A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM‐1 is a ligand for CD11c/CD18
The rabbit CD11c molecule has been characterized by use of a new monoclonal antibody, mAb 3/22. Expression of the p150,95 integrin (CD11c/CD18) has been shown by flow cytometry and immunohistochemistry to be restricted to monocytes, macrophages, dendritic cells and a small population of lymphocytes...
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| Veröffentlicht in: | European journal of immunology 1996-03, Vol.26 (3), p.525-531 |
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| container_title | European journal of immunology |
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| creator | Blackford, Jeanette Reid, Hugh W. Pappin, Darryl J. C. Bowers, Florence S. Wilkinson, J. Michael |
| description | The rabbit CD11c molecule has been characterized by use of a new monoclonal antibody, mAb 3/22. Expression of the p150,95 integrin (CD11c/CD18) has been shown by flow cytometry and immunohistochemistry to be restricted to monocytes, macrophages, dendritic cells and a small population of lymphocytes in peripheral blood. No expression on neutrophils could be demonstrated. Incubation of the newly derived CD8+ T cell line, BJ/873, with mAb 3/22 causes homotypic aggregation, which has been shown to be a cell surface event that is not dependent on intracellular signaling or on receptor cross‐linking. Inhibition studies show that the ligands responsible for this aggregation are CD11c/CD 18 and ICAM‐1, both of which are expressed on BJ/873. One other rabbit T cell line, K34, that also expresses p150,95 and ICAM‐1, shows a similar aggregation response when stimulated with 3/22. Cell lines that express p150,95 but not ICAM‐1 do not aggregate. These observations suggest that ICAM‐1 is a ligand for activated p150,95. |
| doi_str_mv | 10.1002/eji.1830260304 |
| format | Article |
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C. ; Bowers, Florence S. ; Wilkinson, J. Michael</creator><creatorcontrib>Blackford, Jeanette ; Reid, Hugh W. ; Pappin, Darryl J. C. ; Bowers, Florence S. ; Wilkinson, J. Michael</creatorcontrib><description>The rabbit CD11c molecule has been characterized by use of a new monoclonal antibody, mAb 3/22. Expression of the p150,95 integrin (CD11c/CD18) has been shown by flow cytometry and immunohistochemistry to be restricted to monocytes, macrophages, dendritic cells and a small population of lymphocytes in peripheral blood. No expression on neutrophils could be demonstrated. Incubation of the newly derived CD8+ T cell line, BJ/873, with mAb 3/22 causes homotypic aggregation, which has been shown to be a cell surface event that is not dependent on intracellular signaling or on receptor cross‐linking. Inhibition studies show that the ligands responsible for this aggregation are CD11c/CD 18 and ICAM‐1, both of which are expressed on BJ/873. One other rabbit T cell line, K34, that also expresses p150,95 and ICAM‐1, shows a similar aggregation response when stimulated with 3/22. Cell lines that express p150,95 but not ICAM‐1 do not aggregate. These observations suggest that ICAM‐1 is a ligand for activated p150,95.</description><identifier>ISSN: 0014-2980</identifier><identifier>EISSN: 1521-4141</identifier><identifier>DOI: 10.1002/eji.1830260304</identifier><identifier>PMID: 8605916</identifier><language>eng</language><publisher>Weinheim: WILEY‐VCH Verlag GmbH</publisher><subject>Adhesion ; Amino Acid Sequence ; Animals ; Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - pharmacology ; Antigen-Antibody Reactions ; Binding, Competitive - immunology ; Cations, Divalent ; CD11c ; CD18 Antigens - immunology ; Cell Aggregation - immunology ; Cell Line ; Immunophenotyping ; Integrin ; Integrin alphaXbeta2 - immunology ; Integrin alphaXbeta2 - metabolism ; Intercellular Adhesion Molecule-1 - immunology ; Ligands ; Male ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Monoclonal antibody ; Rabbit ; Rabbits ; Signal Transduction - immunology ; T-Lymphocytes - immunology ; Temperature</subject><ispartof>European journal of immunology, 1996-03, Vol.26 (3), p.525-531</ispartof><rights>Copyright © 1996 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4114-335542ad2dd0eb98283efc5df099007b5a4c1d848d111773cf109a03f1114eca3</citedby><cites>FETCH-LOGICAL-c4114-335542ad2dd0eb98283efc5df099007b5a4c1d848d111773cf109a03f1114eca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Feji.1830260304$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Feji.1830260304$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8605916$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blackford, Jeanette</creatorcontrib><creatorcontrib>Reid, Hugh W.</creatorcontrib><creatorcontrib>Pappin, Darryl J. C.</creatorcontrib><creatorcontrib>Bowers, Florence S.</creatorcontrib><creatorcontrib>Wilkinson, J. Michael</creatorcontrib><title>A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM‐1 is a ligand for CD11c/CD18</title><title>European journal of immunology</title><addtitle>Eur J Immunol</addtitle><description>The rabbit CD11c molecule has been characterized by use of a new monoclonal antibody, mAb 3/22. Expression of the p150,95 integrin (CD11c/CD18) has been shown by flow cytometry and immunohistochemistry to be restricted to monocytes, macrophages, dendritic cells and a small population of lymphocytes in peripheral blood. No expression on neutrophils could be demonstrated. Incubation of the newly derived CD8+ T cell line, BJ/873, with mAb 3/22 causes homotypic aggregation, which has been shown to be a cell surface event that is not dependent on intracellular signaling or on receptor cross‐linking. Inhibition studies show that the ligands responsible for this aggregation are CD11c/CD 18 and ICAM‐1, both of which are expressed on BJ/873. One other rabbit T cell line, K34, that also expresses p150,95 and ICAM‐1, shows a similar aggregation response when stimulated with 3/22. Cell lines that express p150,95 but not ICAM‐1 do not aggregate. These observations suggest that ICAM‐1 is a ligand for activated p150,95.</description><subject>Adhesion</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - pharmacology</subject><subject>Antigen-Antibody Reactions</subject><subject>Binding, Competitive - immunology</subject><subject>Cations, Divalent</subject><subject>CD11c</subject><subject>CD18 Antigens - immunology</subject><subject>Cell Aggregation - immunology</subject><subject>Cell Line</subject><subject>Immunophenotyping</subject><subject>Integrin</subject><subject>Integrin alphaXbeta2 - immunology</subject><subject>Integrin alphaXbeta2 - metabolism</subject><subject>Intercellular Adhesion Molecule-1 - immunology</subject><subject>Ligands</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Sequence Data</subject><subject>Monoclonal antibody</subject><subject>Rabbit</subject><subject>Rabbits</subject><subject>Signal Transduction - immunology</subject><subject>T-Lymphocytes - immunology</subject><subject>Temperature</subject><issn>0014-2980</issn><issn>1521-4141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvEzEQhS0EKqFw5YY0J05NMmN7d21uVShQVMSlnFde25u42l2H9aZVbpw58Rv5JThKBNwqS7ZG771PYz3GXhMuCJEv_V1YkBLISxQon7AZFZzmkiQ9ZTNEknOuFT5nL1K6Q0RdFvqMnakSC03ljP28hD4O0XZxMB2YYQpNdPsLEEvOL2CKMJqmCROs3hNZeNgEu4EwuJ31CTaxj9N-GyzcgvVdjq_Xo1-bKcThHfj74PxgPUwbM8H16vLL7x-_CEICA11Ym8FBG8cjeJlv9ZI9a02X_KvTe86-fbi6XX2a33z9mOM3cyspf0eIopDcOO4c-kYrroRvbeFa1BqxagojLTkllSOiqhK2JdQGRZtH6a0R5-ztkbsd4_edT1Pdh3TY3ww-7lJdVfpw6FEjFWVZSamycXE02jGmNPq23o6hN-O-JqwPLdW5pfpfSznw5kTeNb13f-2nWrKuj_pD6Pz-EVp99fn6P_Yfk3ObbQ</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Blackford, Jeanette</creator><creator>Reid, Hugh W.</creator><creator>Pappin, Darryl J. C.</creator><creator>Bowers, Florence S.</creator><creator>Wilkinson, J. Michael</creator><general>WILEY‐VCH Verlag GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19960301</creationdate><title>A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM‐1 is a ligand for CD11c/CD18</title><author>Blackford, Jeanette ; Reid, Hugh W. ; Pappin, Darryl J. C. ; Bowers, Florence S. ; Wilkinson, J. Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4114-335542ad2dd0eb98283efc5df099007b5a4c1d848d111773cf109a03f1114eca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adhesion</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - pharmacology</topic><topic>Antigen-Antibody Reactions</topic><topic>Binding, Competitive - immunology</topic><topic>Cations, Divalent</topic><topic>CD11c</topic><topic>CD18 Antigens - immunology</topic><topic>Cell Aggregation - immunology</topic><topic>Cell Line</topic><topic>Immunophenotyping</topic><topic>Integrin</topic><topic>Integrin alphaXbeta2 - immunology</topic><topic>Integrin alphaXbeta2 - metabolism</topic><topic>Intercellular Adhesion Molecule-1 - immunology</topic><topic>Ligands</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Sequence Data</topic><topic>Monoclonal antibody</topic><topic>Rabbit</topic><topic>Rabbits</topic><topic>Signal Transduction - immunology</topic><topic>T-Lymphocytes - immunology</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blackford, Jeanette</creatorcontrib><creatorcontrib>Reid, Hugh W.</creatorcontrib><creatorcontrib>Pappin, Darryl J. C.</creatorcontrib><creatorcontrib>Bowers, Florence S.</creatorcontrib><creatorcontrib>Wilkinson, J. Michael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blackford, Jeanette</au><au>Reid, Hugh W.</au><au>Pappin, Darryl J. C.</au><au>Bowers, Florence S.</au><au>Wilkinson, J. Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM‐1 is a ligand for CD11c/CD18</atitle><jtitle>European journal of immunology</jtitle><addtitle>Eur J Immunol</addtitle><date>1996-03-01</date><risdate>1996</risdate><volume>26</volume><issue>3</issue><spage>525</spage><epage>531</epage><pages>525-531</pages><issn>0014-2980</issn><eissn>1521-4141</eissn><abstract>The rabbit CD11c molecule has been characterized by use of a new monoclonal antibody, mAb 3/22. Expression of the p150,95 integrin (CD11c/CD18) has been shown by flow cytometry and immunohistochemistry to be restricted to monocytes, macrophages, dendritic cells and a small population of lymphocytes in peripheral blood. No expression on neutrophils could be demonstrated. Incubation of the newly derived CD8+ T cell line, BJ/873, with mAb 3/22 causes homotypic aggregation, which has been shown to be a cell surface event that is not dependent on intracellular signaling or on receptor cross‐linking. Inhibition studies show that the ligands responsible for this aggregation are CD11c/CD 18 and ICAM‐1, both of which are expressed on BJ/873. One other rabbit T cell line, K34, that also expresses p150,95 and ICAM‐1, shows a similar aggregation response when stimulated with 3/22. Cell lines that express p150,95 but not ICAM‐1 do not aggregate. These observations suggest that ICAM‐1 is a ligand for activated p150,95.</abstract><cop>Weinheim</cop><pub>WILEY‐VCH Verlag GmbH</pub><pmid>8605916</pmid><doi>10.1002/eji.1830260304</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adhesion Amino Acid Sequence Animals Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - pharmacology Antigen-Antibody Reactions Binding, Competitive - immunology Cations, Divalent CD11c CD18 Antigens - immunology Cell Aggregation - immunology Cell Line Immunophenotyping Integrin Integrin alphaXbeta2 - immunology Integrin alphaXbeta2 - metabolism Intercellular Adhesion Molecule-1 - immunology Ligands Male Mice Mice, Inbred BALB C Molecular Sequence Data Monoclonal antibody Rabbit Rabbits Signal Transduction - immunology T-Lymphocytes - immunology Temperature |
| title | A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: evidence that ICAM‐1 is a ligand for CD11c/CD18 |
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