Strand displacement amplification (SDA) and transient-state fluorescence polarization detection of Mycobacterium tuberculosis DNA

Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a DNA sequence for diagnostic purposes. We have combined SDA with fluorescence polarization detection in a closed, homogeneous format. A fluorescently labeled oligodeoxynucleotide detector probe hybridizes to the...

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Veröffentlicht in:	Clinical chemistry (Baltimore, Md.) Md.), 1996-01, Vol.42 (1), p.9-13
Hauptverfasser:	Walker, GT, Nadeau, JG, Linn, CP, Devlin, RF, Dandliker, WB
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Biotechnology DNA Primers DNA Probes DNA, Bacterial - analysis False Positive Reactions Fluorescence Polarization Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics In vitro gene amplification. Pcr technique Methods. Procedures. Technologies Molecular Sequence Data Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Nucleic Acid Amplification Techniques
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