Determination of lymphocyte immunophenotypic values for normal full-term cord blood
Normal reference values for flow cytometric immunophenotypic lymphocyte markers for cord blood (CB) were determined using sufficient numbers of subjects for clinical laboratory use. Samples from 202 normal gestations were processed by whole blood lysis and analyzed in the following combinations: CD1...
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| Veröffentlicht in: | American journal of clinical pathology 1996, Vol.105 (1), p.38-43 |
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| container_title | American journal of clinical pathology |
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| creator | MOTLEY, D MEYER, M. P KING, R. A NAUS, G. J |
| description | Normal reference values for flow cytometric immunophenotypic lymphocyte markers for cord blood (CB) were determined using sufficient numbers of subjects for clinical laboratory use. Samples from 202 normal gestations were processed by whole blood lysis and analyzed in the following combinations: CD14/45, CD4/3, CD8/3, CD45RA/4, CD29/4, CD56/3, Cd19/3, CD19/10. Thirty-five adult laboratory volunteers were analyzed as controls. When compared to adults, CB showed increased relative percentages of naive T-helper cells, B cells, immature B cells, and CD8+3-cells and decreased T cells, cytotoxic T cells, activated T-helper cells, and large granular lymphocytes (CD56+3+). Significant differences were also found when CB samples were stratified by sex and race. These results provide clinical laboratory normal reference values for lymphocyte markers for CB, demonstrate the need for determining separate standard reference values for significantly different patient populations, provide the basis for future investigation of pathologic gestations and for clinical laboratory applications, and provide insight into early immunologic development. |
| doi_str_mv | 10.1093/ajcp/105.1.38 |
| format | Article |
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P ; KING, R. A ; NAUS, G. J</creator><creatorcontrib>MOTLEY, D ; MEYER, M. P ; KING, R. A ; NAUS, G. J</creatorcontrib><description>Normal reference values for flow cytometric immunophenotypic lymphocyte markers for cord blood (CB) were determined using sufficient numbers of subjects for clinical laboratory use. Samples from 202 normal gestations were processed by whole blood lysis and analyzed in the following combinations: CD14/45, CD4/3, CD8/3, CD45RA/4, CD29/4, CD56/3, Cd19/3, CD19/10. Thirty-five adult laboratory volunteers were analyzed as controls. When compared to adults, CB showed increased relative percentages of naive T-helper cells, B cells, immature B cells, and CD8+3-cells and decreased T cells, cytotoxic T cells, activated T-helper cells, and large granular lymphocytes (CD56+3+). Significant differences were also found when CB samples were stratified by sex and race. These results provide clinical laboratory normal reference values for lymphocyte markers for CB, demonstrate the need for determining separate standard reference values for significantly different patient populations, provide the basis for future investigation of pathologic gestations and for clinical laboratory applications, and provide insight into early immunologic development.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1093/ajcp/105.1.38</identifier><identifier>PMID: 8561086</identifier><identifier>CODEN: AJCPAI</identifier><language>eng</language><publisher>Chicago, IL: American Society of Clinical Pathologists</publisher><subject>Adolescent ; Adult ; Antigens, CD - analysis ; Biological and medical sciences ; Blood Cell Count ; Female ; Fetal Blood - cytology ; Flow Cytometry ; Gynecology. Andrology. Obstetrics ; Humans ; Immunophenotyping ; Infant, Newborn ; Lymphocyte Subsets ; Lymphocytes - classification ; Lymphocytes - immunology ; Male ; Management. Prenatal diagnosis ; Medical sciences ; Middle Aged ; Pregnancy. Fetus. Placenta ; Reference Values ; Sex Characteristics</subject><ispartof>American journal of clinical pathology, 1996, Vol.105 (1), p.38-43</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-9ace1b643b68f83e7ea3fa479e32a2d8b5fc8b4c6e15370e14e65275f6c76b8a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2976358$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8561086$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MOTLEY, D</creatorcontrib><creatorcontrib>MEYER, M. P</creatorcontrib><creatorcontrib>KING, R. A</creatorcontrib><creatorcontrib>NAUS, G. J</creatorcontrib><title>Determination of lymphocyte immunophenotypic values for normal full-term cord blood</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>Normal reference values for flow cytometric immunophenotypic lymphocyte markers for cord blood (CB) were determined using sufficient numbers of subjects for clinical laboratory use. Samples from 202 normal gestations were processed by whole blood lysis and analyzed in the following combinations: CD14/45, CD4/3, CD8/3, CD45RA/4, CD29/4, CD56/3, Cd19/3, CD19/10. Thirty-five adult laboratory volunteers were analyzed as controls. When compared to adults, CB showed increased relative percentages of naive T-helper cells, B cells, immature B cells, and CD8+3-cells and decreased T cells, cytotoxic T cells, activated T-helper cells, and large granular lymphocytes (CD56+3+). Significant differences were also found when CB samples were stratified by sex and race. These results provide clinical laboratory normal reference values for lymphocyte markers for CB, demonstrate the need for determining separate standard reference values for significantly different patient populations, provide the basis for future investigation of pathologic gestations and for clinical laboratory applications, and provide insight into early immunologic development.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Antigens, CD - analysis</subject><subject>Biological and medical sciences</subject><subject>Blood Cell Count</subject><subject>Female</subject><subject>Fetal Blood - cytology</subject><subject>Flow Cytometry</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunophenotyping</subject><subject>Infant, Newborn</subject><subject>Lymphocyte Subsets</subject><subject>Lymphocytes - classification</subject><subject>Lymphocytes - immunology</subject><subject>Male</subject><subject>Management. Prenatal diagnosis</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Reference Values</subject><subject>Sex Characteristics</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLxDAUhYMo4zi6dClkIe46kzTNo0vxDQMu1HVJ0xumQ9rUpBX67-0wZVb3wPn4uByEbilZU5Kzjd6bbkMJX9M1U2doSfOMJVKm6TlaEkLSJKeSXaKrGPeE0FSRbIEWigtKlFiir2foITR1q_vat9hb7Mam23kz9oDrphla3-2g9f3Y1Qb_aTdAxNYH3PrQaIft4FxyMGDjQ4VL5311jS6sdhFu5rtCP68v30_vyfbz7ePpcZsYxkWf5NoALUXGSqGsYiBBM6szmQNLdVqpklujyswIoJxJAjQDwVPJrTBSlEqzFXo4ervgf6e_-qKpowHndAt-iIWUuaSSywlMjqAJPsYAtuhC3egwFpQUhxGLw4hT4gUtmJr4u1k8lA1UJ3peberv515Ho50NujV1PGFpLgXjiv0Dp6B8HA</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>MOTLEY, D</creator><creator>MEYER, M. P</creator><creator>KING, R. A</creator><creator>NAUS, G. J</creator><general>American Society of Clinical Pathologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>Determination of lymphocyte immunophenotypic values for normal full-term cord blood</title><author>MOTLEY, D ; MEYER, M. P ; KING, R. A ; NAUS, G. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-9ace1b643b68f83e7ea3fa479e32a2d8b5fc8b4c6e15370e14e65275f6c76b8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Antigens, CD - analysis</topic><topic>Biological and medical sciences</topic><topic>Blood Cell Count</topic><topic>Female</topic><topic>Fetal Blood - cytology</topic><topic>Flow Cytometry</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunophenotyping</topic><topic>Infant, Newborn</topic><topic>Lymphocyte Subsets</topic><topic>Lymphocytes - classification</topic><topic>Lymphocytes - immunology</topic><topic>Male</topic><topic>Management. Prenatal diagnosis</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Reference Values</topic><topic>Sex Characteristics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MOTLEY, D</creatorcontrib><creatorcontrib>MEYER, M. P</creatorcontrib><creatorcontrib>KING, R. A</creatorcontrib><creatorcontrib>NAUS, G. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MOTLEY, D</au><au>MEYER, M. P</au><au>KING, R. A</au><au>NAUS, G. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of lymphocyte immunophenotypic values for normal full-term cord blood</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>1996</date><risdate>1996</risdate><volume>105</volume><issue>1</issue><spage>38</spage><epage>43</epage><pages>38-43</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><coden>AJCPAI</coden><abstract>Normal reference values for flow cytometric immunophenotypic lymphocyte markers for cord blood (CB) were determined using sufficient numbers of subjects for clinical laboratory use. Samples from 202 normal gestations were processed by whole blood lysis and analyzed in the following combinations: CD14/45, CD4/3, CD8/3, CD45RA/4, CD29/4, CD56/3, Cd19/3, CD19/10. Thirty-five adult laboratory volunteers were analyzed as controls. When compared to adults, CB showed increased relative percentages of naive T-helper cells, B cells, immature B cells, and CD8+3-cells and decreased T cells, cytotoxic T cells, activated T-helper cells, and large granular lymphocytes (CD56+3+). Significant differences were also found when CB samples were stratified by sex and race. These results provide clinical laboratory normal reference values for lymphocyte markers for CB, demonstrate the need for determining separate standard reference values for significantly different patient populations, provide the basis for future investigation of pathologic gestations and for clinical laboratory applications, and provide insight into early immunologic development.</abstract><cop>Chicago, IL</cop><pub>American Society of Clinical Pathologists</pub><pmid>8561086</pmid><doi>10.1093/ajcp/105.1.38</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | American journal of clinical pathology, 1996, Vol.105 (1), p.38-43 |
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| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Adolescent Adult Antigens, CD - analysis Biological and medical sciences Blood Cell Count Female Fetal Blood - cytology Flow Cytometry Gynecology. Andrology. Obstetrics Humans Immunophenotyping Infant, Newborn Lymphocyte Subsets Lymphocytes - classification Lymphocytes - immunology Male Management. Prenatal diagnosis Medical sciences Middle Aged Pregnancy. Fetus. Placenta Reference Values Sex Characteristics |
| title | Determination of lymphocyte immunophenotypic values for normal full-term cord blood |
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