Increased Expression of Insulin Receptor Substrate-1 in Human Pancreatic Cancer
Insulin receptor substrate-1 (IRS-1) is a multisite docking protein implicated in mitogenic signaling following activation of the insulin and insulin-like growth factor I receptors. In the present study we characterized IRS-1 expression in human pancreatic cancer. Northern blot analysis revealed hig...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1996-03, Vol.220 (3), p.886-890 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Bergmann, Uwe Funatomi, Hitoshi Kornmann, Marko Beger, Hans G. Korc, Murray |
| description | Insulin receptor substrate-1 (IRS-1) is a multisite docking protein implicated in mitogenic signaling following activation of the insulin and insulin-like growth factor I receptors. In the present study we characterized IRS-1 expression in human pancreatic cancer. Northern blot analysis revealed high levels of IRS-1 mRNA transcripts in ASPC-1 and MIA PaCa-2 human pancreatic cancer cell lines, and lower levels in COLO-357, PANC-1, and T3M4 cells. Immunoblotting with anti-IRS-1 antibodies indicated that IRS-1 protein levels paralleled IRS-1 mRNA levels. Analysis of RNA isolated from normal and cancerous human pancreatic tissues indicated that 7 of 16 pancreatic cancer samples overexpressed IRS-1 mRNA transcripts by comparison with the normal pancreas and that insulin mRNA levels were abundant in many tumors. These data suggest that IRS-1 contributes to the signaling pathways that lead to excessive growth stimulation in human pancreatic cancer. |
| doi_str_mv | 10.1006/bbrc.1996.0500 |
| format | Article |
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In the present study we characterized IRS-1 expression in human pancreatic cancer. Northern blot analysis revealed high levels of IRS-1 mRNA transcripts in ASPC-1 and MIA PaCa-2 human pancreatic cancer cell lines, and lower levels in COLO-357, PANC-1, and T3M4 cells. Immunoblotting with anti-IRS-1 antibodies indicated that IRS-1 protein levels paralleled IRS-1 mRNA levels. Analysis of RNA isolated from normal and cancerous human pancreatic tissues indicated that 7 of 16 pancreatic cancer samples overexpressed IRS-1 mRNA transcripts by comparison with the normal pancreas and that insulin mRNA levels were abundant in many tumors. These data suggest that IRS-1 contributes to the signaling pathways that lead to excessive growth stimulation in human pancreatic cancer.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1996.0500</identifier><identifier>PMID: 8607861</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Blotting, Northern ; Cell Line ; Colonic Neoplasms ; Gene Expression - drug effects ; Humans ; Insulin - biosynthesis ; Insulin - pharmacology ; Insulin Receptor Substrate Proteins ; Pancreas - metabolism ; Pancreatic Neoplasms - metabolism ; Phosphoproteins - biosynthesis ; Phosphoproteins - isolation & purification ; RNA, Messenger - analysis ; RNA, Messenger - biosynthesis ; Transcription, Genetic ; Tumor Cells, Cultured</subject><ispartof>Biochemical and biophysical research communications, 1996-03, Vol.220 (3), p.886-890</ispartof><rights>1996 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-e3fcb77a602f5a4f38a45fb30bc1beb04c20d5a490284314b3cc55152fd0eeeb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1996.0500$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8607861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bergmann, Uwe</creatorcontrib><creatorcontrib>Funatomi, Hitoshi</creatorcontrib><creatorcontrib>Kornmann, Marko</creatorcontrib><creatorcontrib>Beger, Hans G.</creatorcontrib><creatorcontrib>Korc, Murray</creatorcontrib><title>Increased Expression of Insulin Receptor Substrate-1 in Human Pancreatic Cancer</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Insulin receptor substrate-1 (IRS-1) is a multisite docking protein implicated in mitogenic signaling following activation of the insulin and insulin-like growth factor I receptors. In the present study we characterized IRS-1 expression in human pancreatic cancer. Northern blot analysis revealed high levels of IRS-1 mRNA transcripts in ASPC-1 and MIA PaCa-2 human pancreatic cancer cell lines, and lower levels in COLO-357, PANC-1, and T3M4 cells. Immunoblotting with anti-IRS-1 antibodies indicated that IRS-1 protein levels paralleled IRS-1 mRNA levels. Analysis of RNA isolated from normal and cancerous human pancreatic tissues indicated that 7 of 16 pancreatic cancer samples overexpressed IRS-1 mRNA transcripts by comparison with the normal pancreas and that insulin mRNA levels were abundant in many tumors. 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In the present study we characterized IRS-1 expression in human pancreatic cancer. Northern blot analysis revealed high levels of IRS-1 mRNA transcripts in ASPC-1 and MIA PaCa-2 human pancreatic cancer cell lines, and lower levels in COLO-357, PANC-1, and T3M4 cells. Immunoblotting with anti-IRS-1 antibodies indicated that IRS-1 protein levels paralleled IRS-1 mRNA levels. Analysis of RNA isolated from normal and cancerous human pancreatic tissues indicated that 7 of 16 pancreatic cancer samples overexpressed IRS-1 mRNA transcripts by comparison with the normal pancreas and that insulin mRNA levels were abundant in many tumors. These data suggest that IRS-1 contributes to the signaling pathways that lead to excessive growth stimulation in human pancreatic cancer.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8607861</pmid><doi>10.1006/bbrc.1996.0500</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1996-03, Vol.220 (3), p.886-890 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Blotting, Northern Cell Line Colonic Neoplasms Gene Expression - drug effects Humans Insulin - biosynthesis Insulin - pharmacology Insulin Receptor Substrate Proteins Pancreas - metabolism Pancreatic Neoplasms - metabolism Phosphoproteins - biosynthesis Phosphoproteins - isolation & purification RNA, Messenger - analysis RNA, Messenger - biosynthesis Transcription, Genetic Tumor Cells, Cultured |
| title | Increased Expression of Insulin Receptor Substrate-1 in Human Pancreatic Cancer |
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