Intrachromosomal localization of breakpoints induced by the restriction endonucleases AluI and BamHI in Chinese hamster ovary cells treated in S phase of the cell cycle
The restriction endonucleases (REs) AluI and BamHI were electroporated into Chinese hamster ovary (CHO) cells during S phase of the cell cycle and breakpoints in G-banded metaphases were mapped to Giemsa-light or -dark bands or to band junctions. The majority of AluI- and BamHI-induced breakpoints w...
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| Veröffentlicht in: | International journal of radiation biology 1996-04, Vol.69 (4), p.447-457 |
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| container_title | International journal of radiation biology |
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| creator | Folle, G A Obe, G |
| description | The restriction endonucleases (REs) AluI and BamHI were electroporated into Chinese hamster ovary (CHO) cells during S phase of the cell cycle and breakpoints in G-banded metaphases were mapped to Giemsa-light or -dark bands or to band junctions. The majority of AluI- and BamHI-induced breakpoints were located in Giemsa-light bands. Both REs induced similar distributions of breakpoint clusters. The localization pattern of S phase-induced breakpoints in CHO cells is similar to the pattern of G1-induced breakpoints reported earlier. These data show that breakpoint localization for both REs is independent of the cell cycle stage (G1 or S) in which aberrations are induced and give further support to the hypothesis that nuclease hypersensitive regions (NHRs) associated with active genes play an important role in the distribution of breakpoints. |
| doi_str_mv | 10.1080/095530096145742 |
| format | Article |
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The majority of AluI- and BamHI-induced breakpoints were located in Giemsa-light bands. Both REs induced similar distributions of breakpoint clusters. The localization pattern of S phase-induced breakpoints in CHO cells is similar to the pattern of G1-induced breakpoints reported earlier. These data show that breakpoint localization for both REs is independent of the cell cycle stage (G1 or S) in which aberrations are induced and give further support to the hypothesis that nuclease hypersensitive regions (NHRs) associated with active genes play an important role in the distribution of breakpoints.</description><identifier>ISSN: 0955-3002</identifier><identifier>DOI: 10.1080/095530096145742</identifier><identifier>PMID: 8627127</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; CHO Cells ; Chromosome Aberrations ; Chromosome Banding ; Cricetinae ; Deoxyribonuclease BamHI - pharmacology ; Deoxyribonucleases, Type II Site-Specific - pharmacology ; S Phase ; Space life sciences</subject><ispartof>International journal of radiation biology, 1996-04, Vol.69 (4), p.447-457</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8627127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Folle, G A</creatorcontrib><creatorcontrib>Obe, G</creatorcontrib><title>Intrachromosomal localization of breakpoints induced by the restriction endonucleases AluI and BamHI in Chinese hamster ovary cells treated in S phase of the cell cycle</title><title>International journal of radiation biology</title><addtitle>Int J Radiat Biol</addtitle><description>The restriction endonucleases (REs) AluI and BamHI were electroporated into Chinese hamster ovary (CHO) cells during S phase of the cell cycle and breakpoints in G-banded metaphases were mapped to Giemsa-light or -dark bands or to band junctions. The majority of AluI- and BamHI-induced breakpoints were located in Giemsa-light bands. Both REs induced similar distributions of breakpoint clusters. The localization pattern of S phase-induced breakpoints in CHO cells is similar to the pattern of G1-induced breakpoints reported earlier. These data show that breakpoint localization for both REs is independent of the cell cycle stage (G1 or S) in which aberrations are induced and give further support to the hypothesis that nuclease hypersensitive regions (NHRs) associated with active genes play an important role in the distribution of breakpoints.</description><subject>Animals</subject><subject>CHO Cells</subject><subject>Chromosome Aberrations</subject><subject>Chromosome Banding</subject><subject>Cricetinae</subject><subject>Deoxyribonuclease BamHI - pharmacology</subject><subject>Deoxyribonucleases, Type II Site-Specific - pharmacology</subject><subject>S Phase</subject><subject>Space life sciences</subject><issn>0955-3002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu2zAMhnVYkaVpzzsV4Gm3rJJlWfYxC9olQIEd1p0DWaJgb7bkSnKB7In6mJO3PEBPJMiP_0-ChHxi9AujNb2njRCc0qZipZBl8YGsl8o2l4qP5DrGXzRnlNcrsqqrQrJCrsnb0aWgdBf86KMf1QCD12ro_6jUewfeQhtQ_Z5871KE3plZo4H2DKlDCBhT6PU_Ep3xbtYDqogRdsN8BOUMfFXj4ZjnYN_1DiNCp8aYMIB_VeEMGochQsoWKctm7AdMXVZYjBeHpQ_6nGVvyJVVQ8TbS9yQn48Pz_vD9un7t-N-97SdCi7TtkVjWVtaYQsrdGWFsrzkhhZM1kw1lteUViXnRltTamO5qCQ2EgtkQrWy5hvy-b_uFPzLnA88jX1c1lAO_RxPsqasbN4BMlFLWkmWwbsLOLcjmtMU-jHffrr8gP8FMeOKQg</recordid><startdate>19960401</startdate><enddate>19960401</enddate><creator>Folle, G A</creator><creator>Obe, G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19960401</creationdate><title>Intrachromosomal localization of breakpoints induced by the restriction endonucleases AluI and BamHI in Chinese hamster ovary cells treated in S phase of the cell cycle</title><author>Folle, G A ; Obe, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p237t-bedf1b4f5f2f5c6f5af343d021781a9f38006433dcfd4cdf3567e97e2e15ab783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>CHO Cells</topic><topic>Chromosome Aberrations</topic><topic>Chromosome Banding</topic><topic>Cricetinae</topic><topic>Deoxyribonuclease BamHI - pharmacology</topic><topic>Deoxyribonucleases, Type II Site-Specific - pharmacology</topic><topic>S Phase</topic><topic>Space life sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Folle, G A</creatorcontrib><creatorcontrib>Obe, G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of radiation biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Folle, G A</au><au>Obe, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intrachromosomal localization of breakpoints induced by the restriction endonucleases AluI and BamHI in Chinese hamster ovary cells treated in S phase of the cell cycle</atitle><jtitle>International journal of radiation biology</jtitle><addtitle>Int J Radiat Biol</addtitle><date>1996-04-01</date><risdate>1996</risdate><volume>69</volume><issue>4</issue><spage>447</spage><epage>457</epage><pages>447-457</pages><issn>0955-3002</issn><abstract>The restriction endonucleases (REs) AluI and BamHI were electroporated into Chinese hamster ovary (CHO) cells during S phase of the cell cycle and breakpoints in G-banded metaphases were mapped to Giemsa-light or -dark bands or to band junctions. The majority of AluI- and BamHI-induced breakpoints were located in Giemsa-light bands. Both REs induced similar distributions of breakpoint clusters. The localization pattern of S phase-induced breakpoints in CHO cells is similar to the pattern of G1-induced breakpoints reported earlier. These data show that breakpoint localization for both REs is independent of the cell cycle stage (G1 or S) in which aberrations are induced and give further support to the hypothesis that nuclease hypersensitive regions (NHRs) associated with active genes play an important role in the distribution of breakpoints.</abstract><cop>England</cop><pmid>8627127</pmid><doi>10.1080/095530096145742</doi><tpages>11</tpages></addata></record> |
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| ispartof | International journal of radiation biology, 1996-04, Vol.69 (4), p.447-457 |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Animals CHO Cells Chromosome Aberrations Chromosome Banding Cricetinae Deoxyribonuclease BamHI - pharmacology Deoxyribonucleases, Type II Site-Specific - pharmacology S Phase Space life sciences |
| title | Intrachromosomal localization of breakpoints induced by the restriction endonucleases AluI and BamHI in Chinese hamster ovary cells treated in S phase of the cell cycle |
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