Induction of specific allograft immunity by soluble class I MHC heavy chain protein produced in a baculovirus expression system
Spodoptera frugiperda (Sf9) insect cells secreted a class I MHC RT1.Aa heavy chain protein when infected with baculovirus that bore a construct that contained a honeybee melittin secretion (ms) signal attached to RT1.Aa cDNA. The RT1.Aa heavy chain protein in the culture supernatant and cell lysate...
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| Veröffentlicht in: | Transplantation 1996-02, Vol.61 (3), p.448-457 |
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| creator | Wang, M Stepkowski, S M Wang, M E Tian, L Qu, X Tu, Y He, G Kahan, B D |
| description | Spodoptera frugiperda (Sf9) insect cells secreted a class I MHC RT1.Aa heavy chain protein when infected with baculovirus that bore a construct that contained a honeybee melittin secretion (ms) signal attached to RT1.Aa cDNA. The RT1.Aa heavy chain protein in the culture supernatant and cell lysate immunoprecipitated in the presence of 5 individual anti-RT1.Aa-specific mAb. As was revealed by densitometric analysis, the ms signal increased the production (7- to 17-fold) and secretion (20- to 47-fold) of RT1.Aa protein by Sf9 cells (compared with RT1Aa-Sf9 cells without the ms signal). Subcutaneous immunization with secreted RT1.Aa heavy chain protein of Wistar-Furth (WF; RT1u) rats (day -4) accelerated the rejection of ACI (RT1a), but not third-party Brown Norway (BN; RT1n), heart allografts from 5.9 +/- 0.5 days in controls to 4.0 +/- 0.0 days (P < 0.001); cell lysate from RT1.Aa-Sf9 or ms/RT1.Aa-Sf9 cells reduced ACI heart allograft survival to 3.8 +/- 0.4 days or 3.7 +/- 0.5 days, respectively (P < 0.001). Indirect presentation of RT1.Aa heavy chain proteins by syngeneic macrophages shortened the survival of RT1.Aa-disparate PVG.R8 (RT1.AaDuBuCu) heart allografts in PVG.1U (RT1u) hosts from 6.3 +/- 0.5 days in controls to 4.0 +/- 0.0 days (P < 0.01). Finally, RT1.Aa heavy chain proteins injected into the thymus or into the portal vein (day -14) in combination with anti-T cell receptor mAb (days -14 and -13) induced indefinite survival of ACI liver allografts in Lewis (RT1l) recipients ( > 250 days). Thus, indirect presentation of soluble class I MHC heavy chain proteins (produced in a baculovirus/Sf9 cell system) may either sensitize or induce tolerance in the same fashion as native class I MHC alloantigens expressed on donor tissues. |
| doi_str_mv | 10.1097/00007890-199602150-00024 |
| format | Article |
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The RT1.Aa heavy chain protein in the culture supernatant and cell lysate immunoprecipitated in the presence of 5 individual anti-RT1.Aa-specific mAb. As was revealed by densitometric analysis, the ms signal increased the production (7- to 17-fold) and secretion (20- to 47-fold) of RT1.Aa protein by Sf9 cells (compared with RT1Aa-Sf9 cells without the ms signal). Subcutaneous immunization with secreted RT1.Aa heavy chain protein of Wistar-Furth (WF; RT1u) rats (day -4) accelerated the rejection of ACI (RT1a), but not third-party Brown Norway (BN; RT1n), heart allografts from 5.9 +/- 0.5 days in controls to 4.0 +/- 0.0 days (P < 0.001); cell lysate from RT1.Aa-Sf9 or ms/RT1.Aa-Sf9 cells reduced ACI heart allograft survival to 3.8 +/- 0.4 days or 3.7 +/- 0.5 days, respectively (P < 0.001). Indirect presentation of RT1.Aa heavy chain proteins by syngeneic macrophages shortened the survival of RT1.Aa-disparate PVG.R8 (RT1.AaDuBuCu) heart allografts in PVG.1U (RT1u) hosts from 6.3 +/- 0.5 days in controls to 4.0 +/- 0.0 days (P < 0.01). Finally, RT1.Aa heavy chain proteins injected into the thymus or into the portal vein (day -14) in combination with anti-T cell receptor mAb (days -14 and -13) induced indefinite survival of ACI liver allografts in Lewis (RT1l) recipients ( > 250 days). Thus, indirect presentation of soluble class I MHC heavy chain proteins (produced in a baculovirus/Sf9 cell system) may either sensitize or induce tolerance in the same fashion as native class I MHC alloantigens expressed on donor tissues.</description><identifier>ISSN: 0041-1337</identifier><identifier>DOI: 10.1097/00007890-199602150-00024</identifier><identifier>PMID: 8610360</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Antigen Presentation ; Baculoviridae - genetics ; baculovirus ; Base Sequence ; Cell Line ; DNA Primers - genetics ; Histocompatibility Antigens Class I - administration & dosage ; Histocompatibility Antigens Class I - biosynthesis ; Histocompatibility Antigens Class I - genetics ; Immune Tolerance ; Immunization ; Injections, Subcutaneous ; Liver Transplantation - immunology ; Male ; Melitten - genetics ; Molecular Sequence Data ; Rats ; Rats, Inbred ACI ; Rats, Inbred BN ; Rats, Inbred Lew ; Rats, Inbred WF ; Solubility ; Spodoptera ; Spodoptera frugiperda ; Transplantation, Homologous</subject><ispartof>Transplantation, 1996-02, Vol.61 (3), p.448-457</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8610360$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, M</creatorcontrib><creatorcontrib>Stepkowski, S M</creatorcontrib><creatorcontrib>Wang, M E</creatorcontrib><creatorcontrib>Tian, L</creatorcontrib><creatorcontrib>Qu, X</creatorcontrib><creatorcontrib>Tu, Y</creatorcontrib><creatorcontrib>He, G</creatorcontrib><creatorcontrib>Kahan, B D</creatorcontrib><title>Induction of specific allograft immunity by soluble class I MHC heavy chain protein produced in a baculovirus expression system</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Spodoptera frugiperda (Sf9) insect cells secreted a class I MHC RT1.Aa heavy chain protein when infected with baculovirus that bore a construct that contained a honeybee melittin secretion (ms) signal attached to RT1.Aa cDNA. The RT1.Aa heavy chain protein in the culture supernatant and cell lysate immunoprecipitated in the presence of 5 individual anti-RT1.Aa-specific mAb. As was revealed by densitometric analysis, the ms signal increased the production (7- to 17-fold) and secretion (20- to 47-fold) of RT1.Aa protein by Sf9 cells (compared with RT1Aa-Sf9 cells without the ms signal). Subcutaneous immunization with secreted RT1.Aa heavy chain protein of Wistar-Furth (WF; RT1u) rats (day -4) accelerated the rejection of ACI (RT1a), but not third-party Brown Norway (BN; RT1n), heart allografts from 5.9 +/- 0.5 days in controls to 4.0 +/- 0.0 days (P < 0.001); cell lysate from RT1.Aa-Sf9 or ms/RT1.Aa-Sf9 cells reduced ACI heart allograft survival to 3.8 +/- 0.4 days or 3.7 +/- 0.5 days, respectively (P < 0.001). Indirect presentation of RT1.Aa heavy chain proteins by syngeneic macrophages shortened the survival of RT1.Aa-disparate PVG.R8 (RT1.AaDuBuCu) heart allografts in PVG.1U (RT1u) hosts from 6.3 +/- 0.5 days in controls to 4.0 +/- 0.0 days (P < 0.01). Finally, RT1.Aa heavy chain proteins injected into the thymus or into the portal vein (day -14) in combination with anti-T cell receptor mAb (days -14 and -13) induced indefinite survival of ACI liver allografts in Lewis (RT1l) recipients ( > 250 days). Thus, indirect presentation of soluble class I MHC heavy chain proteins (produced in a baculovirus/Sf9 cell system) may either sensitize or induce tolerance in the same fashion as native class I MHC alloantigens expressed on donor tissues.</description><subject>Animals</subject><subject>Antigen Presentation</subject><subject>Baculoviridae - genetics</subject><subject>baculovirus</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>DNA Primers - genetics</subject><subject>Histocompatibility Antigens Class I - administration & dosage</subject><subject>Histocompatibility Antigens Class I - biosynthesis</subject><subject>Histocompatibility Antigens Class I - genetics</subject><subject>Immune Tolerance</subject><subject>Immunization</subject><subject>Injections, Subcutaneous</subject><subject>Liver Transplantation - immunology</subject><subject>Male</subject><subject>Melitten - genetics</subject><subject>Molecular Sequence Data</subject><subject>Rats</subject><subject>Rats, Inbred ACI</subject><subject>Rats, Inbred BN</subject><subject>Rats, Inbred Lew</subject><subject>Rats, Inbred WF</subject><subject>Solubility</subject><subject>Spodoptera</subject><subject>Spodoptera frugiperda</subject><subject>Transplantation, Homologous</subject><issn>0041-1337</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUD1PwzAQ9QAqpfATkDyxBfyV2B5RBbRSEQvMke1cqCsnKXFckYm_Tqp255Z3enr33t0hhCl5oETLRzKVVJpkVOuCMJqTbGKYuEBzQgTNKOfyCl3HuJvonEs5QzNVUMILMke_67ZKbvBdi7saxz04X3uHTQjdV2_qAfumSa0fRmxHHLuQbADsgokRr_Hbaom3YA4jdlvjW7zvuwFOOJlChafeYGtcCt3B9yli-Nn3EOMxLo5xgOYGXdYmRLg94wJ9vjx_LFfZ5v11vXzaZDvO2ZA5bRQYoywTynEhRC1yablweQ3cWGGYnI6vtaVQ66JglhEDVGitFaOkyvkC3Z98p92-E8ShbHx0EIJpoUuxlNO8Ukz-K6R5QRTnR8e7szDZBqpy3_vG9GN5fi3_A-OjfHw</recordid><startdate>19960215</startdate><enddate>19960215</enddate><creator>Wang, M</creator><creator>Stepkowski, S M</creator><creator>Wang, M E</creator><creator>Tian, L</creator><creator>Qu, X</creator><creator>Tu, Y</creator><creator>He, G</creator><creator>Kahan, B D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19960215</creationdate><title>Induction of specific allograft immunity by soluble class I MHC heavy chain protein produced in a baculovirus expression system</title><author>Wang, M ; Stepkowski, S M ; Wang, M E ; Tian, L ; Qu, X ; Tu, Y ; He, G ; Kahan, B D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j332t-c9a8eaa8b248c3444f457b34c5fe3ab4a27996f9b1ef9662b20ae149998210d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antigen Presentation</topic><topic>Baculoviridae - genetics</topic><topic>baculovirus</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>DNA Primers - genetics</topic><topic>Histocompatibility Antigens Class I - administration & dosage</topic><topic>Histocompatibility Antigens Class I - biosynthesis</topic><topic>Histocompatibility Antigens Class I - genetics</topic><topic>Immune Tolerance</topic><topic>Immunization</topic><topic>Injections, Subcutaneous</topic><topic>Liver Transplantation - immunology</topic><topic>Male</topic><topic>Melitten - genetics</topic><topic>Molecular Sequence Data</topic><topic>Rats</topic><topic>Rats, Inbred ACI</topic><topic>Rats, Inbred BN</topic><topic>Rats, Inbred Lew</topic><topic>Rats, Inbred WF</topic><topic>Solubility</topic><topic>Spodoptera</topic><topic>Spodoptera frugiperda</topic><topic>Transplantation, Homologous</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, M</creatorcontrib><creatorcontrib>Stepkowski, S M</creatorcontrib><creatorcontrib>Wang, M E</creatorcontrib><creatorcontrib>Tian, L</creatorcontrib><creatorcontrib>Qu, X</creatorcontrib><creatorcontrib>Tu, Y</creatorcontrib><creatorcontrib>He, G</creatorcontrib><creatorcontrib>Kahan, B D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, M</au><au>Stepkowski, S M</au><au>Wang, M E</au><au>Tian, L</au><au>Qu, X</au><au>Tu, Y</au><au>He, G</au><au>Kahan, B D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of specific allograft immunity by soluble class I MHC heavy chain protein produced in a baculovirus expression system</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>1996-02-15</date><risdate>1996</risdate><volume>61</volume><issue>3</issue><spage>448</spage><epage>457</epage><pages>448-457</pages><issn>0041-1337</issn><abstract>Spodoptera frugiperda (Sf9) insect cells secreted a class I MHC RT1.Aa heavy chain protein when infected with baculovirus that bore a construct that contained a honeybee melittin secretion (ms) signal attached to RT1.Aa cDNA. The RT1.Aa heavy chain protein in the culture supernatant and cell lysate immunoprecipitated in the presence of 5 individual anti-RT1.Aa-specific mAb. As was revealed by densitometric analysis, the ms signal increased the production (7- to 17-fold) and secretion (20- to 47-fold) of RT1.Aa protein by Sf9 cells (compared with RT1Aa-Sf9 cells without the ms signal). Subcutaneous immunization with secreted RT1.Aa heavy chain protein of Wistar-Furth (WF; RT1u) rats (day -4) accelerated the rejection of ACI (RT1a), but not third-party Brown Norway (BN; RT1n), heart allografts from 5.9 +/- 0.5 days in controls to 4.0 +/- 0.0 days (P < 0.001); cell lysate from RT1.Aa-Sf9 or ms/RT1.Aa-Sf9 cells reduced ACI heart allograft survival to 3.8 +/- 0.4 days or 3.7 +/- 0.5 days, respectively (P < 0.001). Indirect presentation of RT1.Aa heavy chain proteins by syngeneic macrophages shortened the survival of RT1.Aa-disparate PVG.R8 (RT1.AaDuBuCu) heart allografts in PVG.1U (RT1u) hosts from 6.3 +/- 0.5 days in controls to 4.0 +/- 0.0 days (P < 0.01). Finally, RT1.Aa heavy chain proteins injected into the thymus or into the portal vein (day -14) in combination with anti-T cell receptor mAb (days -14 and -13) induced indefinite survival of ACI liver allografts in Lewis (RT1l) recipients ( > 250 days). Thus, indirect presentation of soluble class I MHC heavy chain proteins (produced in a baculovirus/Sf9 cell system) may either sensitize or induce tolerance in the same fashion as native class I MHC alloantigens expressed on donor tissues.</abstract><cop>United States</cop><pmid>8610360</pmid><doi>10.1097/00007890-199602150-00024</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0041-1337 |
| ispartof | Transplantation, 1996-02, Vol.61 (3), p.448-457 |
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| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Animals Antigen Presentation Baculoviridae - genetics baculovirus Base Sequence Cell Line DNA Primers - genetics Histocompatibility Antigens Class I - administration & dosage Histocompatibility Antigens Class I - biosynthesis Histocompatibility Antigens Class I - genetics Immune Tolerance Immunization Injections, Subcutaneous Liver Transplantation - immunology Male Melitten - genetics Molecular Sequence Data Rats Rats, Inbred ACI Rats, Inbred BN Rats, Inbred Lew Rats, Inbred WF Solubility Spodoptera Spodoptera frugiperda Transplantation, Homologous |
| title | Induction of specific allograft immunity by soluble class I MHC heavy chain protein produced in a baculovirus expression system |
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