Immunocytochemical detection of Entamoeba histolytica
Human anti- Entamoeba histolytica immunoglobulin was used to detect Entamoeba histolytica in 74 positive samples from several different sources, using an indirect immunoperoxidase method. In 73 samples, the protozoan was easily identified. Trophozoites and cysts of all cultured Entamoeba strains exa...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 1987-01, Vol.81 (4), p.624-626 |
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| container_title | Transactions of the Royal Society of Tropical Medicine and Hygiene |
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| creator | de Súarez, Eva Pérez Pérez-Schael, Irene Perozo-Ruggeri, Gladys de Dávila, Delia Römer, Hans Tapia, Felix J. |
| description | Human anti-
Entamoeba histolytica immunoglobulin was used to detect
Entamoeba histolytica in 74 positive samples from several different sources, using an indirect immunoperoxidase method. In 73 samples, the protozoan was easily identified. Trophozoites and cysts of all cultured
Entamoeba strains examined were strongly stained, and as few as 3 trophozoites per microscope slide could be detected. In addition, 51 negative control samples were also tested and non-specific reactions were not observed. These preliminary results show that this method is both sensitive and specific, and can easily detect trophozoites and cysts of different
E. histolytica strains. |
| doi_str_mv | 10.1016/0035-9203(87)90435-4 |
| format | Article |
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Entamoeba histolytica immunoglobulin was used to detect
Entamoeba histolytica in 74 positive samples from several different sources, using an indirect immunoperoxidase method. In 73 samples, the protozoan was easily identified. Trophozoites and cysts of all cultured
Entamoeba strains examined were strongly stained, and as few as 3 trophozoites per microscope slide could be detected. In addition, 51 negative control samples were also tested and non-specific reactions were not observed. These preliminary results show that this method is both sensitive and specific, and can easily detect trophozoites and cysts of different
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Entamoeba histolytica immunoglobulin was used to detect
Entamoeba histolytica in 74 positive samples from several different sources, using an indirect immunoperoxidase method. In 73 samples, the protozoan was easily identified. Trophozoites and cysts of all cultured
Entamoeba strains examined were strongly stained, and as few as 3 trophozoites per microscope slide could be detected. In addition, 51 negative control samples were also tested and non-specific reactions were not observed. These preliminary results show that this method is both sensitive and specific, and can easily detect trophozoites and cysts of different
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Psychology</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Infectious diseases</subject><subject>Liver Abscess, Amebic - diagnosis</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>Protozoa</subject><subject>Protozoal diseases</subject><subject>Tropical medicine</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1LYzEUhoM4aO34DxS6ENHFdfJ1mmQjSHFqQXQWI4ibkCa5GL33RpNbsf_edFq6dFbh8D7nJTwHoSOCLwgm418YM6gUxexMinOFeZn4DhoQKWTFALNdNNgi--gg5xeMKRBQe2iPSgVA2ADBrG0XXbTLPtpn3wZrmpHzvbd9iN0o1qPrrjdt9HMzeg65j82yL8xP9KM2TfaHm3eIHn5f_53cVLf309nk6rayHKCveC0EWMKFY1xyYpziuKaMWDXngnhmQdWGMwrUMUrHc6iN45aCsM4QgxkbotN171uK7wufe92GbH3TmM7HRdZCKKlK_39BwqVQRMoC8jVoU8w5-Vq_pdCatNQE65VWvXKmV860FPqfVs3L2vGmfzFvvdsubTyW_GSTm1wU1sl0NuQtJss9hIKCVWusqPSf29ikVz0WTIC-eXzSoPCfyR2f6mnhL9e8L5I_gk862-A7611I5UTaxfD9v78AVSqhJQ</recordid><startdate>19870101</startdate><enddate>19870101</enddate><creator>de Súarez, Eva Pérez</creator><creator>Pérez-Schael, Irene</creator><creator>Perozo-Ruggeri, Gladys</creator><creator>de Dávila, Delia</creator><creator>Römer, Hans</creator><creator>Tapia, Felix J.</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><general>Elsevier</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>19870101</creationdate><title>Immunocytochemical detection of Entamoeba histolytica</title><author>de Súarez, Eva Pérez ; Pérez-Schael, Irene ; Perozo-Ruggeri, Gladys ; de Dávila, Delia ; Römer, Hans ; Tapia, Felix J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-4f775c147d34841ad940f231c9b471e3c59fa43252d3226b5fad4c257cda1a033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Amebiasis - diagnosis</topic><topic>Amibiasis</topic><topic>Animals</topic><topic>Antigens, Protozoan - analysis</topic><topic>Biochemistry. 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Entamoeba histolytica immunoglobulin was used to detect
Entamoeba histolytica in 74 positive samples from several different sources, using an indirect immunoperoxidase method. In 73 samples, the protozoan was easily identified. Trophozoites and cysts of all cultured
Entamoeba strains examined were strongly stained, and as few as 3 trophozoites per microscope slide could be detected. In addition, 51 negative control samples were also tested and non-specific reactions were not observed. These preliminary results show that this method is both sensitive and specific, and can easily detect trophozoites and cysts of different
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| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 1987-01, Vol.81 (4), p.624-626 |
| issn | 0035-9203 1878-3503 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amebiasis - diagnosis Amibiasis Animals Antigens, Protozoan - analysis Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Entamoeba histolytica Entamoeba histolytica - immunology Entamoebiasis - diagnosis Feces - parasitology Fundamental and applied biological sciences. Psychology Human protozoal diseases Humans Immunoenzyme Techniques Infectious diseases Liver Abscess, Amebic - diagnosis Medical sciences Parasitic diseases Protozoa Protozoal diseases Tropical medicine |
| title | Immunocytochemical detection of Entamoeba histolytica |
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