Cysteine requirements of naturally occurring cysteine auxotrophs of Escherichia coli
The requirements for cysteine of naturally occurring cysteine auxotrophs of Escherichia coli were determined in a defined liquid medium. Maximal growth was obtained in the presence of cysteine concentrations between 20 and 250mg/l. At concentrations below 20mg/l growth of the auxotrophs, but not the...
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| Veröffentlicht in: | Pathology 1987, Vol.19 (4), p.361-363 |
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| container_title | Pathology |
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| creator | McIver, C.J. Tapsall, J.W. |
| description | The requirements for cysteine of naturally occurring cysteine auxotrophs of Escherichia coli were determined in a defined liquid medium. Maximal growth was obtained in the presence of cysteine concentrations between 20 and 250mg/l. At concentrations below 20mg/l growth of the auxotrophs, but not the prototrophic control, was suboptimal in this system. In the presence of cysteine concentrations in excess of 250mg/l, growth of both auxotrophic and prototrophic E. coli was inhibited with lower growth yields, a decreased specific growth rate and an extended lag phase being observed. These effects were minimised in the presence of 2mM L-leucine, L-isoleucine and L-valine. |
| doi_str_mv | 10.3109/00313028709103884 |
| format | Article |
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Maximal growth was obtained in the presence of cysteine concentrations between 20 and 250mg/l. At concentrations below 20mg/l growth of the auxotrophs, but not the prototrophic control, was suboptimal in this system. In the presence of cysteine concentrations in excess of 250mg/l, growth of both auxotrophic and prototrophic E. coli was inhibited with lower growth yields, a decreased specific growth rate and an extended lag phase being observed. These effects were minimised in the presence of 2mM L-leucine, L-isoleucine and L-valine.</description><identifier>ISSN: 0031-3025</identifier><identifier>EISSN: 1465-3931</identifier><identifier>DOI: 10.3109/00313028709103884</identifier><identifier>PMID: 3328142</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Cysteine ; Cysteine - metabolism ; cystine ; E. coli ; Escherichia coli - growth & development ; Escherichia coli - metabolism ; Mutation ; nutritional requirement</subject><ispartof>Pathology, 1987, Vol.19 (4), p.361-363</ispartof><rights>1987 Royal College of Pathologists of Australasia</rights><rights>1987 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1987</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-9df0b337adec63dccc9a1277d3c11dc844871ff6c757c70c4d4edbba43b3ea523</citedby><cites>FETCH-LOGICAL-c400t-9df0b337adec63dccc9a1277d3c11dc844871ff6c757c70c4d4edbba43b3ea523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/00313028709103884$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/00313028709103884$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,61221,61402</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3328142$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McIver, C.J.</creatorcontrib><creatorcontrib>Tapsall, J.W.</creatorcontrib><title>Cysteine requirements of naturally occurring cysteine auxotrophs of Escherichia coli</title><title>Pathology</title><addtitle>Pathology</addtitle><description>The requirements for cysteine of naturally occurring cysteine auxotrophs of Escherichia coli were determined in a defined liquid medium. Maximal growth was obtained in the presence of cysteine concentrations between 20 and 250mg/l. At concentrations below 20mg/l growth of the auxotrophs, but not the prototrophic control, was suboptimal in this system. In the presence of cysteine concentrations in excess of 250mg/l, growth of both auxotrophic and prototrophic E. coli was inhibited with lower growth yields, a decreased specific growth rate and an extended lag phase being observed. These effects were minimised in the presence of 2mM L-leucine, L-isoleucine and L-valine.</description><subject>Cysteine</subject><subject>Cysteine - metabolism</subject><subject>cystine</subject><subject>E. coli</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli - metabolism</subject><subject>Mutation</subject><subject>nutritional requirement</subject><issn>0031-3025</issn><issn>1465-3931</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1LxDAURYMoOo7-ABdCV-6qSZM2Ka5kGD9AcKPrkL682kjbjEkrzr-344xuBFdZ3HMv5DxCzhi95IyWV5RyxmmmJC0Z5UqJPTJjoshTXnK2T2abPJ2A_Igcx_hGKRVKqUNyyHmmmMhm5HmxjgO6HpOA76ML2GE_xMTXSW-GMZi2XSceYAzB9a8J_MBm_PRD8KvmG11GaDA4aJxJwLfuhBzUpo14unvn5OV2-by4Tx-f7h4WN48pCEqHtLQ1rTiXxiIU3AJAaVgmpeXAmAUlhJKsrguQuQRJQViBtqqM4BVHk2d8Ti62u6vg30eMg-5cBGxb06Mfo5ayVHlRiAlkWxCCjzFgrVfBdSasNaN6Y1L_MTl1znfjY9Wh_W3s1E359TZ3fe1DZxo07dCACajf_Bj66eP_ru_aOPn5cBh0BIc9oJ1uAIO23v3T_gLxtZUm</recordid><startdate>1987</startdate><enddate>1987</enddate><creator>McIver, C.J.</creator><creator>Tapsall, J.W.</creator><general>Elsevier B.V</general><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1987</creationdate><title>Cysteine requirements of naturally occurring cysteine auxotrophs of Escherichia coli</title><author>McIver, C.J. ; Tapsall, J.W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-9df0b337adec63dccc9a1277d3c11dc844871ff6c757c70c4d4edbba43b3ea523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Cysteine</topic><topic>Cysteine - metabolism</topic><topic>cystine</topic><topic>E. coli</topic><topic>Escherichia coli - growth & development</topic><topic>Escherichia coli - metabolism</topic><topic>Mutation</topic><topic>nutritional requirement</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McIver, C.J.</creatorcontrib><creatorcontrib>Tapsall, J.W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McIver, C.J.</au><au>Tapsall, J.W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cysteine requirements of naturally occurring cysteine auxotrophs of Escherichia coli</atitle><jtitle>Pathology</jtitle><addtitle>Pathology</addtitle><date>1987</date><risdate>1987</risdate><volume>19</volume><issue>4</issue><spage>361</spage><epage>363</epage><pages>361-363</pages><issn>0031-3025</issn><eissn>1465-3931</eissn><abstract>The requirements for cysteine of naturally occurring cysteine auxotrophs of Escherichia coli were determined in a defined liquid medium. Maximal growth was obtained in the presence of cysteine concentrations between 20 and 250mg/l. At concentrations below 20mg/l growth of the auxotrophs, but not the prototrophic control, was suboptimal in this system. In the presence of cysteine concentrations in excess of 250mg/l, growth of both auxotrophic and prototrophic E. coli was inhibited with lower growth yields, a decreased specific growth rate and an extended lag phase being observed. These effects were minimised in the presence of 2mM L-leucine, L-isoleucine and L-valine.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>3328142</pmid><doi>10.3109/00313028709103884</doi><tpages>3</tpages></addata></record> |
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| ispartof | Pathology, 1987, Vol.19 (4), p.361-363 |
| issn | 0031-3025 1465-3931 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77985664 |
| source | MEDLINE; Access via Taylor & Francis; Alma/SFX Local Collection |
| subjects | Cysteine Cysteine - metabolism cystine E. coli Escherichia coli - growth & development Escherichia coli - metabolism Mutation nutritional requirement |
| title | Cysteine requirements of naturally occurring cysteine auxotrophs of Escherichia coli |
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