Comparison of cytosolic levels of calcium and G actin in diffuse and localised adherent Escherichia coli-infected HeLa cells
Abstract In the present study we compared the intracellular level of free calcium ([Ca2+]i) and monomeric (G)/total (G + F) actin ratio in HeLa cells infected with diffuse (DAEC) and localised adherent Escherichia coli (LAEC). The level of [Ca2+]i was increased in both DAEC- and LAEC-infected HeLa c...
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| Veröffentlicht in: | FEMS microbiology letters 1996-01, Vol.135 (2-3), p.245-249 |
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| creator | Karmaker, Shyamali Ghosh Chaudhuri, Alok Ganguly, Uma |
| description | Abstract
In the present study we compared the intracellular level of free calcium ([Ca2+]i) and monomeric (G)/total (G + F) actin ratio in HeLa cells infected with diffuse (DAEC) and localised adherent Escherichia coli (LAEC). The level of [Ca2+]i was increased in both DAEC- and LAEC-infected HeLa cells. However, studies with EGTA- and dantrolene-treated cells and also suspension of cells in Ca2+-free buffer suggested that the rise of [Ca2+]i in DAEC-infected cells was due to the influx of Ca2+ from extracellular medium, whereas Ca2+ mobilisation from the intracellular stores was responsible for the enhancement of [Ca2+]i in LAEC-infected cells. It was also evident that the infection of HeLa cells with DAEC and LAEC caused alteration of G / G + F actin ratio as compared to that of control cells. The ratio was much lower in LAEC-infected cells than that of DAEC-infected ones. Moreover, cytochalasin B inhibited both DAEC and LAEC invasion to HeLa cells, suggesting further the role of microfilaments in the invasion process. |
| doi_str_mv | 10.1111/j.1574-6968.1996.tb07996.x |
| format | Article |
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In the present study we compared the intracellular level of free calcium ([Ca2+]i) and monomeric (G)/total (G + F) actin ratio in HeLa cells infected with diffuse (DAEC) and localised adherent Escherichia coli (LAEC). The level of [Ca2+]i was increased in both DAEC- and LAEC-infected HeLa cells. However, studies with EGTA- and dantrolene-treated cells and also suspension of cells in Ca2+-free buffer suggested that the rise of [Ca2+]i in DAEC-infected cells was due to the influx of Ca2+ from extracellular medium, whereas Ca2+ mobilisation from the intracellular stores was responsible for the enhancement of [Ca2+]i in LAEC-infected cells. It was also evident that the infection of HeLa cells with DAEC and LAEC caused alteration of G / G + F actin ratio as compared to that of control cells. The ratio was much lower in LAEC-infected cells than that of DAEC-infected ones. Moreover, cytochalasin B inhibited both DAEC and LAEC invasion to HeLa cells, suggesting further the role of microfilaments in the invasion process.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1996.tb07996.x</identifier><identifier>PMID: 8595864</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Actin ; Actins - metabolism ; Bacterial Adhesion ; Bacteriology ; Biological and medical sciences ; Calcium ; Calcium (extracellular) ; Calcium (intracellular) ; Calcium - metabolism ; Calcium buffering ; Calcium influx ; Calcium ions ; Calcium mobilization ; Cytochalasin B ; Cytochalasin B - pharmacology ; Cytosol - metabolism ; E coli ; Escherichia coli ; Escherichia coli - cytology ; Escherichia coli - pathogenicity ; Fundamental and applied biological sciences. Psychology ; HeLa cell ; HeLa Cells ; Humans ; Intracellular ; Intracellular calcium ion concentration ; Ion Transport ; Microbiology ; Microfilaments ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><ispartof>FEMS microbiology letters, 1996-01, Vol.135 (2-3), p.245-249</ispartof><rights>1996 Federation of European Microbiological Societies. All rights reserved 1996</rights><rights>1996 INIST-CNRS</rights><rights>1996 Federation of European Microbiological Societies. All rights reserved</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4355-a1b3a448420efa50ce98583fa933fee268cdbe594db05adbcfe2594d88fd3e0a3</citedby><cites>FETCH-LOGICAL-c4355-a1b3a448420efa50ce98583fa933fee268cdbe594db05adbcfe2594d88fd3e0a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1996.tb07996.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1996.tb07996.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2974383$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8595864$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karmaker, Shyamali</creatorcontrib><creatorcontrib>Ghosh Chaudhuri, Alok</creatorcontrib><creatorcontrib>Ganguly, Uma</creatorcontrib><title>Comparison of cytosolic levels of calcium and G actin in diffuse and localised adherent Escherichia coli-infected HeLa cells</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
In the present study we compared the intracellular level of free calcium ([Ca2+]i) and monomeric (G)/total (G + F) actin ratio in HeLa cells infected with diffuse (DAEC) and localised adherent Escherichia coli (LAEC). The level of [Ca2+]i was increased in both DAEC- and LAEC-infected HeLa cells. However, studies with EGTA- and dantrolene-treated cells and also suspension of cells in Ca2+-free buffer suggested that the rise of [Ca2+]i in DAEC-infected cells was due to the influx of Ca2+ from extracellular medium, whereas Ca2+ mobilisation from the intracellular stores was responsible for the enhancement of [Ca2+]i in LAEC-infected cells. It was also evident that the infection of HeLa cells with DAEC and LAEC caused alteration of G / G + F actin ratio as compared to that of control cells. The ratio was much lower in LAEC-infected cells than that of DAEC-infected ones. Moreover, cytochalasin B inhibited both DAEC and LAEC invasion to HeLa cells, suggesting further the role of microfilaments in the invasion process.</description><subject>Actin</subject><subject>Actins - metabolism</subject><subject>Bacterial Adhesion</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Calcium</subject><subject>Calcium (extracellular)</subject><subject>Calcium (intracellular)</subject><subject>Calcium - metabolism</subject><subject>Calcium buffering</subject><subject>Calcium influx</subject><subject>Calcium ions</subject><subject>Calcium mobilization</subject><subject>Cytochalasin B</subject><subject>Cytochalasin B - pharmacology</subject><subject>Cytosol - metabolism</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - cytology</subject><subject>Escherichia coli - pathogenicity</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa cell</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Intracellular</subject><subject>Intracellular calcium ion concentration</subject><subject>Ion Transport</subject><subject>Microbiology</subject><subject>Microfilaments</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkV2L1DAUhoMo67j6E4Sg4l3HpEmaxAtBhv0QRrzR65CmJ2yGthmbdt2B_fGb7pS5EEUMgSTnPOfNSV6E3lCypnl82K2pkLyodKXWVOtqPdZEzuvdE7Q6pZ6iFWFSFZRo-Ry9SGlHCOElqc7QmRJaqIqv0P0mdns7hBR7HD12hzGm2AaHW7iFNj3GbOvC1GHbN_gKWzeGHufZBO-nBI_hNmYoJGiwbW5ggH7EF8nlXXA3wWKXFYvQe3BjRq5hm0PQtukleuZtm-DVsp6jH5cX3zfXxfbb1ZfN523hOBOisLRmlnOVewdvBXGglVDMW82YBygr5ZoahOZNTYRtauehnE9K-YYBsewcvT_q7of4c4I0mi6kuQPbQ5ySkVIrqsrqnyCtuCBSyQy-_Q3cxWno8yNMyRhVRJbVTH08Um6IKQ3gzX4InR0OhhIzO2l2ZrbLzHaZ2UmzOGnucvHr5Yqp7qA5lS7W5fy7JW9T_n0_2N6FdMJKLTlTLGOfjtiv0MLhPxowl1-3JRdZQBwF4rT_S3nxp_4fAJ6QzDE</recordid><startdate>199601</startdate><enddate>199601</enddate><creator>Karmaker, Shyamali</creator><creator>Ghosh Chaudhuri, Alok</creator><creator>Ganguly, Uma</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199601</creationdate><title>Comparison of cytosolic levels of calcium and G actin in diffuse and localised adherent Escherichia coli-infected HeLa cells</title><author>Karmaker, Shyamali ; Ghosh Chaudhuri, Alok ; Ganguly, Uma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4355-a1b3a448420efa50ce98583fa933fee268cdbe594db05adbcfe2594d88fd3e0a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Actin</topic><topic>Actins - metabolism</topic><topic>Bacterial Adhesion</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Calcium</topic><topic>Calcium (extracellular)</topic><topic>Calcium (intracellular)</topic><topic>Calcium - metabolism</topic><topic>Calcium buffering</topic><topic>Calcium influx</topic><topic>Calcium ions</topic><topic>Calcium mobilization</topic><topic>Cytochalasin B</topic><topic>Cytochalasin B - pharmacology</topic><topic>Cytosol - metabolism</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - cytology</topic><topic>Escherichia coli - pathogenicity</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa cell</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Intracellular</topic><topic>Intracellular calcium ion concentration</topic><topic>Ion Transport</topic><topic>Microbiology</topic><topic>Microfilaments</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karmaker, Shyamali</creatorcontrib><creatorcontrib>Ghosh Chaudhuri, Alok</creatorcontrib><creatorcontrib>Ganguly, Uma</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karmaker, Shyamali</au><au>Ghosh Chaudhuri, Alok</au><au>Ganguly, Uma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of cytosolic levels of calcium and G actin in diffuse and localised adherent Escherichia coli-infected HeLa cells</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1996-01</date><risdate>1996</risdate><volume>135</volume><issue>2-3</issue><spage>245</spage><epage>249</epage><pages>245-249</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
In the present study we compared the intracellular level of free calcium ([Ca2+]i) and monomeric (G)/total (G + F) actin ratio in HeLa cells infected with diffuse (DAEC) and localised adherent Escherichia coli (LAEC). The level of [Ca2+]i was increased in both DAEC- and LAEC-infected HeLa cells. However, studies with EGTA- and dantrolene-treated cells and also suspension of cells in Ca2+-free buffer suggested that the rise of [Ca2+]i in DAEC-infected cells was due to the influx of Ca2+ from extracellular medium, whereas Ca2+ mobilisation from the intracellular stores was responsible for the enhancement of [Ca2+]i in LAEC-infected cells. It was also evident that the infection of HeLa cells with DAEC and LAEC caused alteration of G / G + F actin ratio as compared to that of control cells. The ratio was much lower in LAEC-infected cells than that of DAEC-infected ones. Moreover, cytochalasin B inhibited both DAEC and LAEC invasion to HeLa cells, suggesting further the role of microfilaments in the invasion process.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8595864</pmid><doi>10.1111/j.1574-6968.1996.tb07996.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1996-01, Vol.135 (2-3), p.245-249 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77981826 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Actin Actins - metabolism Bacterial Adhesion Bacteriology Biological and medical sciences Calcium Calcium (extracellular) Calcium (intracellular) Calcium - metabolism Calcium buffering Calcium influx Calcium ions Calcium mobilization Cytochalasin B Cytochalasin B - pharmacology Cytosol - metabolism E coli Escherichia coli Escherichia coli - cytology Escherichia coli - pathogenicity Fundamental and applied biological sciences. Psychology HeLa cell HeLa Cells Humans Intracellular Intracellular calcium ion concentration Ion Transport Microbiology Microfilaments Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains |
| title | Comparison of cytosolic levels of calcium and G actin in diffuse and localised adherent Escherichia coli-infected HeLa cells |
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