Multinucleated Giant Cell Formation of Swine Microglia Induced by Mycobacterium bovis
Multinucleated giant cells (MGC) have been long recognized as a histopathologic feature of tuberculosis, yet little is known about the underlying mechanism of tubercle bacillus-induced formation of these fused macrophages. The main purpose of this study was to characterize cellular mechanisms involv...
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| Veröffentlicht in: | The Journal of infectious diseases 1996-05, Vol.173 (5), p.1194-1201 |
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| container_title | The Journal of infectious diseases |
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| creator | Peterson, Phillip K. Gekker, Genya Hu, Shuxian Anderson, W. Robert Teichert, Matthew Chao, Chun C. Molitor, Thomas W. |
| description | Multinucleated giant cells (MGC) have been long recognized as a histopathologic feature of tuberculosis, yet little is known about the underlying mechanism of tubercle bacillus-induced formation of these fused macrophages. The main purpose of this study was to characterize cellular mechanisms involved in MGC formation of swine microglia, the resident macrophages of the brain, in cultures containing nonopsonized Mycobacterium bovis. Within 2 h of incubation, MGC were readily detected in these cultures by light and transmission electron microscopy. MGC formation was blocked by anti-C014 and anti-C018 antibodies and by thalidomide, a potent inhibitor of tumor necrosis factor-α (TNF-α) production by microglia. Also, TNF-α alone induced MGC formation. These findings suggest that two microglial cell receptors, CD14 and a β2 integrin, and the cytokine TNF-α participate in M. bovis-induced swine microglial MGC formation. |
| doi_str_mv | 10.1093/infdis/173.5.1194 |
| format | Article |
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Robert ; Teichert, Matthew ; Chao, Chun C. ; Molitor, Thomas W.</creator><creatorcontrib>Peterson, Phillip K. ; Gekker, Genya ; Hu, Shuxian ; Anderson, W. Robert ; Teichert, Matthew ; Chao, Chun C. ; Molitor, Thomas W.</creatorcontrib><description>Multinucleated giant cells (MGC) have been long recognized as a histopathologic feature of tuberculosis, yet little is known about the underlying mechanism of tubercle bacillus-induced formation of these fused macrophages. The main purpose of this study was to characterize cellular mechanisms involved in MGC formation of swine microglia, the resident macrophages of the brain, in cultures containing nonopsonized Mycobacterium bovis. Within 2 h of incubation, MGC were readily detected in these cultures by light and transmission electron microscopy. MGC formation was blocked by anti-C014 and anti-C018 antibodies and by thalidomide, a potent inhibitor of tumor necrosis factor-α (TNF-α) production by microglia. Also, TNF-α alone induced MGC formation. These findings suggest that two microglial cell receptors, CD14 and a β2 integrin, and the cytokine TNF-α participate in M. bovis-induced swine microglial MGC formation.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/173.5.1194</identifier><identifier>PMID: 8627072</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Animals ; Antibodies ; Bacterial diseases ; Biological and medical sciences ; CD18 Antigens - physiology ; Cell culture techniques ; Cells, Cultured ; Cultured cells ; Giant cells ; Giant Cells - cytology ; Giant Cells - microbiology ; Giant Cells - ultrastructure ; Human bacterial diseases ; Infectious diseases ; Integrins ; Lipopolysaccharide Receptors - physiology ; Macrophages ; Major Articles ; Medical sciences ; Microglia ; Microglia - cytology ; Microglia - drug effects ; Microglia - metabolism ; Monoclonal antibodies ; Mycobacterium bovis ; Mycobacterium bovis - pathogenicity ; Mycobacterium bovis - physiology ; Mycobacterium tuberculosis ; Receptors ; Swine ; Thalidomide - pharmacology ; Tuberculosis and atypical mycobacterial infections ; Tumor Necrosis Factor-alpha - pharmacology ; Tumor Necrosis Factor-alpha - physiology ; Virulence</subject><ispartof>The Journal of infectious diseases, 1996-05, Vol.173 (5), p.1194-1201</ispartof><rights>Copyright 1996 The University of Chicago</rights><rights>1996 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press May 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c576t-d2a23567575f64bb643de8c86374e0c325d5faf1ade17644e147e3332206a6dc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30123021$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30123021$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3055778$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8627072$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peterson, Phillip K.</creatorcontrib><creatorcontrib>Gekker, Genya</creatorcontrib><creatorcontrib>Hu, Shuxian</creatorcontrib><creatorcontrib>Anderson, W. Robert</creatorcontrib><creatorcontrib>Teichert, Matthew</creatorcontrib><creatorcontrib>Chao, Chun C.</creatorcontrib><creatorcontrib>Molitor, Thomas W.</creatorcontrib><title>Multinucleated Giant Cell Formation of Swine Microglia Induced by Mycobacterium bovis</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Multinucleated giant cells (MGC) have been long recognized as a histopathologic feature of tuberculosis, yet little is known about the underlying mechanism of tubercle bacillus-induced formation of these fused macrophages. The main purpose of this study was to characterize cellular mechanisms involved in MGC formation of swine microglia, the resident macrophages of the brain, in cultures containing nonopsonized Mycobacterium bovis. Within 2 h of incubation, MGC were readily detected in these cultures by light and transmission electron microscopy. MGC formation was blocked by anti-C014 and anti-C018 antibodies and by thalidomide, a potent inhibitor of tumor necrosis factor-α (TNF-α) production by microglia. Also, TNF-α alone induced MGC formation. These findings suggest that two microglial cell receptors, CD14 and a β2 integrin, and the cytokine TNF-α participate in M. bovis-induced swine microglial MGC formation.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Bacterial diseases</subject><subject>Biological and medical sciences</subject><subject>CD18 Antigens - physiology</subject><subject>Cell culture techniques</subject><subject>Cells, Cultured</subject><subject>Cultured cells</subject><subject>Giant cells</subject><subject>Giant Cells - cytology</subject><subject>Giant Cells - microbiology</subject><subject>Giant Cells - ultrastructure</subject><subject>Human bacterial diseases</subject><subject>Infectious diseases</subject><subject>Integrins</subject><subject>Lipopolysaccharide Receptors - physiology</subject><subject>Macrophages</subject><subject>Major Articles</subject><subject>Medical sciences</subject><subject>Microglia</subject><subject>Microglia - cytology</subject><subject>Microglia - drug effects</subject><subject>Microglia - metabolism</subject><subject>Monoclonal antibodies</subject><subject>Mycobacterium bovis</subject><subject>Mycobacterium bovis - pathogenicity</subject><subject>Mycobacterium bovis - physiology</subject><subject>Mycobacterium tuberculosis</subject><subject>Receptors</subject><subject>Swine</subject><subject>Thalidomide - pharmacology</subject><subject>Tuberculosis and atypical mycobacterial infections</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><subject>Tumor Necrosis Factor-alpha - physiology</subject><subject>Virulence</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhi0EKiHwAzggrRDitqnH44_dIwo0bdXAAVohLpbX60UOm3Vr7wL59zhKKIgLJx_eZ16N5yHkOdAF0BpP_dC1Pp2CwoVYANT8AZmBQFVKCfiQzChlrISqrh-TJyltKKUcpTohJ5Vkiio2I9frqR_9MNnemdG1xcqbYSyWru-LsxC3ZvRhKEJXfPzhB1esvY3ha-9NcTG0k818syvWOxsaY0cX_bQtmvDdp6fkUWf65J4d3zm5Pnv3aXleXn1YXSzfXJVWKDmWLTMMhVRCiU7yppEcW1fZSqLijlpkohWd6cC0DpTk3AFXDhEZo9LI1uKcvD703sZwN7k06q1PNi9vBhempFVFAZTC_4IgJHDGWQZf_gNuwhSH_AnNGNYMsN63wQHK10gpuk7fRr81caeB6r0YfRCjsxgt9F5MnnlxLJ6arWvvJ44mcv7qmJtkTd9FM9jc8BtDKoRS1Z-aTRpD_CsGhjRvNyflIfdpdD_vcxO_aalQCX3--Ytevb25fF_fgL7EX7Z8rsg</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>Peterson, Phillip K.</creator><creator>Gekker, Genya</creator><creator>Hu, Shuxian</creator><creator>Anderson, W. Robert</creator><creator>Teichert, Matthew</creator><creator>Chao, Chun C.</creator><creator>Molitor, Thomas W.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7QL</scope><scope>7TK</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19960501</creationdate><title>Multinucleated Giant Cell Formation of Swine Microglia Induced by Mycobacterium bovis</title><author>Peterson, Phillip K. ; Gekker, Genya ; Hu, Shuxian ; Anderson, W. Robert ; Teichert, Matthew ; Chao, Chun C. ; Molitor, Thomas W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c576t-d2a23567575f64bb643de8c86374e0c325d5faf1ade17644e147e3332206a6dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Bacterial diseases</topic><topic>Biological and medical sciences</topic><topic>CD18 Antigens - physiology</topic><topic>Cell culture techniques</topic><topic>Cells, Cultured</topic><topic>Cultured cells</topic><topic>Giant cells</topic><topic>Giant Cells - cytology</topic><topic>Giant Cells - microbiology</topic><topic>Giant Cells - ultrastructure</topic><topic>Human bacterial diseases</topic><topic>Infectious diseases</topic><topic>Integrins</topic><topic>Lipopolysaccharide Receptors - physiology</topic><topic>Macrophages</topic><topic>Major Articles</topic><topic>Medical sciences</topic><topic>Microglia</topic><topic>Microglia - cytology</topic><topic>Microglia - drug effects</topic><topic>Microglia - metabolism</topic><topic>Monoclonal antibodies</topic><topic>Mycobacterium bovis</topic><topic>Mycobacterium bovis - pathogenicity</topic><topic>Mycobacterium bovis - physiology</topic><topic>Mycobacterium tuberculosis</topic><topic>Receptors</topic><topic>Swine</topic><topic>Thalidomide - pharmacology</topic><topic>Tuberculosis and atypical mycobacterial infections</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><topic>Tumor Necrosis Factor-alpha - physiology</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peterson, Phillip K.</creatorcontrib><creatorcontrib>Gekker, Genya</creatorcontrib><creatorcontrib>Hu, Shuxian</creatorcontrib><creatorcontrib>Anderson, W. Robert</creatorcontrib><creatorcontrib>Teichert, Matthew</creatorcontrib><creatorcontrib>Chao, Chun C.</creatorcontrib><creatorcontrib>Molitor, Thomas W.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Neurosciences Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peterson, Phillip K.</au><au>Gekker, Genya</au><au>Hu, Shuxian</au><au>Anderson, W. Robert</au><au>Teichert, Matthew</au><au>Chao, Chun C.</au><au>Molitor, Thomas W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multinucleated Giant Cell Formation of Swine Microglia Induced by Mycobacterium bovis</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>173</volume><issue>5</issue><spage>1194</spage><epage>1201</epage><pages>1194-1201</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Multinucleated giant cells (MGC) have been long recognized as a histopathologic feature of tuberculosis, yet little is known about the underlying mechanism of tubercle bacillus-induced formation of these fused macrophages. The main purpose of this study was to characterize cellular mechanisms involved in MGC formation of swine microglia, the resident macrophages of the brain, in cultures containing nonopsonized Mycobacterium bovis. 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| issn | 0022-1899 1537-6613 |
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| source | MEDLINE; JSTOR; Oxford Journals |
| subjects | Animals Antibodies Bacterial diseases Biological and medical sciences CD18 Antigens - physiology Cell culture techniques Cells, Cultured Cultured cells Giant cells Giant Cells - cytology Giant Cells - microbiology Giant Cells - ultrastructure Human bacterial diseases Infectious diseases Integrins Lipopolysaccharide Receptors - physiology Macrophages Major Articles Medical sciences Microglia Microglia - cytology Microglia - drug effects Microglia - metabolism Monoclonal antibodies Mycobacterium bovis Mycobacterium bovis - pathogenicity Mycobacterium bovis - physiology Mycobacterium tuberculosis Receptors Swine Thalidomide - pharmacology Tuberculosis and atypical mycobacterial infections Tumor Necrosis Factor-alpha - pharmacology Tumor Necrosis Factor-alpha - physiology Virulence |
| title | Multinucleated Giant Cell Formation of Swine Microglia Induced by Mycobacterium bovis |
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