Transgenic rice established to express corn cystatin exhibits strong inhibitory activity against insect gut proteinases

Corn cystatin (CC), a phytocystatin, shows a wide inhibitory spectrum against various cysteine proteinases. We produced transgenic rice plants by introducing CC cDNA under CaMV 35S promoter as a first step to obtain a rice plant with insecticidal activity. This attempt was based on the observation t...

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Veröffentlicht in:	Plant molecular biology 1996, Vol.30 (1), p.149-157
Hauptverfasser:	Irie, K, Hosoyama, H, Takeuchi, T, Iwabuchi, K, Watanabe, H, Abe, M, Abe, K, Arai, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence cathepsins Coleoptera Coleoptera - enzymology complementary DNA Cystatins - biosynthesis Cystatins - genetics Cysteine Proteinase Inhibitors - biosynthesis Cysteine Proteinase Inhibitors - genetics Digestive System - enzymology Dose-Response Relationship, Drug enzyme activity gene expression gene transfer Genes, Plant immunohistochemistry inhibition leaves messenger RNA midgut midgut proteinases Molecular Sequence Data Oryza - genetics Oryza sativa papain plant proteins Plants, Genetically Modified proteinase inhibitors RNA, Messenger - biosynthesis RNA, Plant - biosynthesis Sitophilus Sitophilus zeamais Tissue Distribution transgenic plants Zea mays Zea mays - enzymology Zea mays - genetics
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