Cloning of a pectate lyase gene from Xanthomonas campestris pv. malvacearum and comparison of its sequence relationship with pel genes of soft-rot Erwinia and Pseudomonas

The cotton blight pathogen, Xanthomonas campestris pv. malvacearum strain B414, produces an extracellular pectate lyase (Pel) with an estimated Mr of 41,000 and pI of 9.7. The gene coding for this enzyme initially identified in a 1.8-kb PstI genomic DNA fragment was cloned. The nucleotide sequences...

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Veröffentlicht in:	Molecular plant-microbe interactions 1996-01, Vol.9 (1), p.14-21
Hauptverfasser:	Liao, C.H. (Eastern Regional Research Center, USDA, ARS, Philadelphia, PA.), Gaffney, T.D, Bradley, S.P, Wong, L.J.C
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Amino Acid Sequence Bacterial plant pathogens Base Sequence Biological and medical sciences CLONACION CLONAGE COMPOSICION QUIMICA COMPOSITION CHIMIQUE Erwinia - enzymology Erwinia - genetics ERWINIA CHRYSANTHEMI Escherichia coli - genetics Fundamental and applied biological sciences. Psychology GENE GENES Genes, Bacterial Genomic Library LIASAS LYASE Molecular Sequence Data Phytopathology. Animal pests. Plant and forest protection Plant Diseases - microbiology Polysaccharide-Lyases - genetics Pseudomonas - enzymology Pseudomonas - genetics PSEUDOMONAS FLUORESCENS PSEUDOMONAS VIRIDIFLAVA SECUENCIA NUCLEICA Sequence Analysis, DNA Sequence Homology, Amino Acid SEQUENCE NUCLEIQUE Species Specificity Systematics. Structure, properties and multiplication. Genetics Vegetables - microbiology Virulence - genetics XANTHOMONAS CAMPESTRIS Xanthomonas campestris - enzymology Xanthomonas campestris - genetics Xanthomonas campestris - pathogenicity
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