NMR evidence for helix geometry modifications by a G-U wobble base pair in the acceptor arm of E. coli tRNA(Ala)
A ribooligonucleotide duplex representing the acceptor stem of E. coli RNA(Ala) with a G3-U70 wobble base pair, which is the main identity element for the recognition by the alanine-tRNA synthetase, has been characterized by 2D-NMR, as having two sequence variants with a regular Watson-Crick G3-C70...
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| Veröffentlicht in: | FEBS letters 1996-04, Vol.385 (1-2), p.15-20 |
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| creator | Limmer, S Reif, B Ott, G Arnold, L Sprinzl, M |
| description | A ribooligonucleotide duplex representing the acceptor stem of E. coli RNA(Ala) with a G3-U70 wobble base pair, which is the main identity element for the recognition by the alanine-tRNA synthetase, has been characterized by 2D-NMR, as having two sequence variants with a regular Watson-Crick G3-C70 and an I3-U70 wobble pair, respectively. As compared to a regular A-RNA, the G-U base pair gives rise to variations of the local helix geometry which are reflected in distinct local chemical shift changes. Structural differences between the duplex possessing an I3-U70 base pair and the wild-type G3-U70 sequence have also been found. The nucleotides in the ubiquitous single-stranded NCCA terminus display a surprisingly high degree of stacking order, especially between A73, C74, and C75. |
| doi_str_mv | 10.1016/0014-5793(96)00339-0 |
| format | Article |
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As compared to a regular A-RNA, the G-U base pair gives rise to variations of the local helix geometry which are reflected in distinct local chemical shift changes. Structural differences between the duplex possessing an I3-U70 base pair and the wild-type G3-U70 sequence have also been found. 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As compared to a regular A-RNA, the G-U base pair gives rise to variations of the local helix geometry which are reflected in distinct local chemical shift changes. Structural differences between the duplex possessing an I3-U70 base pair and the wild-type G3-U70 sequence have also been found. The nucleotides in the ubiquitous single-stranded NCCA terminus display a surprisingly high degree of stacking order, especially between A73, C74, and C75.</description><subject>Base Composition</subject><subject>Base Sequence</subject><subject>Escherichia coli - chemistry</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Oligoribonucleotides - chemical synthesis</subject><subject>Oligoribonucleotides - chemistry</subject><subject>Point Mutation</subject><subject>RNA, Bacterial - chemistry</subject><subject>RNA, Bacterial - genetics</subject><subject>RNA, Transfer, Ala - chemistry</subject><subject>RNA, Transfer, Ala - genetics</subject><issn>0014-5793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UE1PwjAY7kGDiP4DTd6TgcPwbTs6diQG0QQxIXJe2u2t1GzrXIfKv3eJxNPzmefwMHbDccqRq3tEHkezJJXjVE0QpUwjPGPDf_uCXYbwgb2e83TABnMV83iWDFmzedkCfbmC6pzA-hb2VLofeCdfUdceofKFsy7XnfN1AHMEDatoB9_emJLA6EDQaNeCq6HbE-g8p6brZ3RbgbewnELuSwfddrMYL0o9uWLnVpeBrk84YrvH5dvDU7R-XT0_LNZRI1B1EVexRKUMykQqa63gqSA-N1r0VHIyVgujcqmFSPpccWM4WaGkkrKwZOWI3f3tNq3_PFDossqFnMpS1-QPIUvmiAI59sXbU_FgKiqypnWVbo_Z6SL5C8f6ZbA</recordid><startdate>19960429</startdate><enddate>19960429</enddate><creator>Limmer, S</creator><creator>Reif, B</creator><creator>Ott, G</creator><creator>Arnold, L</creator><creator>Sprinzl, M</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19960429</creationdate><title>NMR evidence for helix geometry modifications by a G-U wobble base pair in the acceptor arm of E. coli tRNA(Ala)</title><author>Limmer, S ; Reif, B ; Ott, G ; Arnold, L ; Sprinzl, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p206t-1643066b03736fff2192e18ba2f2131ebfa2b6c3a2276ff61bb1ef263633dfef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Base Composition</topic><topic>Base Sequence</topic><topic>Escherichia coli - chemistry</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Oligoribonucleotides - chemical synthesis</topic><topic>Oligoribonucleotides - chemistry</topic><topic>Point Mutation</topic><topic>RNA, Bacterial - chemistry</topic><topic>RNA, Bacterial - genetics</topic><topic>RNA, Transfer, Ala - chemistry</topic><topic>RNA, Transfer, Ala - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Limmer, S</creatorcontrib><creatorcontrib>Reif, B</creatorcontrib><creatorcontrib>Ott, G</creatorcontrib><creatorcontrib>Arnold, L</creatorcontrib><creatorcontrib>Sprinzl, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Limmer, S</au><au>Reif, B</au><au>Ott, G</au><au>Arnold, L</au><au>Sprinzl, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NMR evidence for helix geometry modifications by a G-U wobble base pair in the acceptor arm of E. coli tRNA(Ala)</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1996-04-29</date><risdate>1996</risdate><volume>385</volume><issue>1-2</issue><spage>15</spage><epage>20</epage><pages>15-20</pages><issn>0014-5793</issn><abstract>A ribooligonucleotide duplex representing the acceptor stem of E. coli RNA(Ala) with a G3-U70 wobble base pair, which is the main identity element for the recognition by the alanine-tRNA synthetase, has been characterized by 2D-NMR, as having two sequence variants with a regular Watson-Crick G3-C70 and an I3-U70 wobble pair, respectively. As compared to a regular A-RNA, the G-U base pair gives rise to variations of the local helix geometry which are reflected in distinct local chemical shift changes. Structural differences between the duplex possessing an I3-U70 base pair and the wild-type G3-U70 sequence have also been found. The nucleotides in the ubiquitous single-stranded NCCA terminus display a surprisingly high degree of stacking order, especially between A73, C74, and C75.</abstract><cop>England</cop><pmid>8641457</pmid><doi>10.1016/0014-5793(96)00339-0</doi><tpages>6</tpages></addata></record> |
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| ispartof | FEBS letters, 1996-04, Vol.385 (1-2), p.15-20 |
| issn | 0014-5793 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Base Composition Base Sequence Escherichia coli - chemistry Magnetic Resonance Spectroscopy Molecular Sequence Data Nucleic Acid Conformation Oligoribonucleotides - chemical synthesis Oligoribonucleotides - chemistry Point Mutation RNA, Bacterial - chemistry RNA, Bacterial - genetics RNA, Transfer, Ala - chemistry RNA, Transfer, Ala - genetics |
| title | NMR evidence for helix geometry modifications by a G-U wobble base pair in the acceptor arm of E. coli tRNA(Ala) |
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