Development of electrophoretic conditions for the characterization of protein glycoforms by capillary electrophoresis—electrospray mass spectrometry
A capillary electrophoresis (CE) method using acidic buffers and capillaries coated with Polybrene, a cationic polymer has been developed for the separation of glycoproteins and glycopeptides. Electrophoretic conditions have been optimized to provide resolution of individual glycoforms observed for...
Gespeichert in:
Veröffentlicht in: | Journal of Chromatography A 1996-01, Vol.720 (1), p.409-427 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 427 |
---|---|
container_issue | 1 |
container_start_page | 409 |
container_title | Journal of Chromatography A |
container_volume | 720 |
creator | Kelly, J.F Locke, S.J Ramaley, L Thibault, P |
description | A capillary electrophoresis (CE) method using acidic buffers and capillaries coated with Polybrene, a cationic polymer has been developed for the separation of glycoproteins and glycopeptides. Electrophoretic conditions have been optimized to provide resolution of individual glycoforms observed for different glycoprotein preparations. These conditions were found to be entirely compatible with the operation of electrospray mass spectrometry (ESMS), which facilitated the assignments of possible carbohydrate compositions of glycopeptides arising from digests of glycoproteins. By using operating conditions enhancing the formation of oxonium fragment ions prior to mass spectral analysis, selective identification of glycopeptides was achieved for complex samples such as those from proteolytic digests or chemical cleavages. Examples of applications are presented for ribonuclease B, ovalbumin, horseradish peroxidase, and a lectin from
Erithrina corallodendron using both CE-ESMS and CE with ultraviolet detection (CE-UV). |
doi_str_mv | 10.1016/0021-9673(94)01197-4 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_77972389</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0021967394011974</els_id><sourcerecordid>77972389</sourcerecordid><originalsourceid>FETCH-LOGICAL-c423t-ffd6c9a75de68a4b5e8d9ac41ac3d9d6a6966a4d3547aafa778581cbc6609d2f3</originalsourceid><addsrcrecordid>eNp9UU1P3DAU9IEKKPQftJJPqBxS7MRrxxekCuiHhMSlPVve55euqyRObS9SeuqPqPiB_SV12BUSF06WZuaN38wj5C1nHzjj8oKxmldaqua9FueMc60qcUCOn-Aj8jqln4xxxVR9SA5byXjNxDF5uMZ77MM04Jhp6Cj2CDmGaRMiZg8Uwuh89mFMtAuR5g1S2NhoIWP0v-3CLGNTDBn9SH_0M4QiHBJdzxTs5PvexvmZbfLp35-_eyRN0c50sCnRND0iA-Y4n5JXne0Tvtm_J-T7p5tvV1-q27vPX68-3lYg6iZXXeckaKtWDmVrxXqFrdMWBLfQOO2klVpKK1yzEsrazirVrloOa5CSaVd3zQk52_mWAL-2mLIZfAIsS48YtskopVXdtLoIxU4IZecUsTNT9EOJZjgzywnM0rVZujZamMcTGFHG3u39t-sB3dPQvv_CX-54LCHvPUaTwOMI6HwsbRgX_Msf_AfE559c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77972389</pqid></control><display><type>article</type><title>Development of electrophoretic conditions for the characterization of protein glycoforms by capillary electrophoresis—electrospray mass spectrometry</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Kelly, J.F ; Locke, S.J ; Ramaley, L ; Thibault, P</creator><creatorcontrib>Kelly, J.F ; Locke, S.J ; Ramaley, L ; Thibault, P</creatorcontrib><description>A capillary electrophoresis (CE) method using acidic buffers and capillaries coated with Polybrene, a cationic polymer has been developed for the separation of glycoproteins and glycopeptides. Electrophoretic conditions have been optimized to provide resolution of individual glycoforms observed for different glycoprotein preparations. These conditions were found to be entirely compatible with the operation of electrospray mass spectrometry (ESMS), which facilitated the assignments of possible carbohydrate compositions of glycopeptides arising from digests of glycoproteins. By using operating conditions enhancing the formation of oxonium fragment ions prior to mass spectral analysis, selective identification of glycopeptides was achieved for complex samples such as those from proteolytic digests or chemical cleavages. Examples of applications are presented for ribonuclease B, ovalbumin, horseradish peroxidase, and a lectin from
Erithrina corallodendron using both CE-ESMS and CE with ultraviolet detection (CE-UV).</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/0021-9673(94)01197-4</identifier><identifier>PMID: 8601204</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Carbohydrate Conformation ; Carbohydrate Sequence ; Electrophoresis, Capillary - methods ; Glycoproteins - analysis ; Hydrolysis ; Mass Spectrometry - methods ; Molecular Sequence Data ; Ovalbumin - analysis ; Ribonucleases - analysis ; Spectrophotometry, Ultraviolet</subject><ispartof>Journal of Chromatography A, 1996-01, Vol.720 (1), p.409-427</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-ffd6c9a75de68a4b5e8d9ac41ac3d9d6a6966a4d3547aafa778581cbc6609d2f3</citedby><cites>FETCH-LOGICAL-c423t-ffd6c9a75de68a4b5e8d9ac41ac3d9d6a6966a4d3547aafa778581cbc6609d2f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0021-9673(94)01197-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8601204$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kelly, J.F</creatorcontrib><creatorcontrib>Locke, S.J</creatorcontrib><creatorcontrib>Ramaley, L</creatorcontrib><creatorcontrib>Thibault, P</creatorcontrib><title>Development of electrophoretic conditions for the characterization of protein glycoforms by capillary electrophoresis—electrospray mass spectrometry</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>A capillary electrophoresis (CE) method using acidic buffers and capillaries coated with Polybrene, a cationic polymer has been developed for the separation of glycoproteins and glycopeptides. Electrophoretic conditions have been optimized to provide resolution of individual glycoforms observed for different glycoprotein preparations. These conditions were found to be entirely compatible with the operation of electrospray mass spectrometry (ESMS), which facilitated the assignments of possible carbohydrate compositions of glycopeptides arising from digests of glycoproteins. By using operating conditions enhancing the formation of oxonium fragment ions prior to mass spectral analysis, selective identification of glycopeptides was achieved for complex samples such as those from proteolytic digests or chemical cleavages. Examples of applications are presented for ribonuclease B, ovalbumin, horseradish peroxidase, and a lectin from
Erithrina corallodendron using both CE-ESMS and CE with ultraviolet detection (CE-UV).</description><subject>Carbohydrate Conformation</subject><subject>Carbohydrate Sequence</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Glycoproteins - analysis</subject><subject>Hydrolysis</subject><subject>Mass Spectrometry - methods</subject><subject>Molecular Sequence Data</subject><subject>Ovalbumin - analysis</subject><subject>Ribonucleases - analysis</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1P3DAU9IEKKPQftJJPqBxS7MRrxxekCuiHhMSlPVve55euqyRObS9SeuqPqPiB_SV12BUSF06WZuaN38wj5C1nHzjj8oKxmldaqua9FueMc60qcUCOn-Aj8jqln4xxxVR9SA5byXjNxDF5uMZ77MM04Jhp6Cj2CDmGaRMiZg8Uwuh89mFMtAuR5g1S2NhoIWP0v-3CLGNTDBn9SH_0M4QiHBJdzxTs5PvexvmZbfLp35-_eyRN0c50sCnRND0iA-Y4n5JXne0Tvtm_J-T7p5tvV1-q27vPX68-3lYg6iZXXeckaKtWDmVrxXqFrdMWBLfQOO2klVpKK1yzEsrazirVrloOa5CSaVd3zQk52_mWAL-2mLIZfAIsS48YtskopVXdtLoIxU4IZecUsTNT9EOJZjgzywnM0rVZujZamMcTGFHG3u39t-sB3dPQvv_CX-54LCHvPUaTwOMI6HwsbRgX_Msf_AfE559c</recordid><startdate>19960112</startdate><enddate>19960112</enddate><creator>Kelly, J.F</creator><creator>Locke, S.J</creator><creator>Ramaley, L</creator><creator>Thibault, P</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960112</creationdate><title>Development of electrophoretic conditions for the characterization of protein glycoforms by capillary electrophoresis—electrospray mass spectrometry</title><author>Kelly, J.F ; Locke, S.J ; Ramaley, L ; Thibault, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-ffd6c9a75de68a4b5e8d9ac41ac3d9d6a6966a4d3547aafa778581cbc6609d2f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Carbohydrate Conformation</topic><topic>Carbohydrate Sequence</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Glycoproteins - analysis</topic><topic>Hydrolysis</topic><topic>Mass Spectrometry - methods</topic><topic>Molecular Sequence Data</topic><topic>Ovalbumin - analysis</topic><topic>Ribonucleases - analysis</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kelly, J.F</creatorcontrib><creatorcontrib>Locke, S.J</creatorcontrib><creatorcontrib>Ramaley, L</creatorcontrib><creatorcontrib>Thibault, P</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kelly, J.F</au><au>Locke, S.J</au><au>Ramaley, L</au><au>Thibault, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of electrophoretic conditions for the characterization of protein glycoforms by capillary electrophoresis—electrospray mass spectrometry</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>1996-01-12</date><risdate>1996</risdate><volume>720</volume><issue>1</issue><spage>409</spage><epage>427</epage><pages>409-427</pages><issn>0021-9673</issn><abstract>A capillary electrophoresis (CE) method using acidic buffers and capillaries coated with Polybrene, a cationic polymer has been developed for the separation of glycoproteins and glycopeptides. Electrophoretic conditions have been optimized to provide resolution of individual glycoforms observed for different glycoprotein preparations. These conditions were found to be entirely compatible with the operation of electrospray mass spectrometry (ESMS), which facilitated the assignments of possible carbohydrate compositions of glycopeptides arising from digests of glycoproteins. By using operating conditions enhancing the formation of oxonium fragment ions prior to mass spectral analysis, selective identification of glycopeptides was achieved for complex samples such as those from proteolytic digests or chemical cleavages. Examples of applications are presented for ribonuclease B, ovalbumin, horseradish peroxidase, and a lectin from
Erithrina corallodendron using both CE-ESMS and CE with ultraviolet detection (CE-UV).</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8601204</pmid><doi>10.1016/0021-9673(94)01197-4</doi><tpages>19</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0021-9673 |
ispartof | Journal of Chromatography A, 1996-01, Vol.720 (1), p.409-427 |
issn | 0021-9673 |
language | eng |
recordid | cdi_proquest_miscellaneous_77972389 |
source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Carbohydrate Conformation Carbohydrate Sequence Electrophoresis, Capillary - methods Glycoproteins - analysis Hydrolysis Mass Spectrometry - methods Molecular Sequence Data Ovalbumin - analysis Ribonucleases - analysis Spectrophotometry, Ultraviolet |
title | Development of electrophoretic conditions for the characterization of protein glycoforms by capillary electrophoresis—electrospray mass spectrometry |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T01%3A18%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20electrophoretic%20conditions%20for%20the%20characterization%20of%20protein%20glycoforms%20by%20capillary%20electrophoresis%E2%80%94electrospray%20mass%20spectrometry&rft.jtitle=Journal%20of%20Chromatography%20A&rft.au=Kelly,%20J.F&rft.date=1996-01-12&rft.volume=720&rft.issue=1&rft.spage=409&rft.epage=427&rft.pages=409-427&rft.issn=0021-9673&rft_id=info:doi/10.1016/0021-9673(94)01197-4&rft_dat=%3Cproquest_cross%3E77972389%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=77972389&rft_id=info:pmid/8601204&rft_els_id=0021967394011974&rfr_iscdi=true |