Isolation and primary culture of murine alveolar type II cells

Previous attempts to culture mouse alveolar type II (ATII) cells have been hampered by limited purity and cell recovery. We have now obtained culturable ATII cells from female C57BL/6 mice at a purity of 92% +/- 3 (mean +/- SD; n = 20), with viabilities of 96% +/- 2 and total yields of 5.1 +/- 0.7 X...

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Veröffentlicht in:	American journal of respiratory cell and molecular biology 1996-04, Vol.14 (4), p.309-315
Hauptverfasser:	Corti, M, Brody, AR, Harrison, JH
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Count Cell Culture Techniques Cell Separation Cell Survival Cells, Cultured Coloring Agents Culture Media Epithelial Cells Female Fibronectins Keratins - analysis Mice Mice, Inbred C57BL Microscopy, Electron Pulmonary Alveoli - cytology
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