Heteronuclear relayed E.COSY applied to the determination of accurate 3J(HN,C') and 3J(H beta,C') coupling constants in desulfovibrio vulgaris flavodoxin

A simple constant-time 3D heteronuclear NMR pulse sequence has been developed to quantitatively determine the heteronuclear three-bond couplings 3J(HN,C') and 3J(H beta,C') in uniformly 13C-enriched proteins. The protocols for measuring accurate coupling constants are based on 1H,13C-heter...

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Veröffentlicht in:	Journal of biomolecular NMR 1996-03, Vol.7 (2), p.142-152
Hauptverfasser:	Schmidt, J M, Löhr, F, Rüterjans, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Computer Simulation Desulfovibrio vulgaris - chemistry Flavodoxin - chemistry Flavodoxin - genetics Magnetic Resonance Spectroscopy - methods Models, Chemical Molecular Sequence Data Normal Distribution Recombinant Proteins - chemistry Reproducibility of Results Solutions
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