Identification of a developmentally regulated gene, esr16, in the tracheal epithelium of Manduca sexta, with homology to a protein from human epididymis
Events related to both ecdysis and metamorphosis require the expression of a new set of genes, the majority of which are regulated by the changes in ecdysteroid levels. We have initiated studies to identify genes whose expression is up-regulated between 24 and 4 h before pupal ecdysis in Manduca sex...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 1996, Vol.26 (1), p.7-11 |
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| container_title | Insect biochemistry and molecular biology |
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| creator | Mészáros, Melinda Morton, David.B. |
| description | Events related to both ecdysis and metamorphosis require the expression of a new set of genes, the majority of which are regulated by the changes in ecdysteroid levels. We have initiated studies to identify genes whose expression is up-regulated between 24 and 4 h before pupal ecdysis in
Manduca sexta. In this paper we report the partial characterization of one such gene,
esrl6. The transcript of
esr16 is detected by Northern blot analysis in nervous tissue, muscle and trachea isolated from animals 4 h before, but not 24 h before pupal ecdysis.
In situ hybridization showed that the transcript was expressed in epithelial cells of the large tracheae surrounding the nervous system and muscle. Sequence analysis suggested that the gene encoded a secreted protein with 35% identity to HE1, a human epididymal-specific gene. |
| doi_str_mv | 10.1016/0965-1748(95)00077-1 |
| format | Article |
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Manduca sexta. In this paper we report the partial characterization of one such gene,
esrl6. The transcript of
esr16 is detected by Northern blot analysis in nervous tissue, muscle and trachea isolated from animals 4 h before, but not 24 h before pupal ecdysis.
In situ hybridization showed that the transcript was expressed in epithelial cells of the large tracheae surrounding the nervous system and muscle. Sequence analysis suggested that the gene encoded a secreted protein with 35% identity to HE1, a human epididymal-specific gene.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/0965-1748(95)00077-1</identifier><identifier>PMID: 8673080</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Carrier Proteins ; Ecdysone ; Ecdysteroid ; Ecdysterone - metabolism ; Epididymis ; Epididymis - chemistry ; Gene ; Gene Expression Regulation, Developmental ; Genes, Insect ; Glycoproteins ; Humans ; Insect Proteins ; Lepidoptera ; Male ; Manduca ; Manduca - genetics ; Manduca sexta ; Molecular Sequence Data ; Proteins - chemistry ; Proteins - genetics ; Proteins - metabolism ; regulation ; Sphingidae ; Tracheae</subject><ispartof>Insect biochemistry and molecular biology, 1996, Vol.26 (1), p.7-11</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c388t-de3e21f5247b2e95379a1c9691d79b774cc2efd2d7dfe3fc03c888e8faec3a4a3</citedby><cites>FETCH-LOGICAL-c388t-de3e21f5247b2e95379a1c9691d79b774cc2efd2d7dfe3fc03c888e8faec3a4a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0965174895000771$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8673080$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mészáros, Melinda</creatorcontrib><creatorcontrib>Morton, David.B.</creatorcontrib><title>Identification of a developmentally regulated gene, esr16, in the tracheal epithelium of Manduca sexta, with homology to a protein from human epididymis</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>Events related to both ecdysis and metamorphosis require the expression of a new set of genes, the majority of which are regulated by the changes in ecdysteroid levels. We have initiated studies to identify genes whose expression is up-regulated between 24 and 4 h before pupal ecdysis in
Manduca sexta. In this paper we report the partial characterization of one such gene,
esrl6. The transcript of
esr16 is detected by Northern blot analysis in nervous tissue, muscle and trachea isolated from animals 4 h before, but not 24 h before pupal ecdysis.
In situ hybridization showed that the transcript was expressed in epithelial cells of the large tracheae surrounding the nervous system and muscle. Sequence analysis suggested that the gene encoded a secreted protein with 35% identity to HE1, a human epididymal-specific gene.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Carrier Proteins</subject><subject>Ecdysone</subject><subject>Ecdysteroid</subject><subject>Ecdysterone - metabolism</subject><subject>Epididymis</subject><subject>Epididymis - chemistry</subject><subject>Gene</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genes, Insect</subject><subject>Glycoproteins</subject><subject>Humans</subject><subject>Insect Proteins</subject><subject>Lepidoptera</subject><subject>Male</subject><subject>Manduca</subject><subject>Manduca - genetics</subject><subject>Manduca sexta</subject><subject>Molecular Sequence Data</subject><subject>Proteins - chemistry</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>regulation</subject><subject>Sphingidae</subject><subject>Tracheae</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9vFCEUxYmxqWv1G2jCk9FkR2GYGeCliWn806TGF30mLFx2McywAlPdb9KPW8bd9NE-3dycc3-HcBB6Rcl7SujwgcihbyjvxFvZvyOEcN7QJ2hFBZcNaTvyFK0eLM_Q85x_VVPX9fwcnYuBMyLICt1dW5iKd97o4uOEo8MaW7iFEPdjVXQIB5xgOwddwOItTLDGkBMd1thPuOwAl6TNDnTAsPd1D34eF8w3PdnZaJzhb9Fr_KdqeBfHGOL2gEusMfsUC1SIS3HEu3nU04Kw3h5Gn1-gM6dDhpeneYF-fv704-prc_P9y_XVx5vGMCFKY4FBS13fdnzTguwZl5oaOUhqudxw3hnTgrOt5dYBc4YwI4QA4TQYpjvNLtCbI7e-5vcMuagabiAEPUGcs-JcSlqpjxopJ4z33WLsjkaTYs4JnNonP-p0UJSopTm11KKWWpTs1b_mFK1nr0_8eTOCfTg6VVX1y6MO9TduPSSVjYfJgPUJTFE2-v8H3APgJ6sE</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Mészáros, Melinda</creator><creator>Morton, David.B.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>Identification of a developmentally regulated gene, esr16, in the tracheal epithelium of Manduca sexta, with homology to a protein from human epididymis</title><author>Mészáros, Melinda ; Morton, David.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-de3e21f5247b2e95379a1c9691d79b774cc2efd2d7dfe3fc03c888e8faec3a4a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Carrier Proteins</topic><topic>Ecdysone</topic><topic>Ecdysteroid</topic><topic>Ecdysterone - metabolism</topic><topic>Epididymis</topic><topic>Epididymis - chemistry</topic><topic>Gene</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genes, Insect</topic><topic>Glycoproteins</topic><topic>Humans</topic><topic>Insect Proteins</topic><topic>Lepidoptera</topic><topic>Male</topic><topic>Manduca</topic><topic>Manduca - genetics</topic><topic>Manduca sexta</topic><topic>Molecular Sequence Data</topic><topic>Proteins - chemistry</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>regulation</topic><topic>Sphingidae</topic><topic>Tracheae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mészáros, Melinda</creatorcontrib><creatorcontrib>Morton, David.B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mészáros, Melinda</au><au>Morton, David.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a developmentally regulated gene, esr16, in the tracheal epithelium of Manduca sexta, with homology to a protein from human epididymis</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>1996</date><risdate>1996</risdate><volume>26</volume><issue>1</issue><spage>7</spage><epage>11</epage><pages>7-11</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>Events related to both ecdysis and metamorphosis require the expression of a new set of genes, the majority of which are regulated by the changes in ecdysteroid levels. We have initiated studies to identify genes whose expression is up-regulated between 24 and 4 h before pupal ecdysis in
Manduca sexta. In this paper we report the partial characterization of one such gene,
esrl6. The transcript of
esr16 is detected by Northern blot analysis in nervous tissue, muscle and trachea isolated from animals 4 h before, but not 24 h before pupal ecdysis.
In situ hybridization showed that the transcript was expressed in epithelial cells of the large tracheae surrounding the nervous system and muscle. Sequence analysis suggested that the gene encoded a secreted protein with 35% identity to HE1, a human epididymal-specific gene.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>8673080</pmid><doi>10.1016/0965-1748(95)00077-1</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0965-1748 |
| ispartof | Insect biochemistry and molecular biology, 1996, Vol.26 (1), p.7-11 |
| issn | 0965-1748 1879-0240 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Amino Acid Sequence Animals Base Sequence Carrier Proteins Ecdysone Ecdysteroid Ecdysterone - metabolism Epididymis Epididymis - chemistry Gene Gene Expression Regulation, Developmental Genes, Insect Glycoproteins Humans Insect Proteins Lepidoptera Male Manduca Manduca - genetics Manduca sexta Molecular Sequence Data Proteins - chemistry Proteins - genetics Proteins - metabolism regulation Sphingidae Tracheae |
| title | Identification of a developmentally regulated gene, esr16, in the tracheal epithelium of Manduca sexta, with homology to a protein from human epididymis |
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