpmh3KdqUomV2m2Lq-bCfPBQtVI8d-9iSn-38lAvcPdou_LFScSXs6c1vqy8uruTVjxMr-T0Pqaef</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Lohmiller, Jeffrey J.</creator><creator>Roellich, Kathleen M.</creator><creator>Toledano, Alicia</creator><creator>Rabinovitch, Peter S.</creator><creator>Wolf, Norman S.</creator><creator>Grossmann, Angelika</creator><general>The Gerontological Society of America</general><general>Gerontological Society of America</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOIBA</scope><scope>K30</scope><scope>PAAUG</scope><scope>PAWHS</scope><scope>PAWZZ</scope><scope>PAXOH</scope><scope>PBHAV</scope><scope>PBQSW</scope><scope>PBYQZ</scope><scope>PCIWU</scope><scope>PCMID</scope><scope>PCZJX</scope><scope>PDGRG</scope><scope>PDWWI</scope><scope>PETMR</scope><scope>PFVGT</scope><scope>PGXDX</scope><scope>PIHIL</scope><scope>PISVA</scope><scope>PJCTQ</scope><scope>PJTMS</scope><scope>PLCHJ</scope><scope>PMHAD</scope><scope>PNQDJ</scope><scope>POUND</scope><scope>PPLAD</scope><scope>PQAPC</scope><scope>PQCAN</scope><scope>PQCMW</scope><scope>PQEME</scope><scope>PQHKH</scope><scope>PQMID</scope><scope>PQNCT</scope><scope>PQNET</scope><scope>PQSCT</scope><scope>PQSET</scope><scope>PSVJG</scope><scope>PVMQY</scope><scope>PZGFC</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>19960301</creationdate><title>Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype</title><author>Lohmiller, Jeffrey J. ; Roellich, Kathleen M. ; Toledano, Alicia ; Rabinovitch, Peter S. ; Wolf, Norman S. ; Grossmann, Angelika</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-5126b4937c499a0b4df97e888f525d92b177345941fa68908252d18c7ba821a23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Aging</topic><topic>Aging - physiology</topic><topic>Animals</topic><topic>Cell Survival</topic><topic>Cellular biology</topic><topic>Flow Cytometry</topic><topic>Glutathione - analysis</topic><topic>Immunity (Disease)</topic><topic>Immunologic Memory</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Oxidative Stress - physiology</topic><topic>Phenotype</topic><topic>Rodents</topic><topic>T-Lymphocytes - chemistry</topic><topic>T-Lymphocytes - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lohmiller, Jeffrey J.</creatorcontrib><creatorcontrib>Roellich, Kathleen M.</creatorcontrib><creatorcontrib>Toledano, Alicia</creatorcontrib><creatorcontrib>Rabinovitch, Peter S.</creatorcontrib><creatorcontrib>Wolf, Norman S.</creatorcontrib><creatorcontrib>Grossmann, Angelika</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Periodicals Index Online Segment 29</collection><collection>Periodicals Index Online</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - West</collection><collection>Primary Sources Access (Plan D) - International</collection><collection>Primary Sources Access & Build (Plan A) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Midwest</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Northeast</collection><collection>Primary Sources Access (Plan D) - Southeast</collection><collection>Primary Sources Access (Plan D) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Southeast</collection><collection>Primary Sources Access (Plan D) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - UK / I</collection><collection>Primary Sources Access (Plan D) - Canada</collection><collection>Primary Sources Access (Plan D) - EMEALA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - North Central</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - International</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - International</collection><collection>Primary Sources Access (Plan D) - West</collection><collection>Periodicals Index Online Segments 1-50</collection><collection>Primary Sources Access (Plan D) - APAC</collection><collection>Primary Sources Access (Plan D) - Midwest</collection><collection>Primary Sources Access (Plan D) - MEA</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - Canada</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - EMEALA</collection><collection>Primary Sources Access & Build (Plan A) - APAC</collection><collection>Primary Sources Access & Build (Plan A) - Canada</collection><collection>Primary Sources Access & Build (Plan A) - West</collection><collection>Primary Sources Access & Build (Plan A) - EMEALA</collection><collection>Primary Sources Access (Plan D) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - Midwest</collection><collection>Primary Sources Access & Build (Plan A) - North Central</collection><collection>Primary Sources Access & Build (Plan A) - Northeast</collection><collection>Primary Sources Access & Build (Plan A) - South Central</collection><collection>Primary Sources Access & Build (Plan A) - Southeast</collection><collection>Primary Sources Access (Plan D) - UK / I</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - APAC</collection><collection>Primary Sources Access—Foundation Edition (Plan E) - MEA</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>The journals of gerontology. Series A, Biological sciences and medical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lohmiller, Jeffrey J.</au><au>Roellich, Kathleen M.</au><au>Toledano, Alicia</au><au>Rabinovitch, Peter S.</au><au>Wolf, Norman S.</au><au>Grossmann, Angelika</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype</atitle><jtitle>The journals of gerontology. Series A, Biological sciences and medical sciences</jtitle><addtitle>J Gerontol A Biol Sci Med Sci</addtitle><date>1996-03-01</date><risdate>1996</risdate><volume>51A</volume><issue>2</issue><spage>B132</spage><epage>B140</epage><pages>B132-B140</pages><issn>1079-5006</issn><eissn>1758-535X</eissn><abstract>Glutathione (GSH) is the most important cytosolic antioxidant. Since GSH levels are decreased with age, we hypothesized that T-lymphocytes from old mice would be more sensitive to oxidative stress. T-lymphocytes from young and old mice were exposed to hypoxanthinelxanthine oxidase, and lymphocyte viability, proliferation, GSH content, and calcium signaling were measured. Before exposure, proliferation of T-lymphocytes from young mice was greater than that of old; following exposure, the converse was true. This was in spite of the fact that old mice had lower total GSH levels and greater levels of glutathione disulfide. After oxidative challenge, intracellular calcium responses to anti-CD3 were decreased in naive T-lymphocytes from all mice, while memory lymphocytes were less affected. Higher proportions of memory lymphocytes in old mice resulted in their greater overall preservation of lymphocyte function following oxidative injury, contrary to expectations that lower lymphocyte GSH content with age would increase susceptibility to oxidative stress.</abstract><cop>United States</cop><pub>The Gerontological Society of America</pub><pmid>8612097</pmid><doi>10.1093/gerona/51A.2.B132</doi><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1079-5006 |
ispartof | The journals of gerontology. Series A, Biological sciences and medical sciences, 1996-03, Vol.51A (2), p.B132-B140 |
issn | 1079-5006 1758-535X |
language | eng |
recordid | cdi_proquest_miscellaneous_78016021 |
source | MEDLINE; Periodicals Index Online; Oxford University Press Journals |
subjects | Aging Aging - physiology Animals Cell Survival Cellular biology Flow Cytometry Glutathione - analysis Immunity (Disease) Immunologic Memory Male Mice Mice, Inbred Strains Oxidative Stress - physiology Phenotype Rodents T-Lymphocytes - chemistry T-Lymphocytes - physiology |
title | Aged Murine T-lymphocytes Are More Resistant to Oxidative Damage Due to the Predominance of the Cells Possessing the Memory Phenotype |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-06T14%3A47%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Aged%20Murine%20T-lymphocytes%20Are%20More%20Resistant%20to%20Oxidative%20Damage%20Due%20to%20the%20Predominance%20of%20the%20Cells%20Possessing%20the%20Memory%20Phenotype&rft.jtitle=The%20journals%20of%20gerontology.%20Series%20A,%20Biological%20sciences%20and%20medical%20sciences&rft.au=Lohmiller,%20Jeffrey%20J.&rft.date=1996-03-01&rft.volume=51A&rft.issue=2&rft.spage=B132&rft.epage=B140&rft.pages=B132-B140&rft.issn=1079-5006&rft.eissn=1758-535X&rft_id=info:doi/10.1093/gerona/51A.2.B132&rft_dat=%3Cproquest_cross%3E1877135188%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1877135188&rft_id=info:pmid/8612097&rfr_iscdi=true |