Endothelin Stimulates Protein Phosphorylation in Blood–Brain Barrier
The vasoactive neuropeptide endothelin stimulated phosphorylation of specific proteins in rat brain microvessels. Major effects were observed with endothelin-1. These effects were dose- and time-dependent. Among substrates for kinase actions, the 17.5 KDa and 80 KDa proteins were mainly affected by...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1996-02, Vol.219 (2), p.366-369 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 369 |
|---|---|
| container_issue | 2 |
| container_start_page | 366 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 219 |
| creator | Catalán, R.E. Martı́nez, A.M. Aragonés, M.D. Hernández, F. Dı́az, G. |
| description | The vasoactive neuropeptide endothelin stimulated phosphorylation of specific proteins in rat brain microvessels. Major effects were observed with endothelin-1. These effects were dose- and time-dependent. Among substrates for kinase actions, the 17.5 KDa and 80 KDa proteins were mainly affected by endothelin-1. It was demonstrated that the 80 KDa protein affected corresponds to MARCKS (myristoylated alanine-rich C kinase substrate), a specific substrate for protein kinase C action. |
| doi_str_mv | 10.1006/bbrc.1996.0239 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77991496</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X96902399</els_id><sourcerecordid>77991496</sourcerecordid><originalsourceid>FETCH-LOGICAL-c341t-2fb6a79b4a9973e4f3b0c92f365cdcd606ab8031c3b27e79ced0a3725109dce13</originalsourceid><addsrcrecordid>eNp1kEFLAzEQhYMotVav3oSevO062WyzzdFKq0LBggreQjaZpZHtpia7Qm_-B_-hv8QsLd48DXnz5pH3EXJJIaUA_KYsvU6pEDyFjIkjMqQgIMko5MdkCNGRZIK-nZKzEN4BKM25GJDBlEMuBBuSxbwxrl1jbZvxc2s3Xa1aDOOVdy1GabV2Ybt2fhdl65pxlGa1c-bn63vmVf9S3lv05-SkUnXAi8MckdfF_OXuIVk-3T_e3S4TzXLaJllVclWIMldCFAzzipWgRVYxPtFGGw5clVNgVLMyK7AQGg0oVmSTWMpopGxErve5W-8-Ogyt3Nigsa5Vg64LsiiEoLng0Zjujdq7EDxWcuvtRvmdpCB7cLIHJ3twsgcXD64OyV25QfNnP5CK--l-j7HeZ6wsg7bYxB9aj7qVxtn_on8BN4F-Rw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77991496</pqid></control><display><type>article</type><title>Endothelin Stimulates Protein Phosphorylation in Blood–Brain Barrier</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Catalán, R.E. ; Martı́nez, A.M. ; Aragonés, M.D. ; Hernández, F. ; Dı́az, G.</creator><creatorcontrib>Catalán, R.E. ; Martı́nez, A.M. ; Aragonés, M.D. ; Hernández, F. ; Dı́az, G.</creatorcontrib><description>The vasoactive neuropeptide endothelin stimulated phosphorylation of specific proteins in rat brain microvessels. Major effects were observed with endothelin-1. These effects were dose- and time-dependent. Among substrates for kinase actions, the 17.5 KDa and 80 KDa proteins were mainly affected by endothelin-1. It was demonstrated that the 80 KDa protein affected corresponds to MARCKS (myristoylated alanine-rich C kinase substrate), a specific substrate for protein kinase C action.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1996.0239</identifier><identifier>PMID: 8604993</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Blood-Brain Barrier ; Brain - blood supply ; Cerebrovascular Circulation ; Electrophoresis, Polyacrylamide Gel ; Endothelins - pharmacology ; Intracellular Signaling Peptides and Proteins ; Male ; Membrane Proteins ; Microcirculation - metabolism ; Molecular Weight ; Myristoylated Alanine-Rich C Kinase Substrate ; Phosphates - metabolism ; Phosphoproteins - isolation & purification ; Phosphoproteins - metabolism ; Phosphorus Radioisotopes ; Phosphorylation ; Protein Kinase C - metabolism ; Proteins - isolation & purification ; Proteins - metabolism ; Rats ; Rats, Wistar ; Substrate Specificity</subject><ispartof>Biochemical and biophysical research communications, 1996-02, Vol.219 (2), p.366-369</ispartof><rights>1996 Academic Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-2fb6a79b4a9973e4f3b0c92f365cdcd606ab8031c3b27e79ced0a3725109dce13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1996.0239$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8604993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Catalán, R.E.</creatorcontrib><creatorcontrib>Martı́nez, A.M.</creatorcontrib><creatorcontrib>Aragonés, M.D.</creatorcontrib><creatorcontrib>Hernández, F.</creatorcontrib><creatorcontrib>Dı́az, G.</creatorcontrib><title>Endothelin Stimulates Protein Phosphorylation in Blood–Brain Barrier</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>The vasoactive neuropeptide endothelin stimulated phosphorylation of specific proteins in rat brain microvessels. Major effects were observed with endothelin-1. These effects were dose- and time-dependent. Among substrates for kinase actions, the 17.5 KDa and 80 KDa proteins were mainly affected by endothelin-1. It was demonstrated that the 80 KDa protein affected corresponds to MARCKS (myristoylated alanine-rich C kinase substrate), a specific substrate for protein kinase C action.</description><subject>Animals</subject><subject>Blood-Brain Barrier</subject><subject>Brain - blood supply</subject><subject>Cerebrovascular Circulation</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Endothelins - pharmacology</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>Male</subject><subject>Membrane Proteins</subject><subject>Microcirculation - metabolism</subject><subject>Molecular Weight</subject><subject>Myristoylated Alanine-Rich C Kinase Substrate</subject><subject>Phosphates - metabolism</subject><subject>Phosphoproteins - isolation & purification</subject><subject>Phosphoproteins - metabolism</subject><subject>Phosphorus Radioisotopes</subject><subject>Phosphorylation</subject><subject>Protein Kinase C - metabolism</subject><subject>Proteins - isolation & purification</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Substrate Specificity</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEFLAzEQhYMotVav3oSevO062WyzzdFKq0LBggreQjaZpZHtpia7Qm_-B_-hv8QsLd48DXnz5pH3EXJJIaUA_KYsvU6pEDyFjIkjMqQgIMko5MdkCNGRZIK-nZKzEN4BKM25GJDBlEMuBBuSxbwxrl1jbZvxc2s3Xa1aDOOVdy1GabV2Ybt2fhdl65pxlGa1c-bn63vmVf9S3lv05-SkUnXAi8MckdfF_OXuIVk-3T_e3S4TzXLaJllVclWIMldCFAzzipWgRVYxPtFGGw5clVNgVLMyK7AQGg0oVmSTWMpopGxErve5W-8-Ogyt3Nigsa5Vg64LsiiEoLng0Zjujdq7EDxWcuvtRvmdpCB7cLIHJ3twsgcXD64OyV25QfNnP5CK--l-j7HeZ6wsg7bYxB9aj7qVxtn_on8BN4F-Rw</recordid><startdate>19960215</startdate><enddate>19960215</enddate><creator>Catalán, R.E.</creator><creator>Martı́nez, A.M.</creator><creator>Aragonés, M.D.</creator><creator>Hernández, F.</creator><creator>Dı́az, G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960215</creationdate><title>Endothelin Stimulates Protein Phosphorylation in Blood–Brain Barrier</title><author>Catalán, R.E. ; Martı́nez, A.M. ; Aragonés, M.D. ; Hernández, F. ; Dı́az, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-2fb6a79b4a9973e4f3b0c92f365cdcd606ab8031c3b27e79ced0a3725109dce13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Blood-Brain Barrier</topic><topic>Brain - blood supply</topic><topic>Cerebrovascular Circulation</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Endothelins - pharmacology</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>Male</topic><topic>Membrane Proteins</topic><topic>Microcirculation - metabolism</topic><topic>Molecular Weight</topic><topic>Myristoylated Alanine-Rich C Kinase Substrate</topic><topic>Phosphates - metabolism</topic><topic>Phosphoproteins - isolation & purification</topic><topic>Phosphoproteins - metabolism</topic><topic>Phosphorus Radioisotopes</topic><topic>Phosphorylation</topic><topic>Protein Kinase C - metabolism</topic><topic>Proteins - isolation & purification</topic><topic>Proteins - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Substrate Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Catalán, R.E.</creatorcontrib><creatorcontrib>Martı́nez, A.M.</creatorcontrib><creatorcontrib>Aragonés, M.D.</creatorcontrib><creatorcontrib>Hernández, F.</creatorcontrib><creatorcontrib>Dı́az, G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Catalán, R.E.</au><au>Martı́nez, A.M.</au><au>Aragonés, M.D.</au><au>Hernández, F.</au><au>Dı́az, G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelin Stimulates Protein Phosphorylation in Blood–Brain Barrier</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1996-02-15</date><risdate>1996</risdate><volume>219</volume><issue>2</issue><spage>366</spage><epage>369</epage><pages>366-369</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>The vasoactive neuropeptide endothelin stimulated phosphorylation of specific proteins in rat brain microvessels. Major effects were observed with endothelin-1. These effects were dose- and time-dependent. Among substrates for kinase actions, the 17.5 KDa and 80 KDa proteins were mainly affected by endothelin-1. It was demonstrated that the 80 KDa protein affected corresponds to MARCKS (myristoylated alanine-rich C kinase substrate), a specific substrate for protein kinase C action.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8604993</pmid><doi>10.1006/bbrc.1996.0239</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1996-02, Vol.219 (2), p.366-369 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77991496 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Blood-Brain Barrier Brain - blood supply Cerebrovascular Circulation Electrophoresis, Polyacrylamide Gel Endothelins - pharmacology Intracellular Signaling Peptides and Proteins Male Membrane Proteins Microcirculation - metabolism Molecular Weight Myristoylated Alanine-Rich C Kinase Substrate Phosphates - metabolism Phosphoproteins - isolation & purification Phosphoproteins - metabolism Phosphorus Radioisotopes Phosphorylation Protein Kinase C - metabolism Proteins - isolation & purification Proteins - metabolism Rats Rats, Wistar Substrate Specificity |
| title | Endothelin Stimulates Protein Phosphorylation in Blood–Brain Barrier |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T04%3A25%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Endothelin%20Stimulates%20Protein%20Phosphorylation%20in%20Blood%E2%80%93Brain%20Barrier&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Catal%C3%A1n,%20R.E.&rft.date=1996-02-15&rft.volume=219&rft.issue=2&rft.spage=366&rft.epage=369&rft.pages=366-369&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1006/bbrc.1996.0239&rft_dat=%3Cproquest_cross%3E77991496%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77991496&rft_id=info:pmid/8604993&rft_els_id=S0006291X96902399&rfr_iscdi=true |