The Guanylyl Cyclase Core of an Atrial Natriuretic Peptide Receptor: Enzymatic Properties and Basis for Cooperativity

The enzymatic properties of a cloned atrial natriuretic peptide receptor are described. The renatured catalytic core had maximal activity with Mn2+, and all nucleoside triphosphates inhibited the enzyme competitively. The catalytic specificity of the enzyme was tested directly. The cyclase reaction...

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Veröffentlicht in:	Biochemical and biophysical research communications 1996-01, Vol.218 (3), p.670-673
Hauptverfasser:	Thorpe, David S., Niu, Shi, Morkin, Eugene
Format:	Artikel
Sprache:	eng
Schlagworte:	Allosteric Regulation Cations, Divalent Deoxyribonucleotides - chemistry Deoxyribonucleotides - metabolism Guanosine Triphosphate - metabolism Guanylate Cyclase - antagonists & inhibitors Guanylate Cyclase - chemistry Guanylate Cyclase - metabolism Kinetics Nucleotides - chemistry Nucleotides - metabolism Receptors, Atrial Natriuretic Factor - chemistry Recombinant Proteins Signal Transduction Substrate Specificity
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container_end_page	673
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container_title	Biochemical and biophysical research communications
container_volume	218
creator	Thorpe, David S. Niu, Shi Morkin, Eugene
description	The enzymatic properties of a cloned atrial natriuretic peptide receptor are described. The renatured catalytic core had maximal activity with Mn2+, and all nucleoside triphosphates inhibited the enzyme competitively. The catalytic specificity of the enzyme was tested directly. The cyclase reaction was specific for guanine, producing cGMP and cyclic deoxyGMP. Surprisingly, deoxyguanylyl cyclase kinetics were classical, unlike the positive cooperativity seen for guanylyl cyclase activity, suggesting that the 2′ hydroxyl group of GTP is necessary for the allosteric mechanism.
doi_str_mv	10.1006/bbrc.1996.0120
format	Article
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subjects	Allosteric Regulation Cations, Divalent Deoxyribonucleotides - chemistry Deoxyribonucleotides - metabolism Guanosine Triphosphate - metabolism Guanylate Cyclase - antagonists & inhibitors Guanylate Cyclase - chemistry Guanylate Cyclase - metabolism Kinetics Nucleotides - chemistry Nucleotides - metabolism Receptors, Atrial Natriuretic Factor - chemistry Recombinant Proteins Signal Transduction Substrate Specificity
title	The Guanylyl Cyclase Core of an Atrial Natriuretic Peptide Receptor: Enzymatic Properties and Basis for Cooperativity
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