Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin
A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the determination of the concentration of ivermectin (IVM) in biological fluids was developed. A conjugate of IVM on bovine serum albumin and poly-L-lysine was used to produce antibodies in rabbits and served as a solid-phase...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 1996-03, Vol.54 (3), p.243-248 |
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| container_title | The American journal of tropical medicine and hygiene |
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| creator | Mitsui, Yoshinori Tanimori, Hideaki Kitagawa, Tsunehiro Fujimaki, Yasunori Aoki, Yoshiki |
| description | A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the determination of the concentration of ivermectin (IVM) in biological fluids was developed. A conjugate of IVM on bovine serum albumin and poly-L-lysine was used to produce antibodies in rabbits and served as a solid-phase marker for titration of antibodies, respectively. The competitive ELISA was conducted by simultaneously incubating IVM and IVM-biotin conjugate with anti-IVM antiserum over goat anti-rabbit IgG (Fc) and then determining the amount of bound IVM-biotin with avidin-peroxidase conjugate as a tracer. The coefficient of variation for the assay was less than 10% in the range of 0.3-10 ng/ml. The limit of detection was 0.1 ng/ml. The cross-reactivities of anti-IVM antiserum with some anthelmintic drugs were negligible. Using this ELISA, serum levels of IVM were easily determined in Mongolian jirds (Meriones unguiculatus) up to 72 hr following a single oral dose of 500 microgram/kg of body weight. |
| doi_str_mv | 10.4269/ajtmh.1996.54.243 |
| format | Article |
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A conjugate of IVM on bovine serum albumin and poly-L-lysine was used to produce antibodies in rabbits and served as a solid-phase marker for titration of antibodies, respectively. The competitive ELISA was conducted by simultaneously incubating IVM and IVM-biotin conjugate with anti-IVM antiserum over goat anti-rabbit IgG (Fc) and then determining the amount of bound IVM-biotin with avidin-peroxidase conjugate as a tracer. The coefficient of variation for the assay was less than 10% in the range of 0.3-10 ng/ml. The limit of detection was 0.1 ng/ml. The cross-reactivities of anti-IVM antiserum with some anthelmintic drugs were negligible. Using this ELISA, serum levels of IVM were easily determined in Mongolian jirds (Meriones unguiculatus) up to 72 hr following a single oral dose of 500 microgram/kg of body weight.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.1996.54.243</identifier><identifier>PMID: 8600758</identifier><identifier>CODEN: AJTHAB</identifier><language>eng</language><publisher>Lawrence, KS: ASTMH</publisher><subject>Animals ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Antibody Formation ; Antiparasitic agents ; Antiparasitic Agents - analysis ; Antiparasitic Agents - immunology ; Antiparasitic Agents - pharmacokinetics ; Avidin ; Binding, Competitive ; Biological and medical sciences ; Biotin ; Cross Reactions ; Dose-Response Relationship, Immunologic ; Enzyme-Linked Immunosorbent Assay ; Gerbillinae ; Immunoglobulin G ; Ivermectin - analysis ; Ivermectin - immunology ; Ivermectin - pharmacokinetics ; Male ; Medical sciences ; Peroxidase ; Pharmacology. Drug treatments ; Rabbits ; Sensitivity and Specificity ; Serum Albumin, Bovine ; Tropical medicine</subject><ispartof>The American journal of tropical medicine and hygiene, 1996-03, Vol.54 (3), p.243-248</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-33db825a3498884209e67cb357fb6bcb4ecc023299965debf281aee5a00b9c2c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3037969$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8600758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitsui, Yoshinori</creatorcontrib><creatorcontrib>Tanimori, Hideaki</creatorcontrib><creatorcontrib>Kitagawa, Tsunehiro</creatorcontrib><creatorcontrib>Fujimaki, Yasunori</creatorcontrib><creatorcontrib>Aoki, Yoshiki</creatorcontrib><title>Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the determination of the concentration of ivermectin (IVM) in biological fluids was developed. A conjugate of IVM on bovine serum albumin and poly-L-lysine was used to produce antibodies in rabbits and served as a solid-phase marker for titration of antibodies, respectively. The competitive ELISA was conducted by simultaneously incubating IVM and IVM-biotin conjugate with anti-IVM antiserum over goat anti-rabbit IgG (Fc) and then determining the amount of bound IVM-biotin with avidin-peroxidase conjugate as a tracer. The coefficient of variation for the assay was less than 10% in the range of 0.3-10 ng/ml. The limit of detection was 0.1 ng/ml. The cross-reactivities of anti-IVM antiserum with some anthelmintic drugs were negligible. Using this ELISA, serum levels of IVM were easily determined in Mongolian jirds (Meriones unguiculatus) up to 72 hr following a single oral dose of 500 microgram/kg of body weight.</description><subject>Animals</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antibody Formation</subject><subject>Antiparasitic agents</subject><subject>Antiparasitic Agents - analysis</subject><subject>Antiparasitic Agents - immunology</subject><subject>Antiparasitic Agents - pharmacokinetics</subject><subject>Avidin</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Biotin</subject><subject>Cross Reactions</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Gerbillinae</subject><subject>Immunoglobulin G</subject><subject>Ivermectin - analysis</subject><subject>Ivermectin - immunology</subject><subject>Ivermectin - pharmacokinetics</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Peroxidase</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbits</subject><subject>Sensitivity and Specificity</subject><subject>Serum Albumin, Bovine</subject><subject>Tropical medicine</subject><issn>0002-9637</issn><issn>1476-1645</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1vEzEQhq0KVELpD-CAtAfgtsFf649jVQWIFIlDy9myvbPEZe0N9qZR-utxm6inOczzvqN5EPpI8JJTob_Zhzlul0Rrsez4knJ2gRaES9ESwbs3aIExpq0WTL5D70t5wJgoSsglulQCY9mpBVrdhbgbobGpb-4glTCHR2hW6ekYod2E9Bf6Zh3jPk1lyg7S3NyUYo_NMOVm_Qg5gp9D-oDeDnYscH2eV-j399X97c928-vH-vZm03rO6dwy1jtFO8u4VkpxijUI6R3r5OCE846D95gyqutDXQ9uoIpYgM5i7LSnnl2hr6feXZ7-7aHMJobiYRxtgmlfjJRaqvp_BckJ9HkqJcNgdjlEm4-GYPOszryoM8_qTMdNVVczn87lexehf02cXdX95_PeFm_HIdvkQ3nFGGZSC12xLydsG_5sDyGDKdGOYy0l5nA41GPs5dx_7nGFAw</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Mitsui, Yoshinori</creator><creator>Tanimori, Hideaki</creator><creator>Kitagawa, Tsunehiro</creator><creator>Fujimaki, Yasunori</creator><creator>Aoki, Yoshiki</creator><general>ASTMH</general><general>Allen Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960301</creationdate><title>Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin</title><author>Mitsui, Yoshinori ; Tanimori, Hideaki ; Kitagawa, Tsunehiro ; Fujimaki, Yasunori ; Aoki, Yoshiki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-33db825a3498884209e67cb357fb6bcb4ecc023299965debf281aee5a00b9c2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Antibody Formation</topic><topic>Antiparasitic agents</topic><topic>Antiparasitic Agents - analysis</topic><topic>Antiparasitic Agents - immunology</topic><topic>Antiparasitic Agents - pharmacokinetics</topic><topic>Avidin</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Biotin</topic><topic>Cross Reactions</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Gerbillinae</topic><topic>Immunoglobulin G</topic><topic>Ivermectin - analysis</topic><topic>Ivermectin - immunology</topic><topic>Ivermectin - pharmacokinetics</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Peroxidase</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbits</topic><topic>Sensitivity and Specificity</topic><topic>Serum Albumin, Bovine</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitsui, Yoshinori</creatorcontrib><creatorcontrib>Tanimori, Hideaki</creatorcontrib><creatorcontrib>Kitagawa, Tsunehiro</creatorcontrib><creatorcontrib>Fujimaki, Yasunori</creatorcontrib><creatorcontrib>Aoki, Yoshiki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The American journal of tropical medicine and hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitsui, Yoshinori</au><au>Tanimori, Hideaki</au><au>Kitagawa, Tsunehiro</au><au>Fujimaki, Yasunori</au><au>Aoki, Yoshiki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin</atitle><jtitle>The American journal of tropical medicine and hygiene</jtitle><addtitle>Am J Trop Med Hyg</addtitle><date>1996-03-01</date><risdate>1996</risdate><volume>54</volume><issue>3</issue><spage>243</spage><epage>248</epage><pages>243-248</pages><issn>0002-9637</issn><eissn>1476-1645</eissn><coden>AJTHAB</coden><abstract>A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the determination of the concentration of ivermectin (IVM) in biological fluids was developed. A conjugate of IVM on bovine serum albumin and poly-L-lysine was used to produce antibodies in rabbits and served as a solid-phase marker for titration of antibodies, respectively. The competitive ELISA was conducted by simultaneously incubating IVM and IVM-biotin conjugate with anti-IVM antiserum over goat anti-rabbit IgG (Fc) and then determining the amount of bound IVM-biotin with avidin-peroxidase conjugate as a tracer. The coefficient of variation for the assay was less than 10% in the range of 0.3-10 ng/ml. The limit of detection was 0.1 ng/ml. The cross-reactivities of anti-IVM antiserum with some anthelmintic drugs were negligible. Using this ELISA, serum levels of IVM were easily determined in Mongolian jirds (Meriones unguiculatus) up to 72 hr following a single oral dose of 500 microgram/kg of body weight.</abstract><cop>Lawrence, KS</cop><pub>ASTMH</pub><pmid>8600758</pmid><doi>10.4269/ajtmh.1996.54.243</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0002-9637 |
| ispartof | The American journal of tropical medicine and hygiene, 1996-03, Vol.54 (3), p.243-248 |
| issn | 0002-9637 1476-1645 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antibody Formation Antiparasitic agents Antiparasitic Agents - analysis Antiparasitic Agents - immunology Antiparasitic Agents - pharmacokinetics Avidin Binding, Competitive Biological and medical sciences Biotin Cross Reactions Dose-Response Relationship, Immunologic Enzyme-Linked Immunosorbent Assay Gerbillinae Immunoglobulin G Ivermectin - analysis Ivermectin - immunology Ivermectin - pharmacokinetics Male Medical sciences Peroxidase Pharmacology. Drug treatments Rabbits Sensitivity and Specificity Serum Albumin, Bovine Tropical medicine |
| title | Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin |
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