Surfactant treatment before reperfusion improves the immediate function of lung transplants in rats
An impaired function of alveolar surfactant can cause lung transplant dysfunction early after reperfusion. In this study it was investigated whether treatment with surfactant before reperfusion improves the immediate function of lung transplants and whether an improved transplant function was associ...
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| Veröffentlicht in: | American journal of respiratory and critical care medicine 1996-02, Vol.153 (2), p.665-670 |
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| container_title | American journal of respiratory and critical care medicine |
| container_volume | 153 |
| creator | ERASMUS, M. E PETERSEN, A. H HOFSTEDE, G HAAGSMAN, H. P OETOMO, S. B PROP, J |
| description | An impaired function of alveolar surfactant can cause lung transplant dysfunction early after reperfusion. In this study it was investigated whether treatment with surfactant before reperfusion improves the immediate function of lung transplants and whether an improved transplant function was associated with an increase in alveolar surfactant components. Left lungs with 6-h (n = 8) or prolonged 20-h ischemia (n = 10) were transplanted syngeneically in rats. In both ischemia groups half of the lung transplants were treated with surfactant just before reperfusion. Lung function was measured during reperfusion for 1 h. Thereafter, the rats were killed and bronchoalveolar lavage was performed to measure alveolar surfactant components. We found that surfactant treatment improved the immediate function of lung transplants in parallel with a higher amount of total surfactant phospholipids, a higher percentage of the heavy subtype of surfactant, a normalized percentage of phosphatidylcholine, and a higher amount of endogenous surfactant protein A (SP-A). We conclude that surfactant treatment before reperfusion does improve the immediate lung transplant function in rats in association with an increase in alveolar surfactant components. More particularly, the amount of (endogenous) SP-A is thought to be crucial for the efficacy of surfactant treatment after lung transplantation. |
| doi_str_mv | 10.1164/ajrccm.153.2.8564115 |
| format | Article |
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E ; PETERSEN, A. H ; HOFSTEDE, G ; HAAGSMAN, H. P ; OETOMO, S. B ; PROP, J</creator><creatorcontrib>ERASMUS, M. E ; PETERSEN, A. H ; HOFSTEDE, G ; HAAGSMAN, H. P ; OETOMO, S. B ; PROP, J</creatorcontrib><description>An impaired function of alveolar surfactant can cause lung transplant dysfunction early after reperfusion. In this study it was investigated whether treatment with surfactant before reperfusion improves the immediate function of lung transplants and whether an improved transplant function was associated with an increase in alveolar surfactant components. Left lungs with 6-h (n = 8) or prolonged 20-h ischemia (n = 10) were transplanted syngeneically in rats. In both ischemia groups half of the lung transplants were treated with surfactant just before reperfusion. Lung function was measured during reperfusion for 1 h. Thereafter, the rats were killed and bronchoalveolar lavage was performed to measure alveolar surfactant components. We found that surfactant treatment improved the immediate function of lung transplants in parallel with a higher amount of total surfactant phospholipids, a higher percentage of the heavy subtype of surfactant, a normalized percentage of phosphatidylcholine, and a higher amount of endogenous surfactant protein A (SP-A). We conclude that surfactant treatment before reperfusion does improve the immediate lung transplant function in rats in association with an increase in alveolar surfactant components. More particularly, the amount of (endogenous) SP-A is thought to be crucial for the efficacy of surfactant treatment after lung transplantation.</description><identifier>ISSN: 1073-449X</identifier><identifier>EISSN: 1535-4970</identifier><identifier>DOI: 10.1164/ajrccm.153.2.8564115</identifier><identifier>PMID: 8564115</identifier><language>eng</language><publisher>New York, NY: American Lung Association</publisher><subject>Animals ; Biological and medical sciences ; Blood Proteins - analysis ; Bronchoalveolar Lavage Fluid - chemistry ; Glycoproteins - analysis ; In Vitro Techniques ; L-Lactate Dehydrogenase - analysis ; Lipids - pharmacology ; Lung - physiopathology ; Lung Transplantation ; Male ; Medical sciences ; Phospholipids - analysis ; Proteolipids - analysis ; Pulmonary Circulation ; Pulmonary Surfactant-Associated Protein A ; Pulmonary Surfactant-Associated Proteins ; Pulmonary Surfactants - analysis ; Pulmonary Surfactants - pharmacology ; Rats ; Rats, Inbred Lew ; Reperfusion ; Surface Tension ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the respiratory system</subject><ispartof>American journal of respiratory and critical care medicine, 1996-02, Vol.153 (2), p.665-670</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c331t-ae8c94ef6ddd0563564d973656d3880660b12ad32698c41410ad80580d77f7003</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2989539$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8564115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ERASMUS, M. E</creatorcontrib><creatorcontrib>PETERSEN, A. H</creatorcontrib><creatorcontrib>HOFSTEDE, G</creatorcontrib><creatorcontrib>HAAGSMAN, H. P</creatorcontrib><creatorcontrib>OETOMO, S. B</creatorcontrib><creatorcontrib>PROP, J</creatorcontrib><title>Surfactant treatment before reperfusion improves the immediate function of lung transplants in rats</title><title>American journal of respiratory and critical care medicine</title><addtitle>Am J Respir Crit Care Med</addtitle><description>An impaired function of alveolar surfactant can cause lung transplant dysfunction early after reperfusion. In this study it was investigated whether treatment with surfactant before reperfusion improves the immediate function of lung transplants and whether an improved transplant function was associated with an increase in alveolar surfactant components. Left lungs with 6-h (n = 8) or prolonged 20-h ischemia (n = 10) were transplanted syngeneically in rats. In both ischemia groups half of the lung transplants were treated with surfactant just before reperfusion. Lung function was measured during reperfusion for 1 h. Thereafter, the rats were killed and bronchoalveolar lavage was performed to measure alveolar surfactant components. We found that surfactant treatment improved the immediate function of lung transplants in parallel with a higher amount of total surfactant phospholipids, a higher percentage of the heavy subtype of surfactant, a normalized percentage of phosphatidylcholine, and a higher amount of endogenous surfactant protein A (SP-A). We conclude that surfactant treatment before reperfusion does improve the immediate lung transplant function in rats in association with an increase in alveolar surfactant components. More particularly, the amount of (endogenous) SP-A is thought to be crucial for the efficacy of surfactant treatment after lung transplantation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Proteins - analysis</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Glycoproteins - analysis</subject><subject>In Vitro Techniques</subject><subject>L-Lactate Dehydrogenase - analysis</subject><subject>Lipids - pharmacology</subject><subject>Lung - physiopathology</subject><subject>Lung Transplantation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Phospholipids - analysis</subject><subject>Proteolipids - analysis</subject><subject>Pulmonary Circulation</subject><subject>Pulmonary Surfactant-Associated Protein A</subject><subject>Pulmonary Surfactant-Associated Proteins</subject><subject>Pulmonary Surfactants - analysis</subject><subject>Pulmonary Surfactants - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Reperfusion</subject><subject>Surface Tension</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Surgery of the respiratory system</subject><issn>1073-449X</issn><issn>1535-4970</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLAzEUhYMotVb_gcIsxN3UZJJJMkspvqDgQgV3IU1udMq8TDKC_96UDnWVeznnfpwchC4JXhLC2a3eemPaJSnpsljKkjNCyiM0T3uZs0rg4zRjQXPGqo9TdBbCFmNSSIJnaDbZ58i8jt5pE3UXs-hBxxbStAHXe8g8DODdGOq-y-p28P0PhCx-QVpasLWOkLmxM3Gn9y5rxu4zQXQXhibxQlZ3mdcxnKMTp5sAF9O7QO8P92-rp3z98vi8ulvnhlIScw3SVAwct9biktMU0VaC8pJbKiXmHG9IoS0teCUNI4xgbSUuJbZCOIExXaCbPTcl_R4hRNXWwUCTwkA_BiVEwhWCJyPbG43vQ_Dg1ODrVvtfRbDadav23arUpSrUVFY6u5r44yb9_3D0r19Pug5GNy41YepwsBWVrEpa0T_aoYSW</recordid><startdate>19960201</startdate><enddate>19960201</enddate><creator>ERASMUS, M. E</creator><creator>PETERSEN, A. H</creator><creator>HOFSTEDE, G</creator><creator>HAAGSMAN, H. P</creator><creator>OETOMO, S. B</creator><creator>PROP, J</creator><general>American Lung Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960201</creationdate><title>Surfactant treatment before reperfusion improves the immediate function of lung transplants in rats</title><author>ERASMUS, M. E ; PETERSEN, A. H ; HOFSTEDE, G ; HAAGSMAN, H. P ; OETOMO, S. B ; PROP, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c331t-ae8c94ef6ddd0563564d973656d3880660b12ad32698c41410ad80580d77f7003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Proteins - analysis</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Glycoproteins - analysis</topic><topic>In Vitro Techniques</topic><topic>L-Lactate Dehydrogenase - analysis</topic><topic>Lipids - pharmacology</topic><topic>Lung - physiopathology</topic><topic>Lung Transplantation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Phospholipids - analysis</topic><topic>Proteolipids - analysis</topic><topic>Pulmonary Circulation</topic><topic>Pulmonary Surfactant-Associated Protein A</topic><topic>Pulmonary Surfactant-Associated Proteins</topic><topic>Pulmonary Surfactants - analysis</topic><topic>Pulmonary Surfactants - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Reperfusion</topic><topic>Surface Tension</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Surgery of the respiratory system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ERASMUS, M. E</creatorcontrib><creatorcontrib>PETERSEN, A. H</creatorcontrib><creatorcontrib>HOFSTEDE, G</creatorcontrib><creatorcontrib>HAAGSMAN, H. P</creatorcontrib><creatorcontrib>OETOMO, S. B</creatorcontrib><creatorcontrib>PROP, J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of respiratory and critical care medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ERASMUS, M. E</au><au>PETERSEN, A. H</au><au>HOFSTEDE, G</au><au>HAAGSMAN, H. P</au><au>OETOMO, S. B</au><au>PROP, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surfactant treatment before reperfusion improves the immediate function of lung transplants in rats</atitle><jtitle>American journal of respiratory and critical care medicine</jtitle><addtitle>Am J Respir Crit Care Med</addtitle><date>1996-02-01</date><risdate>1996</risdate><volume>153</volume><issue>2</issue><spage>665</spage><epage>670</epage><pages>665-670</pages><issn>1073-449X</issn><eissn>1535-4970</eissn><abstract>An impaired function of alveolar surfactant can cause lung transplant dysfunction early after reperfusion. In this study it was investigated whether treatment with surfactant before reperfusion improves the immediate function of lung transplants and whether an improved transplant function was associated with an increase in alveolar surfactant components. Left lungs with 6-h (n = 8) or prolonged 20-h ischemia (n = 10) were transplanted syngeneically in rats. In both ischemia groups half of the lung transplants were treated with surfactant just before reperfusion. Lung function was measured during reperfusion for 1 h. Thereafter, the rats were killed and bronchoalveolar lavage was performed to measure alveolar surfactant components. We found that surfactant treatment improved the immediate function of lung transplants in parallel with a higher amount of total surfactant phospholipids, a higher percentage of the heavy subtype of surfactant, a normalized percentage of phosphatidylcholine, and a higher amount of endogenous surfactant protein A (SP-A). We conclude that surfactant treatment before reperfusion does improve the immediate lung transplant function in rats in association with an increase in alveolar surfactant components. More particularly, the amount of (endogenous) SP-A is thought to be crucial for the efficacy of surfactant treatment after lung transplantation.</abstract><cop>New York, NY</cop><pub>American Lung Association</pub><pmid>8564115</pmid><doi>10.1164/ajrccm.153.2.8564115</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Animals Biological and medical sciences Blood Proteins - analysis Bronchoalveolar Lavage Fluid - chemistry Glycoproteins - analysis In Vitro Techniques L-Lactate Dehydrogenase - analysis Lipids - pharmacology Lung - physiopathology Lung Transplantation Male Medical sciences Phospholipids - analysis Proteolipids - analysis Pulmonary Circulation Pulmonary Surfactant-Associated Protein A Pulmonary Surfactant-Associated Proteins Pulmonary Surfactants - analysis Pulmonary Surfactants - pharmacology Rats Rats, Inbred Lew Reperfusion Surface Tension Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the respiratory system |
| title | Surfactant treatment before reperfusion improves the immediate function of lung transplants in rats |
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