Double long-chain amidine liposome-mediated self replicating RNA transfection
We present experimental evidence that a complex made of a double long chain cationic amphiphile and recombinant mRNA facilitates the entry and expression of genetic material into cells. Combining the properties of the self replicating recombinant mRNA driven by the Semliki Forest Virus (SFV) replico...
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| Veröffentlicht in: | FEBS letters 1996-02, Vol.380 (1-2), p.108-112 |
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| container_issue | 1-2 |
| container_start_page | 108 |
| container_title | FEBS letters |
| container_volume | 380 |
| creator | El Ouahabi, A Pector, V Fuks, R Vandenbranden, M Ruysschaert, J M |
| description | We present experimental evidence that a complex made of a double long chain cationic amphiphile and recombinant mRNA facilitates the entry and expression of genetic material into cells. Combining the properties of the self replicating recombinant mRNA driven by the Semliki Forest Virus (SFV) replicon and the transfection potentialities of a new cationic amphiphile (N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine) yields a highly efficient mRNA transfection system conferring up to 100% infectivity. The preparation and characterization of the long chain amidine cationic amphiphile-mRNA complex as well as the influence of the diC14-amidine/RNA ratio on the infective activity are described. |
| doi_str_mv | 10.1016/0014-5793(96)00038-5 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1996-02, Vol.380 (1-2), p.108-112 |
| issn | 0014-5793 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_77971577 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amidines - chemistry Animals beta-Galactosidase - genetics beta-Galactosidase - metabolism Bromovirus - genetics Cell Line Cricetinae Liposomes Protein Biosynthesis Ribonuclease, Pancreatic - metabolism RNA - chemistry RNA - metabolism RNA, Messenger - chemistry RNA, Messenger - genetics RNA, Messenger - metabolism Semliki Forest virus Transfection - methods |
| title | Double long-chain amidine liposome-mediated self replicating RNA transfection |
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