Cloning, characterization and chromosomal location of a satellite DNA from the Pacific oyster, Crassostrea gigas
We report the cloning and characterization of a high-copy-number, tandem-repeat satellite DNA sequence from the genome of the Pacific oyster, Crassostrea gigas (Cg). The monomeric unit was found to be 166 (±2) bp in length with 79–;94% homology between monomers of the array. The sequence is A+T-rich...
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| Veröffentlicht in: | Gene 1996-02, Vol.168 (2), p.205-209 |
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| container_title | Gene |
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| creator | Clabby, Catherine Goswami, Usha Flavin, Fiona Wilkins, Noel P. Houghton, James A. Powell, Richard |
| description | We report the cloning and characterization of a high-copy-number, tandem-repeat satellite DNA sequence from the genome of the Pacific oyster,
Crassostrea gigas (Cg). The monomeric unit was found to be 166 (±2) bp in length with 79–;94% homology between monomers of the array. The sequence is A+T-rich (60%) and lacks internal repetition and substructural features. The repeat was estimated to account for 1–;4% of the
Cg genome. Fluorescence in situ hybridization (FISH) studies mapped the repeat to two distinct heterochromatic regions of two pairs of homologous chromosomes on
Cg embryonic metaphases. Also, the number of metaphase chromosomes containing this repeat varied with the ploidy of the cell. |
| doi_str_mv | 10.1016/0378-1119(95)00749-0 |
| format | Article |
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Crassostrea gigas (Cg). The monomeric unit was found to be 166 (±2) bp in length with 79–;94% homology between monomers of the array. The sequence is A+T-rich (60%) and lacks internal repetition and substructural features. The repeat was estimated to account for 1–;4% of the
Cg genome. Fluorescence in situ hybridization (FISH) studies mapped the repeat to two distinct heterochromatic regions of two pairs of homologous chromosomes on
Cg embryonic metaphases. Also, the number of metaphase chromosomes containing this repeat varied with the ploidy of the cell.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(95)00749-0</identifier><identifier>PMID: 8654945</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Base Sequence ; Chromosome Mapping ; Cloning, Molecular - methods ; Crassostrea gigas ; DNA, Satellite - genetics ; Embryo, Nonmammalian - cytology ; Embryo, Nonmammalian - physiology ; genomic content ; HaeIII and FokI repeated DNA ; heterochromatic location ; In Situ Hybridization, Fluorescence ; Metaphase ; Molecular Sequence Data ; Ostreidae - embryology ; Ostreidae - genetics ; Repetitive Sequences, Nucleic Acid ; Restriction Mapping ; sequence analysis ; Sequence Homology, Nucleic Acid ; tandem repetition</subject><ispartof>Gene, 1996-02, Vol.168 (2), p.205-209</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-7374c09bf589cc275c28f9c3fc4b5a5ff0a34175d3b2d0c480def1629c344a8c3</citedby><cites>FETCH-LOGICAL-c454t-7374c09bf589cc275c28f9c3fc4b5a5ff0a34175d3b2d0c480def1629c344a8c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(95)00749-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8654945$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clabby, Catherine</creatorcontrib><creatorcontrib>Goswami, Usha</creatorcontrib><creatorcontrib>Flavin, Fiona</creatorcontrib><creatorcontrib>Wilkins, Noel P.</creatorcontrib><creatorcontrib>Houghton, James A.</creatorcontrib><creatorcontrib>Powell, Richard</creatorcontrib><title>Cloning, characterization and chromosomal location of a satellite DNA from the Pacific oyster, Crassostrea gigas</title><title>Gene</title><addtitle>Gene</addtitle><description>We report the cloning and characterization of a high-copy-number, tandem-repeat satellite DNA sequence from the genome of the Pacific oyster,
Crassostrea gigas (Cg). The monomeric unit was found to be 166 (±2) bp in length with 79–;94% homology between monomers of the array. The sequence is A+T-rich (60%) and lacks internal repetition and substructural features. The repeat was estimated to account for 1–;4% of the
Cg genome. Fluorescence in situ hybridization (FISH) studies mapped the repeat to two distinct heterochromatic regions of two pairs of homologous chromosomes on
Cg embryonic metaphases. Also, the number of metaphase chromosomes containing this repeat varied with the ploidy of the cell.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Chromosome Mapping</subject><subject>Cloning, Molecular - methods</subject><subject>Crassostrea gigas</subject><subject>DNA, Satellite - genetics</subject><subject>Embryo, Nonmammalian - cytology</subject><subject>Embryo, Nonmammalian - physiology</subject><subject>genomic content</subject><subject>HaeIII and FokI repeated DNA</subject><subject>heterochromatic location</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Metaphase</subject><subject>Molecular Sequence Data</subject><subject>Ostreidae - embryology</subject><subject>Ostreidae - genetics</subject><subject>Repetitive Sequences, Nucleic Acid</subject><subject>Restriction Mapping</subject><subject>sequence analysis</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>tandem repetition</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9PHCEUxUlTo-vab1ATnhpNnAozsAwvJmbrv8S0PthncvcOrDQzwxbYJvrpy7obH5UXyD0_DuQcQr5y9p0zPjtnjWorzrk-0fKUMSV0xT6RCW9VObCm_Uwmb8gBOUzpDytLynqf7LczKbSQE7Ka92H04_KM4hNEwGyjf4Hsw0hh7MowhiGkMEBP-4BbITgKNEG2fe-zpT9-XlJXMJqfLH0A9M4jDc-pWJ3ReYSUQsrRAl36JaQjsuegT_bLbp-S39dXj_Pb6v7Xzd388r5CIUWuVKMEMr1wstWItZJYt05j41AsJEjnGDSCK9k1i7pjKFrWWcdndUGEgBabKfm29V3F8HdtUzaDT1i-DKMN62SU0lqJGf8Q5IpxKYQuoNiCGENK0Tqzin6A-Gw4M5tGzCZus4nbaGleGymjKTne-a8Xg-3eLu0qKPrFVrcljX_eRpPQ2xFt56PFbLrg33_gP5Ckm0Q</recordid><startdate>19960212</startdate><enddate>19960212</enddate><creator>Clabby, Catherine</creator><creator>Goswami, Usha</creator><creator>Flavin, Fiona</creator><creator>Wilkins, Noel P.</creator><creator>Houghton, James A.</creator><creator>Powell, Richard</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19960212</creationdate><title>Cloning, characterization and chromosomal location of a satellite DNA from the Pacific oyster, Crassostrea gigas</title><author>Clabby, Catherine ; 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Crassostrea gigas (Cg). The monomeric unit was found to be 166 (±2) bp in length with 79–;94% homology between monomers of the array. The sequence is A+T-rich (60%) and lacks internal repetition and substructural features. The repeat was estimated to account for 1–;4% of the
Cg genome. Fluorescence in situ hybridization (FISH) studies mapped the repeat to two distinct heterochromatic regions of two pairs of homologous chromosomes on
Cg embryonic metaphases. Also, the number of metaphase chromosomes containing this repeat varied with the ploidy of the cell.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8654945</pmid><doi>10.1016/0378-1119(95)00749-0</doi><tpages>5</tpages></addata></record> |
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| ispartof | Gene, 1996-02, Vol.168 (2), p.205-209 |
| issn | 0378-1119 1879-0038 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Base Sequence Chromosome Mapping Cloning, Molecular - methods Crassostrea gigas DNA, Satellite - genetics Embryo, Nonmammalian - cytology Embryo, Nonmammalian - physiology genomic content HaeIII and FokI repeated DNA heterochromatic location In Situ Hybridization, Fluorescence Metaphase Molecular Sequence Data Ostreidae - embryology Ostreidae - genetics Repetitive Sequences, Nucleic Acid Restriction Mapping sequence analysis Sequence Homology, Nucleic Acid tandem repetition |
| title | Cloning, characterization and chromosomal location of a satellite DNA from the Pacific oyster, Crassostrea gigas |
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